Anti-Cyclin B1 CCNB1 Monoclonal Antibody

Cyclin B1 antibody

Boster Bio Anti-Cyclin B1 CCNB1 Monoclonal Antibody catalog # M00745-1. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: M00745-1
Size: 100ul
Reactive Species: Human, Rat
Host: Mouse
Application: ELISA, Flow Cytometry, IF, WB

Product Name

Anti-Cyclin B1 CCNB1 Monoclonal Antibody

View all Cyclin B1 Antibodies

SKU/Catalog Number

M00745-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Cyclin B1 CCNB1 Monoclonal Antibody catalog # M00745-1. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin B1 CCNB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-1)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

5G6

Isotype

IgG

Immunogen

Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00745-1 is reactive to CCNB1 in Human, Rat

Applications

M00745-1 is guaranteed for ELISA, Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

48.337kDa

Background of Cyclin B1

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000

Validation Images & Assay Conditions

Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)

Gene Name

CCNB1

Full Name

G2/mitotic-specific cyclin-B1

Weight

48.337kDa

Superfamily

cyclin family

Alternative Names

CCNB; CCNB1; Cyclin B1; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1 CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCNB1, check out the CCNB1 Infographic

CCNB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00745-1 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang J,Wang L,Chen S,Peng H,Xiao L,E Du,Liu Y,Lin D,Wang Y,Xu Y,Yang K.PKMYT1 is associated with prostate cancer malignancy and may serve as a therapeutic target.Gene.2020 Jun 20;744:144608.doi:10.1016/j. gene.2020.144608.Epub 2020 Mar 29.PMID:32234541.
Species: Human
M00745-1 usage in article: APP:WB, SAMPLE:LNCAP CELL,C4-2 CELL,PC-3 CELL AND DU 145 CELL, DILUTION:1:200

mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation

Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.

Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

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Order DetailsPrice
M00745-1-100ul

100ul

$380

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$380.00

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