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Product Info Summary
| SKU: | A04220 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Bovine, Mouse, Rat, Xenopus |
| Host: | Goat |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Dnajc5/Csp Antibody
SKU/Catalog Number
A04220
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-Dnajc5/Csp Antibody catalog # A04220. Tested in IP, IHC, WB applications. This antibody reacts with Mouse, Rat, Bovine, Xenopus.
Storage & Handling
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Cite This Product
Anti-Dnajc5/Csp Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04220)
Host
Goat
Contents
Each vial contains containing 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Clone Number
5G7
Isotype
IgG
Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of rat CSP. The sequence is completely conserved in bovine.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04220 is reactive to Dnajc5 in Bovine, Mouse, Rat, Xenopus
Applications
A04220 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.101kDa
Background of CSP
Cysteine string protein (CSP) is a 34 kDa rat brain protein localized to the cytoplasmic surface of the synaptic vesicle. CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, Immunoprecipitation (0.5µg/ml)
Western Blot (1:2,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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CSP was detected in paraffin-embedded sections of xenopus embryonic retina tissues using rabbit anti-CSP Antigen Affinity purified polyclonal antibody (Catalog # A04220) at 0.5 μg/mL.
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Western blot analysis of CSP expression in mouse brain extract (lane 1). CSP at 34KD was detected using rabbit anti-CSP Antigen Affinity purified polyclonal antibody (Catalog # A04220) at 1:2000. The blot was developed using colorimetric method.
Protein Target Info & Infographic
Gene/Protein Information For Dnajc5 (Source: Uniprot.org, NCBI)
Gene Name
Dnajc5
Full Name
DnaJ homolog subfamily C member 5
Weight
22.101kDa
Alternative Names
CSP; cysteine string protein alpha; Cysteine string protein; DKFZp434N1429; DKFZp761N1221; DnaJ (Hsp40) homolog, subfamily C, member 5; dnaJ homolog subfamily C member 5; DNAJC5A; FLJ00118; FLJ13070 Dnajc5|2610314I24Rik, AU018536, C, Csp|DnaJ heat shock protein family (Hsp40) member C5|dnaJ homolog subfamily C member 5|DnaJ (Hsp40) homolog, subfamily C, member 5|cysteine string protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Dnajc5, check out the Dnajc5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Dnajc5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dnajc5/Csp Antibody (A04220)
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No publications found for A04220
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Dnajc5/Csp Antibody
Question
We are currently using anti-Dnajc5/Csp antibody A04220 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The anti-Dnajc5/Csp antibody (A04220) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
I see that the anti-Dnajc5/Csp antibody A04220 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Dnajc5/Csp antibody A04220. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Is this A04220 anti-Dnajc5/Csp antibody reactive to the isotypes of DNAJC5?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The immunogen of A04220 anti-Dnajc5/Csp antibody is Synthetic peptide with sequence C-SATETTQLTADSHPS, corresponding to the C Terminus of the protein sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-27
Question
Is a blocking peptide available for product anti-Dnajc5/Csp antibody (A04220)?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
We do provide the blocking peptide for product anti-Dnajc5/Csp antibody (A04220). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-21
Question
I was wanting to use your anti-Dnajc5/Csp antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can see on the product datasheet, A04220 anti-Dnajc5/Csp antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Can you help my question with product A04220, anti-Dnajc5/Csp antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04220 anti-Dnajc5/Csp antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-24
Question
Does anti-Dnajc5/Csp antibody A04220 work for WB with leukemic t-cell?
K. Li
Verified customer
Asked: 2018-02-23
Answer
According to the expression profile of leukemic t-cell, DNAJC5 is highly expressed in leukemic t-cell. So, it is likely that anti-Dnajc5/Csp antibody A04220 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-02-23
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-Dnajc5/Csp antibody A04220. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-29
Question
Would A04220 anti-Dnajc5/Csp antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
As indicated on the product datasheet, A04220 anti-Dnajc5/Csp antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-15
Question
Do you have a BSA free version of anti-Dnajc5/Csp antibody A04220 available?
V. Kulkarni
Verified customer
Asked: 2017-01-27
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Dnajc5/Csp antibody A04220 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-01-27
Question
Does anti-Dnajc5/Csp antibody A04220 work on zebrafish IHC with spleen?
B. Gonzalez
Verified customer
Asked: 2013-12-19
Answer
Our lab technicians have not tested anti-Dnajc5/Csp antibody A04220 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Dnajc5/Csp antibody A04220 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish spleen in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-19
Question
I would like to test anti-Dnajc5/Csp antibody A04220 on rat leukemic t-cell for research purposes, then I may be interested in using anti-Dnajc5/Csp antibody A04220 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Roberts
Verified customer
Asked: 2013-04-19
Answer
The products we sell, including anti-Dnajc5/Csp antibody A04220, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-04-19
