Click image to see more details
Product Info Summary
| SKU: | A05768 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Rab9/RAB9A Antibody Picoband®
SKU/Catalog Number
A05768
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rab9/RAB9A Antibody Picoband® catalog # A05768. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab9/RAB9A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05768)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Rab9/RAB9A recombinant protein (Position: K4-D171).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A05768 is reactive to RAB9A in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
22.8 kDa
Background of RAB9A
Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What’s more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05768 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human K562 whole cell, human HepG2 whole cell, human HEK293 whole cell, human Hela whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
ICC/IF: A431 cell
FCM: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (A05768).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab9/RAB9A antigen affinity purified polyclonal antibody (Catalog # A05768) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab9/RAB9A at approximately 23KD. The expected band size for Rab9/RAB9A is at 23KD.
Click image to see more details
IF analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (A05768).
Rab9/RAB9A was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Rab9/RAB9A Antibody (A05768) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Flow Cytometry analysis of Hela cells using anti-Rab9/RAB9A antibody (A05768).
Overlay histogram showing Hela cells stained with A05768 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rab9/RAB9A Antibody (A05768,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Rab9/RAB9A Antibody Picoband® (A05768)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Rab9/RAB9A Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Rab9/RAB9A Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
