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Product Info Summary
| SKU: | A00645S2056 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-DNA-PK (phospho-S2056) PRKDC Antibody
SKU/Catalog Number
A00645S2056
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNA-PK (phospho-S2056) PRKDC Antibody catalog # A00645S2056. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA-PK (phospho-S2056) PRKDC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00645S2056)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S2056 of human DNA-PKcs(NP_008835.5).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00645S2056 is reactive to PRKDC in Human, Mouse, Rat
Calculated molecular weight
469.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00645S2056 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of HeLa cells, using Phospho-DNA-PKcs-S2056 antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
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Immunofluorescence analysis of U2OS cells using Phospho-DNA-PKcs-S2056 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Specific Publications For Anti-DNA-PK (phospho-S2056) PRKDC Antibody (A00645S2056)
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