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Product Info Summary
| SKU: | M00132 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CTCF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00132
BM4992 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CTCF Rabbit Monoclonal Antibody catalog # M00132. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTCF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00132)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAAE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CTCF
Reactive Species
M00132 is reactive to CTCF in Human, Mouse, Rat
Observed Molecular Weight
150 kDa
Calculated molecular weight
82.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00132 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human MCF-7 whole cell, human SIHA whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of CTCF using anti-CTCF antibody (M00132).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTCF antigen affinity purified monoclonal antibody (M00132) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTCF at approximately 150 kDa. The expected band size for CTCF is at 83 kDa.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Specific Publications For Anti-CTCF Rabbit Monoclonal Antibody (M00132)
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5 Customer Q&As for Anti-CTCF Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-CTCF Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell nucleus using this antibody. Is that expected? Could you tell me where is CTCF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
From literature, leukemic t-cell does express CTCF. Generally CTCF expresses in nucleus, nucleoplasm. Regarding which tissues have CTCF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15616553
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-21
Question
We have been able to see staining in rat placenta. Any tips? Is anti-CTCF Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
From what I have seen in literature placenta does express CTCF. From what I have seen in Uniprot.org, CTCF is expressed in amniotic fluid, brain, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CTCF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15616553
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-20
Question
My question regards using your anti-CTCF Rabbit Monoclonal antibody for centromeric region studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
Thanks for your inquiry. This M00132 anti-CTCF Rabbit Monoclonal antibody is tested on hela cell lysate, mouse brain. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-01
Question
We bought anti-CTCF Rabbit Monoclonal antibody for WB on leukemic t-cell in a previous project. I am using human, and I plan to use the antibody for IF next. My lab would like examining leukemic t-cell as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
M. Krishna
Verified customer
Asked: 2017-04-17
Answer
I looked at the website and datasheets of our anti-CTCF Rabbit Monoclonal antibody and it seems that M00132 has been tested on human in both WB and IF. Thus M00132 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-04-17
Question
We are currently using anti-CTCF Rabbit Monoclonal antibody M00132 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
M. Baker
Verified customer
Asked: 2016-09-05
Answer
The anti-CTCF Rabbit Monoclonal antibody (M00132) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-05
