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Product Info Summary
| SKU: | A01094 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-DNAM-1 CD226 Antibody
SKU/Catalog Number
A01094
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DNAM-1 CD226 Antibody catalog # A01094. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNAM-1 CD226 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01094)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CD226/DNAM-1. AA range:287-336
Reactive Species
A01094 is reactive to CD226 in Human, Monkey, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01094 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using CD226/DNAM-1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of COS7 cells using DNAM-1 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from COS7 and K562 cells, treated with PMA 125ng/ml 30' , using CD226/DNAM-1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HUVECcells using CD226/DNAM-1 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-DNAM-1 CD226 Antibody (A01094)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-DNAM-1 CD226 Antibody
Question
Is this A01094 anti-DNAM-1 antibody reactive to the isotypes of CD226?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The immunogen of A01094 anti-DNAM-1 antibody is Synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-18
Question
Does anti-DNAM-1 antibody A01094 work on bovine WB with leukocyte?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
Our lab technicians have not validated anti-DNAM-1 antibody A01094 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-DNAM-1 antibody A01094 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-20
Question
Does A01094 anti-DNAM-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Singh
Verified customer
Asked: 2017-12-20
Answer
As indicated on the product datasheet, A01094 anti-DNAM-1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-20
Question
We are currently using anti-DNAM-1 antibody A01094 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it possible that the antibody can work on horse tissues as well?
T. Carter
Verified customer
Asked: 2016-08-11
Answer
The anti-DNAM-1 antibody (A01094) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-11
Question
Is a blocking peptide available for product anti-DNAM-1 antibody (A01094)?
E. Zhao
Verified customer
Asked: 2015-06-12
Answer
We do provide the blocking peptide for product anti-DNAM-1 antibody (A01094). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-06-12
Question
I have attached the WB image, lot number and protocol we used for platelet using anti-DNAM-1 antibody A01094. Please let me know if you require anything else.
T. Zhang
Verified customer
Asked: 2015-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-27
