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Product Info Summary
| SKU: | P00645 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody
SKU/Catalog Number
P00645
BM4058 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody catalog # P00645. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00645)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BOD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-DNA PKcs (S2056)
Reactive Species
P00645 is reactive to PRKDC in Human
Observed Molecular Weight
469 kDa
Calculated molecular weight
469.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00645 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: alkaline treated Jurkat cell
IHC: human colon tissue, human colon carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-DNA PKcs (Ser2056) expression in alkaline treated Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Phospho-DNA PKcs (S2056) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Phospho-DNA PKcs (S2056) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Specific Publications For Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody (P00645)
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody
Question
What is the antigen retrieval method for P00645?
Verified customer
Asked: 2021-02-19
Answer
Please see the following antigen retrieval method for the Anti-Phospho-DNA PKcs (S2056) PRKDC Rabbit Monoclonal Antibody (P00645): Heat-induced epitope retrieval: Immerse the slides in antigen retrieval buffer (EDTA buffer, pH 8.0). Microwave the solution at Medium-high power for 8 minutes. Cool the slides for 5 minutes. Microwave the solution at High power for 4 minutes. Cool the slides to room temperature.
Boster Scientific Support
Answered: 2021-02-20
Question
We are currently using anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody P00645 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
The anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody (P00645) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-14
Question
Our lab used your anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody for IHC on cervix carcinoma a few years ago. I am using human, and We want to use the antibody for IF next. We are interested in examining cervix carcinoma as well as leukemic t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
I looked at the website and datasheets of our anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody and I see that P00645 has been validated on human in both IHC and IF. Thus P00645 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-10-30
Question
My boss were content with the WB result of your anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody. However we have observed positive staining in brain nucleus using this antibody. Is that expected? Could you tell me where is PRKDC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
Based on literature, brain does express PRKDC. Generally PRKDC expresses in nucleus. Regarding which tissues have PRKDC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2507541, 2247066
Cervix carcinoma, Pubmed ID: 7671312, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal lung, Pubmed ID: 7594449
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7638222
Boster Scientific Support
Answered: 2018-02-02
Question
Our lab want to know about using your anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody for negative regulation of protein phosphorylation studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
We appreciate your inquiry. This P00645 anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-17
Question
We have seen staining in human brain. Do you have any suggestions? Is anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal antibody supposed to stain brain positively?
D. Huang
Verified customer
Asked: 2013-06-17
Answer
From what I have seen in literature brain does express PRKDC. From what I have seen in Uniprot.org, PRKDC is expressed in kidney, cervix carcinoma, placenta, fetal lung, brain, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PRKDC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2507541, 2247066
Cervix carcinoma, Pubmed ID: 7671312, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal lung, Pubmed ID: 7594449
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7638222
Boster Scientific Support
Answered: 2013-06-17
