Anti-Cdk9 Antibody Picoband™

Boster Bio Anti-Cdk9 Antibody Picoband™ catalog # PB9537. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PB9537
Size: 100μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Cdk9 Antibody Picoband™

See all CDK9 products

SKU/Catalog Number

PB9537

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cdk9 Antibody Picoband™ catalog # PB9537. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cdk9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9537)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Cdk9 recombinant protein (Position: H141-F372). Human Cdk9 shares 99.1% amino acid (aa) sequence identity with both mouse and rat Cdk9.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9537 is reactive to CDK9 in Human, Rat

Applications

PB9537 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDK9 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50750

Gene ID

1025

Gene Name

CDK9

Full Name

Cyclin-dependent kinase 9

Weight

42.778kDa

Superfamily

protein kinase superfamily

Alternative Names

C-2k; CDC2L4CTK1; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); cyclin-dependent kinase 9; EC 2.7.11; EC 2.7.11.22; EC 2.7.11.23; PITALRE; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK CDK9 C-2k, CDC2L4, CTK1, PITALRE, TAK cyclin dependent kinase 9 cyclin-dependent kinase 9|CDC2-related kinase|cell division cycle 2-like protein kinase 4|cell division protein kinase 9|serine/threonine protein kinase PITALRE|tat-associated kinase complex catalytic subunit

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDK9, check out the CDK9 Infographic

CDK9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDK9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-Cdk9 Antibody Picoband™

Question

We were content with the WB result of your anti-Cdk9 antibody. However we have observed positive staining in cervix carcinoma pml body. using this antibody. Is that expected? Could you tell me where is CDK9 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

According to literature, cervix carcinoma does express CDK9. Generally CDK9 expresses in nucleus. cytoplasm. nucleus, pml body. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-08-23

Question

We are currently using anti-Cdk9 antibody PB9537 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The anti-Cdk9 antibody (PB9537) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-19

Question

I was wanting to use using your anti-Cdk9 antibody for formation of hiv-1 elongation complex containing hiv-1 tat studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We appreciate your inquiry. This PB9537 anti-Cdk9 antibody is tested on jurkat whole cell lysate, rat intestine tissue, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-09

Question

We ordered your anti-Cdk9 antibody for IHC on cervix carcinoma a few years ago. I am using human, and We want to use the antibody for WB next. I am interested in examining cervix carcinoma as well as cervix in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

I took a look at the website and datasheets of our anti-Cdk9 antibody and I see that PB9537 has been tested on human in both IHC and WB. Thus PB9537 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-26

Question

We have seen staining in human cervix. What should we do? Is anti-Cdk9 antibody supposed to stain cervix positively?

S. Johnson

Verified customer

Asked: 2016-12-14

Answer

Based on literature cervix does express CDK9. Based on Uniprot.org, CDK9 is expressed in right ovary, cervix, cervix carcinoma, liver, among other tissues. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2016-12-14

Conjugation

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PB9537
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