Product Info Summary
SKU: | A01357S33 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 18 (phospho-S33) KRT18 Antibody
SKU/Catalog Number
A01357S33
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 18 (phospho-S33) KRT18 Antibody catalog # A01357S33. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 18 (phospho-S33) KRT18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01357S33)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Kpm
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01357S33 is reactive to KRT18 in Human, Mouse, Rat
Applications
A01357S33 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
48.058kDa
Background of KRT18
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Kumagai H., Mol. Cell. Biol. 19:5083-5095(1999).
Jiang W., EMBO J. 18:5703-5713(1999).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of KRT18 using Anti-Cytokeratin 18 (phospho-S33) KRT18 Antibody (A01357S33).
Immunohistochemistry (IHC) analyzes of p-Cytokeratin 18 (S33) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Cytokeratin 18 (phospho-S33) KRT18 Antibody A01357S33
Western blot (WB) analysis of p-Cytokeratin 18 (S33) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate(40ug)
Lane2:L02 whole cell lysate(40ug)
Lane3:MCF-7 whole cell lysate(40ug)
Lane4:CT26 whole cell lysate(40ug)
Lane5:PMVEC whole cell lysate(40ug)
Lane6:A549 whole cell lysate(40ug)
Lane7:SGC7901 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For KRT18 (Source: Uniprot.org, NCBI)
Gene Name
KRT18
Full Name
Keratin, type I cytoskeletal 18
Weight
48.058kDa
Superfamily
intermediate filament family
Alternative Names
Cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; CK-18; CYK18; Cytokeratin 18; cytokeratin-18; K18; Keratin 18; keratin, type I cytoskeletal 18; keratin-18; KRT18 KRT18 CK-18, CYK18, K18 keratin 18 keratin, type I cytoskeletal 18|cell proliferation-inducing gene 46 protein|cytokeratin 18|keratin 18, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT18, check out the KRT18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 18 (phospho-S33) KRT18 Antibody (A01357S33)
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No publications found for A01357S33
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