Skip to the beginning of the images gallery

COL1A1 antibody (PB9938) performs well in IF, IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 42 Publications

pb9938 col1a1 primary antibodies if testing 9

IF analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-Collagen I/COL1A1 Antibody Picoband®

COL1A1 antibody

42 Publications

Reactive Species	Host	Application
Human	Rabbit	IF, IHC, WB

Tested Applications

COL1A1 antibody was positively detected by WB in:

human placenta tissue, rat skin tissue, rat lung tissue, mouse skin tissue, mouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

COL1A1 antibody was positively detected by IHC in:

human liver cancer tissue, human placenta tissue, human renal cell carcinoma tissue, human bladder urothelial carcinoma tissue, human lung adenocarcinoma tissue, human cervical cancer tissue, human tonsillitis tissue, human appendicitis tissue, mouse lung tissue, rat lung tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

COL1A1 antibody was positively detected by IF in:

human placenta tissue, mouse lung tissue, rat lung tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Mouse

Tested

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-Collagen I/COL1A1 Antibody Picoband®
View all COL1A1 Antibodies

Form

Lyophilized

Uniprot

P02452

Gene ID

1277

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen I, different from the related mouse and rat sequences by four amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

130-180 kDa

Calculated Molecular Weight

138.9 kDa

Background of COL1A1

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Cite This Product

Anti-Collagen I/COL1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9938)

Protocols

Western Blot protocol for COL1A1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for COL1A1 antibody

Review Protocol

Immunofluorescence (IF) protocol for COL1A1 antibody

Review Protocol

Publications

p21 Inhibition Rescues Radiation-Induced Osteogenic Dysfunction and Promotes Bone Regeneration in Osteoradionecrosis of the Jaw via the Rb–Smad2/3–Runx2 Axis

Published: 2026-08-18

Journal: ORAL DISEASES

Application: IF

Impact Factor: 3.10

Species: Mouse

PMID: 42612232

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-Collagen I/COL1A1 Antibody Picoband®

COL1A1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

p21 Inhibition Rescues Radiation-Induced Osteogenic Dysfunction and Promotes Bone Regeneration in Osteoradionecrosis of the Jaw via the Rb–Smad2/3–Runx2 Axis

LncRNA ANRIL inhibits TGF-β1-induced proliferation, migration, and fibrosis of Tenon’s capsule fibroblasts from glaucoma patients by reducing IL-22-RA1 stability and expression

Targeting CD30L Alleviates Airway Remodeling via JNK/p38 MAPK Pathway in OVA-Induced Asthmatic Mice

Exercise-induced musclin enhances efferocytosis via metabolic reprogramming to alleviate periprosthetic joint infection

NSUN2-mediated GATM m5C methylation regulates creatine metabolism to drive mitochondrial fission and promote renal fibrosis

Dickkopf-1 promotes tumor progression of gefitinib- resistant non-small cell lung cancer through cancer cell-fibroblast interactions

Renal tubular S100A7a impairs fatty acid oxidation and exacerbates renal fibrosis via both intracellular and extracellular pathway

Development of Biomimetic Substrates for Limbal Epithelial Stem Cells Using Collagen-Based Films, Hyaluronic Acid, Immortalized Cells, and Macromolecular Crowding

The deleterious effects of CDK4/6 inhibition on renal recovery post-acute kidney injury

Investigating the role of various extracellular matrix in androgenetic alopecia: Insights from immunostaining and quantitative analysis – A pilot study

Efficacy of an Innovative Poly-Component Formulation in Counteracting Human Dermal Fibroblast Aging by Influencing Oxidative and Inflammatory Pathways

Calcipotriol abrogates TGF-β1/pSmad3-mediated collagen 1 synthesis in pancreatic stellate cells by downregulating RUNX1

Evaluation of the Antifibrotic Effects of Drugs Commonly Used in Inflammatory Intestinal Diseases on In Vitro Intestinal Cellular Models

Estrogen alleviates liver fibrosis and restores metabolic homeostasis in ovariectomy-induced liver injury and carbon tetrachloride (CCl4) exposure

Harmine inhibits pulmonary fibrosis through regulating DNA damage repair-related genes and activation of TP53-Gadd45α pathway

Exposure to micron-grade silica particles triggers pulmonary fibrosis through cell-to-cell delivery of exosomal miR-107

Bosutinib Laden Three-Dimensional Printed Zein Scaffolds Promote Osteogenesis

Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin

RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway

MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88

Expression of Collagen (Types I, III, and V), HSP47, MMP-2, and TIMP-1 in Retrobulbar Adipose Tissue of Patients with Thyroid-Associated Orbitopathy

Surface modification of titanium implants by ZIF-8@Levo/LBL coating for inhibition of bacterial-associated infection and enhancement of in vivo osseointegration

Rapamycin attenuates the paraquat-induced pulmonary fibrosis through activating Nrf2 pathway

TiO2 nanotubes promote osteogenic differentiation of mesenchymal stem cells via regulation of lncRNA CCL3-AS

Micheliolide ameliorates renal fibrosis by suppressing the Mtdh/BMP/MAPK pathway

Platelet-rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF-β/SMAD signaling pathway

CDH11 promotes liver fibrosis via activation of hepatic stellate cells

Biocompatibility of injectable hydrogel from decellularized human adipose tissue in vitro and in vivo

Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels

Design, Synthesis, and Antifibrosis Activity in Liver of Nonsecosteroidal Vitamin D Receptor Agonists with Phenyl-pyrrolyl Pentane Skeleton

YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis

Role of NADPH oxidase pathway in renal protection induced by procyanidin B2: In L-NAME induced rat hypertension model

Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo

Angiotensin II induces connective tissue growth factor expression in human hepatic stellate cells by a transforming growth factor β-independent mechanism

Effect of NGF From Venom of Chinese Cobra (Naja Atra)on Chondrocytes Proliferation and Metabolism In Vitro

LncRNA-ATB/microRNA-200a/β-catenin regulatory axis involved in the progression of HCV-related hepatic fibrosis

Demineralized bone matrix used for direct pulp capping in rats

Allicin inhibits tubular epithelial‑myofibroblast transdifferentiation under high glucose conditions in vitro

RETRACTED ARTICLE: Epigallocatechin-3-gallate (EGCG) as a pro-osteogenic agent to enhance osteogenic differentiation of mesenchymal stem cells from human bone marrow: an in vitro study

Possible Involvement of Fibrocytes in Atrial Fibrosis in Patients With Chronic Atrial Fibrillation

Quantitative determination of matrix Gla protein (MGP) and BMP-2 during the osteogenic differentiation of human periodontal ligament cells

Inhibitory effects of local pretreated epidermis on wound scarring: A feasible method to minimize surgical scars

Review

Have you used Anti-Collagen I/COL1A1 Antibody Picoband®?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality