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Product Info Summary
| SKU: | PB9537 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cdk9 Antibody Picoband®
SKU/Catalog Number
PB9537
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cdk9 Antibody Picoband® catalog # PB9537. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdk9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9537)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cdk9 recombinant protein (Position: H141-F372). Human Cdk9 shares 99.1% amino acid (aa) sequence identity with both mouse and rat Cdk9.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9537 is reactive to CDK9 in Human, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.8 kDa
Background of CDK9
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9537 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT Whole Cell
IHC: rat intestine tissue, human intestinal cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Cdk9 using anti-Cdk9 antibody (PB9537).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: JURKAT Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk9 antigen affinity purified polyclonal antibody (Catalog # PB9537) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk9 at approximately 43 kDa. The expected band size for Cdk9 is at 43 kDa.
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IHC analysis of Cdk9 using anti-Cdk9 antibody (PB9537).
Cdk9 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdk9 Antibody (PB9537) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of Cdk9 using anti-Cdk9 antibody (PB9537).
Cdk9 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdk9 Antibody (PB9537) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Cdk9 Antibody Picoband® (PB9537)
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5 Customer Q&As for Anti-Cdk9 Antibody Picoband®
Question
We were content with the WB result of your anti-Cdk9 antibody. However we have observed positive staining in cervix carcinoma pml body. using this antibody. Is that expected? Could you tell me where is CDK9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
According to literature, cervix carcinoma does express CDK9. Generally CDK9 expresses in nucleus. cytoplasm. nucleus, pml body. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-08-23
Question
We are currently using anti-Cdk9 antibody PB9537 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The anti-Cdk9 antibody (PB9537) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-19
Question
I was wanting to use using your anti-Cdk9 antibody for formation of hiv-1 elongation complex containing hiv-1 tat studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We appreciate your inquiry. This PB9537 anti-Cdk9 antibody is tested on jurkat whole cell lysate, rat intestine tissue, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-09
Question
We ordered your anti-Cdk9 antibody for IHC on cervix carcinoma a few years ago. I am using human, and We want to use the antibody for WB next. I am interested in examining cervix carcinoma as well as cervix in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
I took a look at the website and datasheets of our anti-Cdk9 antibody and I see that PB9537 has been tested on human in both IHC and WB. Thus PB9537 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-07-26
Question
We have seen staining in human cervix. What should we do? Is anti-Cdk9 antibody supposed to stain cervix positively?
S. Johnson
Verified customer
Asked: 2016-12-14
Answer
Based on literature cervix does express CDK9. Based on Uniprot.org, CDK9 is expressed in right ovary, cervix, cervix carcinoma, liver, among other tissues. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2016-12-14
