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Product Info Summary
| SKU: | M00132-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-CTCF Antibody
SKU/Catalog Number
M00132-2
Size
50 µl
Description
Boster Bio Anti-CTCF Antibody (Catalog # M00132-2). Tested in WB application(s). This antibody reacts with Human, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CTCF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00132-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1723CT382.14.9
Isotype
IgG1,k
Immunogen
This CTCF antibody is generated from a mouse immunized with a recombinant protein between 445-727 amino acids from human CTCF.
Reactive Species
M00132-2 is reactive to CTCF in Human, Rat
Calculated molecular weight
82.8 kDa
Background of CTCF
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00132-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-CTCF Antibody at 1:4000 dilution
Lane 1: K562 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: C6 whole cell lysate
Lane 5: MOLT-4 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 83 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-CTCF Antibody (M00132-2)
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