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- Table of Contents
MKI67 antibody (M00254) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
Filter:
Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Anti-Ki67 MKI67 Rabbit Monoclonal Antibody
MKI67 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
MKI67 antibody was positively detected by WB in:
Ramos cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MKI67 antibody was positively detected by IHC in:
Human pituitary tumor tissue, Human liver cancer tissue, human tonsil tissue, Human esophageal carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Ki67 MKI67 Rabbit Monoclonal Antibody
View all MKI67 Antibodies
View all MKI67 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EAH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ki67
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
359 kDa
Calculated Molecular Weight
358.7 kDa
Cite This Product
Anti-Ki67 MKI67 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254)
Protocols
Western Blot protocol for MKI67 antibody
Immunohistochemistry (IHC) protocol for MKI67 antibody
Publications
Application:
Species:
Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer
Published: 2022-07-11
Journal: Cell Death Discovery
Application: IHC
Impact Factor: 7.11
Species: Mouse
PMID: 35821230
TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer
Published: 2020-10-22
Journal: Autophagy
Application: WB
Impact Factor: 9.77
Species: Human
PMID: 32972302
LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes
Published: 2020-07-29
Journal: Molecular Cancer
Application: IHC
Impact Factor: 15.30
Species: Human
PMID: 32727463
Review
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Product Promise
We promise Anti-Ki67 MKI67 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00254
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026