Anti-Collagen I/COL1A1 Antibody Picoband®

Western blot analysis of COL1A1 using anti-COL1A1 antibody (PB9939).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat skin tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse skin tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1 antigen affinity purified polyclonal antibody (PB9939) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COL1A1 at approximately 130,180-200 kDa. The expected band size for COL1A1 is at 138 kDa.

OST treatment attenuated TiPs-induced osteogenesis inhibition in vivo. ( A ) Representative Masson’s trichrome staining images of calvarial sections. Scale bar, 200 μm (upper), 50 μm (lower). ( B ) Quantitative analysis of new bone area fraction (%) determined by Masson’s trichrome staining. ( C ) Representative images of calcein (green) and alizarin red (red) double labeling with a 10-day interval. Scale bar, 50 μm (upper), 20 μm (lower). ( D ) Quantitative analysis of average periosteum mineral apposition rates (MAR, µm/d). ( E-F ) Representative images and quantitative analysis of immunohistochemical (IHC) staining for osteocalcin (OCN). Scale bar, 100 μm (upper), 50 μm (lower). ( G-H ) Representative images and quantitative analysis of IHC staining for collagen type I alpha 1 (COL1α1). Scale bar, 100 μm (upper), 50 μm (lower). ( I-J ) Western blot analysis of the expression of osteogenic markers (OCN, COL1α1) in calvarial bone tissue samples from each group. n = 6. The selected images reflect typical examples from each group, closely representing the median degree as per statistical analysis. Data are presented as mean ± SD. One-way ANOVA with Tukey’s post hoc test. The relative COL1α1 protein levels were analyzed using Brown-Forsythe and Welch ANOVA tests followed by Tamhane T2 post hoc tests. ** P < 0.01 versus the Sham group. # P < 0.05 and ## P < 0.01 versus the TiPs group. ns , not statistically significant versus the TiPs group
Index in PubMed under a CC BY license. PMID: 39707212

Antifibrotic effect of microcystin (MC) in renal fibrosis mice. Mice were treated with MC-LR (20 μg/kg/day) or MC-RR (20 μg/kg/day) by intragastrical administration for 4 weeks in advance, and then unilateral ureteral ligation was performed to construct a mouse model of obstructive renal fibrosis. The operated mice were administrated with MC-LR or MC-RR for another week, and then were euthanized for further analysis. (A) Schematic diagram of the experimental design. Forty mice were divided into four groups, Sham, unilateral ureteral obstruction (UUO), UUO + MC-LR, and UUO + MC-RR groups, each with 10 mice. No mice died during the experiment. (B,C) Kidney tissue sections were employed for H&E and Masson staining (scale bar: 40 μm). (D) Fibrosis positive area in Masson staining were assessed by pathologist blind to this study ( n = 6). (E,F) The protein level of α -smooth muscle actin (α-SMA), fibronectin and collagen I were measured in the obstructive renal tissues of UUO mice by western blot. * p < 0.05, ** p < 0.01 determined by one-way ANOVA with S–N–K post hoc analysis. (G) Residual contents of MC were detected in the kidney tissues of model mice treatment with MC-LR or MC-RR using the ELISA method. Data were analyzed using independent Student’s t -test, * p < 0.05.
Index in PubMed under a CC BY license. PMID: 35754468

Renoprotection effect of microcystin (MC)-RR in different doses on unilateral ureteral obstruction (UUO) mice. The regimen of MC-RR was used in UUO mice. Twenty-five mice were divided into five groups, Sham, UUO, and UUO mice with treatment of MC-RR in three different doses (5, 10, and 20 μg/kg/day), each group with 5 mice. (A,B) Kidney tissue sections were employed for H&E and Masson staining (scale bar: 40 μm). (C) Fibrosis positive area in Masson staining were assessed by pathologist blind to this study ( n = 5). (D,E) Protein level of α -smooth muscle actin (α-SMA), fibronectin, and collagen I were measured in the renal tissues of UUO mice by western blot. (F,G) Protein expression of TGF-β, p-Smad3, and Smad3 in kidney tissue was measured by western blot. * p < 0.05, ** p < 0.01 determined by one-way ANOVA with S–N–K post hoc analysis.
Index in PubMed under a CC BY license. PMID: 35754468

Morphological features and identification of primary cultured tibial osteoblasts of broiler chicks. (A) Morphological image of P0 cells, day of 3 culture; (B) morphological image of P1 cells, day of 2 culture; (C) morphological image of P1 cells, day of 10 culture; (D) primary osteoblasts were positive in ALP straining; (E) immunofluorescence of COL1A1 in the primary tibial osteoblasts; (F) mineralized nodules were positive in ARS staining. ALP, alkaline phosphatase; COL1A1, collagen type I alpha 1; ARS, alizarin res S.
Index in PubMed under a CC BY license. PMID: 35392115

KD inhibits the expression of the fibrosis-related proteins in the rat model (A) The results of the IHC staining displayed the expression of α -SMA of the liver histology slices. KD significantly reduced the expression of brown stained α -SMA after irradiation (B) The IHC scores for grading the expression of α -SMA (C) Expression and localization of collagen in the liver tissue. The red stain represented collagen, which indicated that collagen expression in the IR + KD group was significantly lower than that in the IR group (D-F) The results of the western blots showed the expression of α -SMA and FN proteins in the liver tissue. The administration of KD after irradiation resulted in decreased expression of a -SMA and FN. For all results in this figure, original magnification, ×100 and ×200. Mean ± SEM. n. s. denotes not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Index in PubMed under a CC BY license. PMID: 35126163

Fluorescence images of tenocytes after 5 days of culture on the surface of a culture plate (control group) and acellular amniotic membrane (amnion group). Tenocytes presented a clear cytoskeleton, good biocompatibility with acellular amniotic membrane, and even distribution on the surface of the materials, showing better growth activity than the control group (A,B) . Cell viability was measured by CCK-8, and the proliferation curve of tenocytes in the control and amniotic membrane groups was drawn (C) . Western blot assay for collagen I, fibronectin, TGF-β1, and bFGF expression in the tenocytes of the control and amnion groups for 1 week (D,E) . * p < 0.05; ** p < 0.01; and *** p < 0.001.
Index in PubMed under a CC BY license. PMID: 32478059

Representative immunofluorescence images of collagen I. Fluorescent micrographs of tenocytes after 2 and 5 days of culture on the surface of a culture plate and acellular amniotic membrane. Tenocyte nucleus shape observed under a fluorescence microscope (A,D,G,J) . Collagen I presented positive after the fluorescent FITC mark was observed under a fluorescence microscope (B,E,H,K) . Tenocyte nucleus and collagen I merging (C,F,I,L) . The corresponding semi quantitative analysis of collagen fluorescence intensity in panels (M,N) (scale bar = 50 um, n = 5, * P < 0.05 and ** P < 0.01).
Index in PubMed under a CC BY license. PMID: 32478059

Evaluation of the effect of CGA on liver histopathological and immunohistochemistry (IHC) in liver tissue. (A) Histological images of rat livers stained with H&E (original magnification, ×200). (B) The histopathologic detection of collagen in the liver by Masson’s trichrome stain (original magnification, ×100). (C,D) Effects of CGA on α-SMA and collagen I expression were examined with immunohistochemistry in liver tissue (original magnification, ×100).
Index in PubMed under a CC BY license. PMID: 29311932

VDR deficiency exacerbated hepatic fibrosis and steatosis in mice. A mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). B mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 5). C Western blot analysis of VDR and TGFβ/Smad pathway (n = 3), D Serum levels of liver function markers (AST, ALT, ALP, and TBIL) (n = 5). * P < 0.05, ** P < 0.01 Full size image
Index in PubMed under a CC BY license. PMID: 40514735

VDR activation attenuated TGF-β1-induced fibrosteatotic changes in HSCs. A Western blot analysis of VDR and α-SMA (n = 3). B mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). C mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 6). * P < 0.05, ** P < 0.01 Full size image
Index in PubMed under a CC BY license. PMID: 40514735

SI inhibited TGF-β1-induced activation of HSCs. A Chemical structure of the SI. B Cell viability of HSCs. C mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). D Immunofluorescence analysis of α-SMA. scale bar = 20 μm. (n = 6). E Western blot analysis of α-SMA. F Molecular docking results of VDR and SI. G CETSA analysis. (n = 3) * P < 0.05, ** P < 0.01 Full size image
Index in PubMed under a CC BY license. PMID: 40514735

SI ameliorated CCl 4 -induced liver fibrosis in mice. A Serum levels of liver function markers (AST, ALT, ALP, and TBIL). B Liver tissue photograph and histopathological analysis (HE, Sirius Red, Masson), scale bar = 200 μm. C Serum calcium concentrations. D mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). (n = 5) * P < 0.05, ** P < 0.01 Full size image
Index in PubMed under a CC BY license. PMID: 40514735

SI regulated VDR and fatty acid metabolism to suppress TGFβ/Smad pathway in CCl 4 -induced mice. A Immunohistochemistry analysis of TGF-β1, p-Smad3, α-SMA, COL1 A1. scale bar = 200 μm. (n = 5). B Western blot analysis of VDR, TGF-β1, and α-SMA. C mRNA levels of Cpt1a . D mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 6) * P < 0.05, ** P < 0.01 Full size image
Index in PubMed under a CC BY license. PMID: 40514735

VDR knockout abolished the SI-induced inhibition of HSCs activation. A Western blot analysis (n = 3). B Immunofluorescence analysis of α-SMA. scale bar = 20 μm. (n = 6). C mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). (n = 6) *P < 0.05, **P < 0.01 compared with si-NC + TGF-β1; $ P < 0.05, $$ P < 0.01 compared with si-NC; # P < 0.05, ## P < 0.01 compared with si-VDR + TGF-β1 Full size image
Index in PubMed under a CC BY license. PMID: 40514735

IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939).
COL1A1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939).
COL1A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939).
COL1A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.