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Product Info Summary
| SKU: | M00745-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IP, IF, WB |
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Product info
Product Name
Anti-Cyclin B1 CCNB1 Monoclonal Antibody
SKU/Catalog Number
M00745-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cyclin B1 CCNB1 Monoclonal Antibody catalog # M00745-1. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin B1 CCNB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5G6
Isotype
IgG
Immunogen
Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00745-1 is reactive to CCNB1 in Human, Rat
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00745-1 is guaranteed for ELISA, Flow Cytometry, IP, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis using Cyclin B1 Monoclonal Antibody against HeLa (1) and PC-12 (2) cell lysate.
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Immunofluorescence analysis of Hela cells using Cyclin B1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of Hela cells using Cyclin B1 Monoclonal Antibody (green) and negative control (purple).
Specific Publications For Anti-Cyclin B1 CCNB1 Monoclonal Antibody (M00745-1)
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