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Col1a1 antibody (PA2140-2) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 15 Publications

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pa2140 2 col1a1 primary antibodies if testing 1

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IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2).
Collagen I/COL1A1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Fluoro550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Collagen I/COL1A1 Antibody Picoband®

Col1a1 antibody

15 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, ICC, WB

Tested Applications

Col1a1 antibody was positively detected by WB in:

human placenta tissue, rat skin tissue, mouse skin tissue, mouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Col1a1 antibody was positively detected by IHC in:

human bladder cancer tissue, human esophageal squamous cell carcinoma tissue, human clear cell renal cell carcinoma tissue, human placenta tissue, human rectum adenocarcinoma tissue, mouse lung tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Col1a1 antibody was positively detected by IF in:

human endometrial cancer tissue, human intestinal cancer tissue, rat lung tissue

Col1a1 antibody was positively detected by ICC/IF in:

NIH/3T3 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Recommend

Tested

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Collagen I/COL1A1 Antibody Picoband®
View all COL1A1 Antibodies

Form

Lyophilized

Uniprot

P11087

Gene ID

12842

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, identical to the related rat sequence, and different from the related human sequence by two amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

130 kDa, 220 kDa, 250 kDa

Calculated Molecular Weight

138.0 kDa

Background of Col1a1

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Cite This Product

Anti-Collagen I/COL1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2140-2)

Protocols

Western Blot protocol for Col1a1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Col1a1 antibody

Review Protocol

Immunofluorescence (IF) protocol for Col1a1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Col1a1 antibody

Review Protocol

Publications

ASGR1 Deficiency Promotes Liver Fibrosis by Enhancing Pro‐Inflammatory Arachidonic Acid Metabolism and ALP Secretion

Published: 2026-08-20

Journal: Immunity Inflammation and Disease

Application: WB

Impact Factor: 3.50

Species: Mouse

PMID: 42619505

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Anti-Collagen I/COL1A1 Antibody Picoband®

Col1a1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

ASGR1 Deficiency Promotes Liver Fibrosis by Enhancing Pro‐Inflammatory Arachidonic Acid Metabolism and ALP Secretion

Low-dose urapidil mitigates renal ischemia-reperfusion injury through matrix metalloproteinase-9 inhibition and anti-inflammatory effects

Modulatory effects of 6-Gingerol on erythrocyte deformability and morphology following lower extremity skeletal muscle ischemia-reperfusion injury in rats

Matrix and cytoskeletal tension gate stretch-induced calcium signaling

Depletion of tropomyosin 1.6 alleviates TGF-β1-induced fibroblast activation by promoting the α-SMA-MMP9 matrix-degrading structure

Reversing Pathology in an Aggravated Fabry Mouse Model Using Low-Dose Engineered Human Alpha-Galactosidase A AAV Gene Therapy

Evaluation of the effects of thymoquinone on red blood cell deformability, morphology, and endothelial nitric oxide synthase (eNOS) synthesis in rat lower extremity ischemia-reperfusion injury

Developing Gene Therapy for Mitigating Multisystemic Pathology in Fabry Disease: Proof of Concept in an Aggravated Mouse Model

Molecular Interaction of Soluble Klotho with FGF23 in the Pathobiology of Aortic Valve Lesions Induced by Chronic Kidney Disease

Extracellular matrix composition affects outgrowth of dendrites and dendritic spines on cortical neurons

Efficacy of probiotic Streptococcus thermophilus in counteracting TGF-β1-induced fibrotic response in normal human dermal fibroblasts

Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity

Heparan sulfate is necessary for the early formation of nascent fibronectin and collagen I fibrils at matrix assembly sites

Food-grade titanium dioxide (E171) by solid or liquid matrix administration induces inflammation, germ cells sloughing in seminiferous tubules and blood-testis barrier disruption in mice

Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone

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