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Product Info Summary
| SKU: | B00329 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated
SKU/Catalog Number
B00329
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated (Catalog # B00329). Tested in Dot blot, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
Cite This Product
Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B00329)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Collagen Type I from human and bovine placenta.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
B00329 is reactive to COL1A1 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
138.9 kDa
Background of COL1A1
COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
B00329 is guaranteed for ELISA, Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:3,000 - 1:6,000
Flow Cytometry: User optimized
Fluorochrome Protein Value: 10-20
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:3,000 - 1:6,000
Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Validation Images & Assay Conditions
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Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody (600-406-103). Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.
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Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody (600-406-103) at 5µg/mL for 45 min at 4°C. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT. Courtesy of D. Figueroa NIH.
Specific Publications For Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated (B00329)
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16 Customer Q&As for Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated
Question
My boss were satisfied with the WB result of your anti-COLLAGEN Type I antibody Biotin Conjugated. However we have seen positive staining in skin secreted using this antibody. Is that expected? Could you tell me where is COL1A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From literature, skin does express COL1A1. Generally COL1A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2020-04-28
Question
Is a blocking peptide available for product anti-COLLAGEN Type I antibody Biotin Conjugated (B00329)?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We do provide the blocking peptide for product anti-COLLAGEN Type I antibody Biotin Conjugated (B00329). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-21
Question
I was wanting to use your anti-COLLAGEN Type I antibody Biotin Conjugated for IP for rat skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat skin identification?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
It shows on the product datasheet, B00329 anti-COLLAGEN Type I antibody Biotin Conjugated has been validated for Flow Cytometry, IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-14
Question
We are currently using anti-COLLAGEN Type I antibody Biotin Conjugated B00329 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-COLLAGEN Type I antibody Biotin Conjugated (B00329) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
Please see the WB image, lot number and protocol we used for skin using anti-COLLAGEN Type I antibody Biotin Conjugated B00329. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
Can you help my question with product B00329, anti-COLLAGEN Type I antibody Biotin Conjugated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Taylor
Verified customer
Asked: 2019-09-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free B00329 anti-COLLAGEN Type I antibody Biotin Conjugated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
Is this B00329 anti-COLLAGEN Type I antibody Biotin Conjugated reactive to the isotypes of COL1A1?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
The immunogen of B00329 anti-COLLAGEN Type I antibody Biotin Conjugated is Collagen Type I from human and bovine placenta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-COLLAGEN Type I antibody Biotin Conjugated B00329. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-25
Question
We have seen staining in human liver. Any tips? Is anti-COLLAGEN Type I antibody Biotin Conjugated supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
Based on literature liver does express COL1A1. Based on Uniprot.org, COL1A1 is expressed in spinal cord, spleen, brain, skin, fetal brain cortex, bone, liver, among other tissues. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2018-08-07
Question
Will B00329 anti-COLLAGEN Type I antibody Biotin Conjugated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Johnson
Verified customer
Asked: 2018-03-05
Answer
You can see on the product datasheet, B00329 anti-COLLAGEN Type I antibody Biotin Conjugated as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-05
Question
We are interested in using your anti-COLLAGEN Type I antibody Biotin Conjugated for immunoregulatory interactions between a lymphoid and a non-lymphoid cell studies. Has this antibody been tested with western blotting on mouse lung? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
We appreciate your inquiry. This B00329 anti-COLLAGEN Type I antibody Biotin Conjugated is tested on hepatic stellate cells, mouse lung, lung cells, dermal fibroblast cells. It is guaranteed to work for Flow Cytometry, IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-10
Question
you antibody to test anti-COLLAGEN Type I antibody Biotin Conjugated B00329 on rat skin for research purposes, then I may be interested in using anti-COLLAGEN Type I antibody Biotin Conjugated B00329 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Krishna
Verified customer
Asked: 2017-05-02
Answer
The products we sell, including anti-COLLAGEN Type I antibody Biotin Conjugated B00329, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-02
Question
We have tried in the past anti-COLLAGEN Type I antibody Biotin Conjugated for IHC on spinal cord a few months ago. I am using mouse, and We want to use the antibody for WB next. We are interested in examining spinal cord as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
D. Parker
Verified customer
Asked: 2017-03-30
Answer
I have checked the website and datasheets of our anti-COLLAGEN Type I antibody Biotin Conjugated and it seems that B00329 has been validated on mouse in both IHC and WB. Thus B00329 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-03-30
Question
I see that the anti-COLLAGEN Type I antibody Biotin Conjugated B00329 works with IP, what is the protocol used to produce the result images on the product page?
H. Yang
Verified customer
Asked: 2014-08-14
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-08-14
Question
Does anti-COLLAGEN Type I antibody Biotin Conjugated B00329 work for IP with skin?
R. Parker
Verified customer
Asked: 2014-06-18
Answer
According to the expression profile of skin, COL1A1 is highly expressed in skin. So, it is likely that anti-COLLAGEN Type I antibody Biotin Conjugated B00329 will work for IP with skin.
Boster Scientific Support
Answered: 2014-06-18
Question
Is there a BSA free version of anti-COLLAGEN Type I antibody Biotin Conjugated B00329 available?
K. Miller
Verified customer
Asked: 2013-09-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-COLLAGEN Type I antibody Biotin Conjugated B00329 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-09-25
