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Product Info Summary
| SKU: | M00378-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-EGF Mouse Monoclonal Antibody [Clone ID: OTI2F9]
SKU/Catalog Number
M00378-1
Size
100 µl
Description
Boster Bio EGF mouse monoclonal antibody,clone OTI2F9. Catalog# M00378-1. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGF Mouse Monoclonal Antibody [Clone ID: OTI2F9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00378-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2F9
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 1054-1207 of human EGF (NP_001954) produced in E.coli.
Reactive Species
M00378-1 is reactive to EGF in Human
Calculated molecular weight
134.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00378-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:150
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGF (1:2000).
Specific Publications For Anti-EGF Mouse Monoclonal Antibody [Clone ID: OTI2F9] (M00378-1)
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