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Product Info Summary
| SKU: | M02571-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-MBD3 Monoclonal Antibody
SKU/Catalog Number
M02571-1
BM5735 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MBD3 Monoclonal Antibody catalog # M02571-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02571-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFEE-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MBD3 Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
Reactive Species
M02571-1 is reactive to MBD3 in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
32.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02571-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of MBD3 expression in 293T cell lysate.
Specific Publications For Anti-MBD3 Monoclonal Antibody (M02571-1)
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1 Customer Q&As for Anti-MBD3 Monoclonal Antibody
Question
We are currently using anti-MBD3 Monoclonal antibody M02571-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-MBD3 Monoclonal antibody (M02571-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
