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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human PLA2G2A ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human PLA2G2A ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLA2G2A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human PLA2G2A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1944)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: sf21; Immunogen sequence: N21-C144
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1944 is reactive to PLA2G2A in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1944 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1944-CAP||Anti-Human PLA2G2A Pre-coated 96-well strip microplate||1|
|EK1944-ST||Human PLA2G2A Standard||2 vials, 10 ng/tube|
|EK1944-DA||Human PLA2G2A Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human PLA2G2A PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PLA2G2A, Dilution ratio of 1:1, concentration in serum is around 6ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PLA2G2A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of PLA2G2A in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PLA2G2A (Source: Uniprot.Org, NCBI)
Phospholipase A2, membrane associated
phospholipase A2 family
EC 184.108.40.206; GIIC sPLA2; Group IIA phospholipase A2; Mom1; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLAS1; RASF-A; sPLA2 PLA2G2A MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2 phospholipase A2 group IIA phospholipase A2, membrane associated|GIIC sPLA2|NPS-PLA2|group IIA phospholipase A2|non-pancreatic secretory phospholipase A2|phosphatidylcholine 2-acylhydrolase 2A|phospholipase A2, group IIA (platelets, synovial fluid)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PLA2G2A, check out the PLA2G2A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLA2G2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1944
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