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Product Info Summary
| SKU: | PB9500 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PLK1 Antibody Picoband®
SKU/Catalog Number
PB9500
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PLK1 Antibody Picoband® catalog # PB9500. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9500)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PLK1 recombinant protein (Position: K86-N430). Human PLK1 shares 95.4% and 96.2% amino acid (aa) sequence identity with mouse and rat PLK1, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9500 is reactive to PLK1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
68.3 kDa
Background of PLK1
Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9500 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, PANC Whole Cell
Validation Images & Assay Conditions
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Western blot analysis of PLK1 using anti-PLK1 antibody (PB9500).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug,
Lane 2: PANC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLK1 antigen affinity purified polyclonal antibody (Catalog # PB9500) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLK1 at approximately 68 kDa. The expected band size for PLK1 is at 68 kDa.
Specific Publications For Anti-PLK1 Antibody Picoband® (PB9500)
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5 Customer Q&As for Anti-PLK1 Antibody Picoband®
Question
I was wanting to use using your anti-PLK1 antibody for regulation of cell cycle g2/m phase transition studies. Has this antibody been tested with western blotting on panc whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Thanks for your inquiry. This PB9500 anti-PLK1 antibody is tested on hela whole cell lysate, panc whole cell lysate, mouse lymphaden tissue, rat lymphaden tissue, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-21
Question
We were satisfied with the WB result of your anti-PLK1 antibody. However we have been able to see positive staining in cervix carcinoma centromere, using this antibody. Is that expected? Could you tell me where is PLK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
From literature, cervix carcinoma does express PLK1. Generally PLK1 expresses in nucleus. chromosome, centromere,. Regarding which tissues have PLK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, and Lung, Pubmed ID: 15489334
Lung, Pubmed ID: 8127874
Lymphoblast, Pubmed ID: 14654843
Placenta, Pubmed ID: 8018557, 7902533
Boster Scientific Support
Answered: 2018-09-03
Question
We are currently using anti-PLK1 antibody PB9500 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
The anti-PLK1 antibody (PB9500) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-13
Question
We have tried in the past anti-PLK1 antibody for IHC on lymphoblast in a previous project. I am using rat, and We are going to use the antibody for WB next. We want examining lymphoblast as well as colon lung in our next experiment. Could give a recommendation on which antibody would work the best for WB?
G. Jones
Verified customer
Asked: 2015-01-12
Answer
I viewed the website and datasheets of our anti-PLK1 antibody and it seems that PB9500 has been tested on rat in both IHC and WB. Thus PB9500 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-12
Question
We have seen staining in mouse cervix carcinoma. Are there any suggestions? Is anti-PLK1 antibody supposed to stain cervix carcinoma positively?
B. Li
Verified customer
Asked: 2013-09-17
Answer
Based on literature cervix carcinoma does express PLK1. Based on Uniprot.org, PLK1 is expressed in testis, placenta, lung, colon lung, lymphoblast, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PLK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, and Lung, Pubmed ID: 15489334
Lung, Pubmed ID: 8127874
Lymphoblast, Pubmed ID: 14654843
Placenta, Pubmed ID: 8018557, 7902533
Boster Scientific Support
Answered: 2013-09-17
