Anti-CTCF Antibody

CTCF antibody

Boster Bio Anti-CTCF Antibody (Catalog # M00132-2). Tested in WB application(s). This antibody reacts with Human, Rat.

Product Info Summary

SKU: M00132-2
Size: 50 µl
Reactive Species: Human, Rat
Host: Mouse
Application: WB

Product Name

Anti-CTCF Antibody

View all CTCF Antibodies

SKU/Catalog Number

M00132-2

Size

50 µl

Description

Boster Bio Anti-CTCF Antibody (Catalog # M00132-2). Tested in WB application(s). This antibody reacts with Human, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-CTCF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00132-2)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1723CT382.14.9

Isotype

IgG1,k

Immunogen

This CTCF antibody is generated from a mouse immunized with a recombinant protein between 445-727 amino acids from human CTCF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00132-2 is reactive to CTCF in Human, Rat

Applications

M00132-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

82.785kDa

Background of CTCF

Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:4000

Validation Images & Assay Conditions

Gene/Protein Information For CTCF (Source: Uniprot.org, NCBI)

Gene Name

CTCF

Full Name

Transcriptional repressor CTCF

Weight

82.785kDa

Superfamily

CTCF zinc-finger protein family

Alternative Names

11 zinc finger transcriptional repressor; 11-zinc finger protein; 11-Zinc Finger Protein2; CCCTC-binding factor (zinc finger protein); CCCTC-binding Factor; CTCF; CTCFL paralog; transcriptional repressor CTCF CTCF CFAP108, FAP108, MRD21 CCCTC-binding factor transcriptional repressor CTCF|11 zinc finger transcriptional repressor|11-zinc finger protein|CCCTC-binding factor (zinc finger protein)|CTCFL paralog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CTCF, check out the CTCF Infographic

CTCF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CTCF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00132-2-50ul

50 µl

$298
M00132-2-200ul

200 µl

$460

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M00132-2
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