Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody

Boster Bio Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody catalog # P00645. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: P00645
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody

See all DNA-PKcs products

SKU/Catalog Number

P00645

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody catalog # P00645. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00645)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BOD-16

Immunogen

A synthesized peptide derived from human Phospho-DNA PKcs (S2056)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00645 is reactive to PRKDC in Human

Applications

P00645 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For PRKDC (Source: Uniprot.Org, NCBI)

Uniprot ID

P78527

Gene ID

5591

Gene Name

PRKDC

Full Name

DNA-dependent protein kinase catalytic subunit

Weight

469.089kDa

Superfamily

PI3/PI4-kinase family

Alternative Names

DNA-dependent protein kinase catalytic subunit-like; DNA-dependent protein kinase; DNA-PK catalytic subunit; DNAPK; DNAPKcs; DNA-PKcs; DNPK1; EC 2.7.11.1; HYRC; HYRC1; p350; p460; PRKDC; XRCC7 PRKDC DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350 protein kinase, DNA-activated, catalytic subunit DNA-dependent protein kinase catalytic subunit|DNA-PK catalytic subunit|hyper-radiosensitivity of murine scid mutation, complementing 1|p460|protein kinase, DNA-activated, catalytic polypeptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRKDC, check out the PRKDC Infographic

PRKDC infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRKDC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

P00645 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sun JS, Yang XH. Exp Ther Med. 2018 Apr;15(4):3295-3301. doi: 10.3892/etm.2018.5826. Epub 2018 Feb 1. Expression of DNA-dependent protein kinase catalytic subunit in laryngeal squamous cell carcinoma and its importance

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6 Customer Q&As for Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody

Question

What is the antigen retrieval method for P00645?

Verified customer

Asked: 2021-02-19

Answer

Please see the following antigen retrieval method for the Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody (P00645): Heat-induced epitope retrieval: Immerse the slides in antigen retrieval buffer (EDTA buffer, pH 8.0). Microwave the solution at Medium-high power for 8 minutes. Cool the slides for 5 minutes. Microwave the solution at High power for 4 minutes. Cool the slides to room temperature.

Boster Scientific Support

Answered: 2021-02-20

Question

We are currently using anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody P00645 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

The anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody (P00645) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-14

Question

Our lab used your anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody for IHC on cervix carcinoma a few years ago. I am using human, and We want to use the antibody for IF next. We are interested in examining cervix carcinoma as well as leukemic t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2018-10-30

Answer

I looked at the website and datasheets of our anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody and I see that P00645 has been validated on human in both IHC and IF. Thus P00645 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-30

Question

My boss were content with the WB result of your anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody. However we have observed positive staining in brain nucleus using this antibody. Is that expected? Could you tell me where is PRKDC supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-02-02

Answer

Based on literature, brain does express PRKDC. Generally PRKDC expresses in nucleus. Regarding which tissues have PRKDC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2507541, 2247066
Cervix carcinoma, Pubmed ID: 7671312, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal lung, Pubmed ID: 7594449
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7638222

Boster Scientific Support

Answered: 2018-02-02

Question

Our lab want to know about using your anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody for negative regulation of protein phosphorylation studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-01-17

Answer

We appreciate your inquiry. This P00645 anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-01-17

Question

We have seen staining in human brain. Do you have any suggestions? Is anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody supposed to stain brain positively?

D. Huang

Verified customer

Asked: 2013-06-17

Answer

From what I have seen in literature brain does express PRKDC. From what I have seen in Uniprot.org, PRKDC is expressed in kidney, cervix carcinoma, placenta, fetal lung, brain, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PRKDC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2507541, 2247066
Cervix carcinoma, Pubmed ID: 7671312, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal lung, Pubmed ID: 7594449
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7638222

Boster Scientific Support

Answered: 2013-06-17

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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P00645
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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