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Anti-Collagen I/COL1A1 Antibody Picoband™
Boster Bio Anti-Collagen I/COL1A1 Antibody Picoband™ catalog # PB9938. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen I/COL1A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9938)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen I (1194-1218aa AGFDFSFLPQPPQEKAHDGGRYYRA), different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9938 is reactive to COL1A1 in Human
PB9938 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Collagen I using anti-Collagen I antibody (PB9938).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I antigen affinity purified polyclonal antibody (Catalog # PB9938) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I at approximately 180 kDa. The expected band size for Collagen I is at 139 kDa.
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Figure 2. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Gene/Protein Information For COL1A1 (Source: Uniprot.Org, NCBI)
Collagen alpha-1(I) chain
fibrillar collagen family
Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on COL1A1, check out the COL1A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COL1A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PB9938 has been cited in 50 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang Y,Liu JM,Huang JP,Lu KX,Sun WL,Tan JY,Li BX,Chen LL,Wu Y. Regeneration potential of decellularized periodontal ligament cell sheets combined with 15-Deoxy-Δ12,14-prostaglandin J2nanoparticles in a rat periodontal defect. Biomed Mater.2021 Mar 12.doi:10.1088/1748-605X/abee61.Epub ahead of print.PMID:33711827.
PB9938 usage in article: APP:IHC, SAMPLE:MANDIBLES, DILUTION:1:100
Lombardi, F.; Augello, F.R.; Palumbo, P.; Mollsi, E.; Giuliani, M.; Cimini, A.M.; Cifone, M.G.; Cinque, B. Soluble Fraction from Lysate of a High Concentration Multi-Strain Probiotic Formulation Inhibits TGF-β1-Induced Intestinal Fibrosis on CCD-18Co Cells. Nutrients 2021,13,882. https://doi.org/10.3390/nu13030882
PB9938 usage in article: APP:WB, SAMPLE:HUMAN INTESTINAL FIBROBLAST CELL, DILUTION:1:1000
Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
PB9938 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:400
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
PB9938 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:1:100
Ketamine analog methoxetamine induced inflammation and dysfunction of bladder in rats
Uncarboxylated osteocalcin inhibits high glucose-induced ROS production and stimulates osteoblastic differentiation by preventing the activation of PI3K/Akt %u2026
Synthesis, Biological Evaluation, and Docking Studies of a Novel Sulfonamido-Based Gallate as Pro-Chondrogenic Agent for the Treatment of Cartilage
Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-?1
Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-?1/Smad pathway
Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-?1-Induced Epithelial-to-Mesenchymal Transition
An essential role for Wnt/?-catenin signaling in mediating hypertensive heart disease
Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction
FOXA2 alleviates CCl4-induced liver fibrosis by protecting hepatocytes in mice
Lipoxin A4 improves erectile dysfunction in rats with type I diabetes by inhibiting oxidative stress and corporal fibrosis
Screening in larval zebrafish reveals tissue-specific distribution of fifteen fluorescent compounds
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Inhibitory effects of dimethyl ?-ketoglutarate in hepatic stellate cell activation
Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target
Dietary Flavonoid Hyperoside Induces Apoptosis of Activated Human LX-2 Hepatic Stellate Cell by Suppressing Canonical NF-?B Signaling
Effects of rhBNP on myocardial fibrosis after myocardial infarction in rats
Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication
Continuing Treatment with Salvia miltiorrhiza Injection Attenuates Myocardial Fibrosis in Chronic Iron-Overloaded Mice
Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix
The Osteogenic Potential of Mesoporous Bioglasses/Silk and Non-Mesoporous Bioglasses/Silk Scaffolds in Ovariectomized Rats: In vitro and In vivo Evaluation
Effects of angiotensin II receptor antagonist, Losartan on the apoptosis, proliferation and migration of the human pancreatic stellate cells
Effects of pentoxifylline on the hepatic content of TGF-?1 and collagen in Schistosomiasis japonica mice with liver fibrosis
Estrogen reduces CCL4- induced liver fibrosis in rats
Hesperetin alleviates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro
Angiotensin II induces connective tissue growth factor expression in human hepatic stellate cells by a transforming growth factor ?-independent mechanism
Protective effects of baicalin on rabbit articular chondrocytes in vitro
The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway
Silver nanoparticles/chitosan oligosaccharide/poly(vinyl alcohol) nanofiber promotes wound healing by activating TGF?1/Smad signaling pathway
Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-?1 with concomitant induce Smad7 in human hypertrophic scar fibroblasts
Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro
Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An?In Vitro?Study
Effect of anterior cervical discectomy and fusion on adjacent segments in rabbits
Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats
Luo L, Li J, Liu H, Jian X, Zou Q, Zhao Q, Le Q, Chen H0, Gao X, He C. Int J Mol Sci. 2017 May 12;18(5). pii: E1044. doi: 10.3390/ijms18051044. Adiponectin Is Involved in Connective Tissue Growth Factor-Induced Proliferation, Migration and Overpro...
Cheng L, Ge M, Lan Z, Ma Z, Chi W, Kuang W, Sun K, Zhao X, Liu Y, Feng Y, Huang Y, Luo M, Li L, Zhang B, Hu X, Xu L, Liu X, Huo Y, Deng H, Yang J, Xi Q, Zhang Y, Siegenthaler JA, Chen L. Arch Toxicol. 2018 Jan;92(1):469-485. doi: 10.1007/s00204-01...
Huang L, Jin P, Lin X, Lin C, Zheng L, Zhao J. Mol Med Rep. 2017 Mar;15(3):1149-1156. doi: 10.3892/mmr.2017.6142. Epub 2017 Jan 24. Beneficial effects of sulfonamide-based gallates on osteoblasts in vitro
Wang X, Cao P, Liu J, Du P, Wang Z, Chen W, Liu C, Wu Y. Med Sci Monit. 2017 Jan 4;23:46-56. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts
Shang P, Liu W, Liu T, Zhang Y, Mu F, Zhu Z, Liang L, Zhai X, Ding Y, Li Y, Wen A. Sci Rep. 2016 Dec 23;6:39809. doi: 10.1038/srep39809. Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth...
Ge Ws, Wang Yj, Wu Jx, Fan Jg, Chen Yw, Zhu L. Mol Med Rep. 2014 Jun;9(6):2145-51. Doi: 10.3892/Mmr.2014.2099. Epub 2014 Apr 1. ??-Catenin Is Overexpressed In Hepatic Fibrosis And Blockage Of Wnt/??-Catenin Signaling Inhibits Hepatic Stellate Cell...
Ma X, Xu L, Wang S, Chen H, Xu J, Li X, Ning G. Lab Invest. 2009 Aug;89(8):903-14. Doi: 10.1038/Labinvest.2009.51. Epub 2009 Jun 1. Loss Of Steroid Receptor Co-Activator-3 Attenuates Carbon Tetrachloride-Induced Murine Hepatic Injury And Fibrosis.
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...
Zhu B, Xu T, Yuan J, Guo X, Liu D. Plos One. 2013 Sep 19;8(9):E76282. Doi: 10.1371/Journal.Pone.0076282. Ecollection 2013. Transcriptome Sequencing Reveals Differences Between Primary And Secondary Hair Follicle-Derived Dermal Papilla Cells Of The...
Liang J, Deng X, Lin Zx, Zhao Lc, Zhang Xl. World J Gastroenterol. 2009 Sep 28;15(36):4529-37. Attenuation Of Portal Hypertension By Natural Taurine In Rats With Liver Cirrhosis.
Ge Ws, Wu Jx, Fan Jg, Wang Yj, Chen Yw. World J Gastroenterol. 2011 Sep 28;17(36):4090-8. Doi: 10.3748/Wjg.V17.I36.4090. Inhibition Of High-Mobility Group Box 1 Expression By Sirna In Rat Hepatic Stellate Cells.
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.
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