Overview
| Product Name |
Rat EGF ELISA Kit PicoKine™
See more Egf products |
| Catalog# |
EK0954 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Rat EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.. Sensitivity: 1pg/ml. |
| Cite This Product |
Rat EGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0954)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
| Sensitivity |
<1 pg/ml |
| Assay Range |
7.8 pg/ml - 500 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: N974-R1026 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Egf (Source: Uniprot.org, NCBI)
| Uniprot Id | P07522 |
|---|
| NCBI Gene Id | 25313 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Egf |
|---|
| Protein Name | Pro-epidermal growth factor |
|---|
| Alternative Names | Egf|beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone |
|---|
| Molecular Weight | 124126 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Egf, check out the Egf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Egf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Egf infographic
Kit Components And Assay QC Data Details Of Rat EGF ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0954-CAP |
Anti-Rat Egf Pre-coated 96-well strip microplate | 1 |
| EK0954-ST | Rat Egf Standard | 2 vials, 10 ng/tube |
| EK0954-DA | Rat Egf Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0954 Rat EGF ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.033 | 0.129 | 0.207 | 0.406 | 0.715 | 1.210 | 2.012 | 2.550 |
We measured random samples of Rat EGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 11 | 90 | 232 | 10 | 96 | 211 |
| Standard deviation | 0.75 | 5.31 | 16.24 | 0.83 | 6.91 | 16.66 |
| CV (%) | 6.9% | 5.9% | 7% | 8.3% | 7.2% | 7.9% |
We measured the performance consistency of Rat EGF ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 11 | 12 | 11 | 11 | 11 | 0.43 | 3.9% |
| Sample 2 | 90 | 86 | 103 | 93 | 93 | 6.28 | 6.7% |
| Sample 3 | 232 | 239 | 257 | 243 | 242 | 9.12 | 3.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 4
| Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y. Stem Cell Res Ther. 2017 Nov 21;8(1):267. doi: 10.1186/s13287-017-0718-8. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats |
PubMed ID 29157289 |
| Li, J.J., Wu, X.Y., Chen, J.L., Chen, G.R., Xu, J., Gu, Y., & Song, H.P. (2017). Antiplatelet drug ticagrelor delays gastric ulcer healing in rats. Experimental and Therapeutic Medicine,14(4), 3774-3779. doi: 10.3892/etm.2017.4955 |
PubMed ID 29042978 |
| Russo F, Linsalata M, Clemente C, D'Attoma B, Orlando A, Campanella G, Giotta F, Riezzo G. Bmc Cancer. 2013 Feb 4;13:56. Doi: 10.1186/1471-2407-13-56. The Effects Of Fluorouracil, Epirubicin, And Cyclophosphamide (Fec60) On The Intestinal Barrier ... |
PubMed ID 23379680 |
| Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ... |
PubMed ID 19005168 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0954 Rat EGF ELISA Kit PicoKine™
8 Related Questions
Question
Q: can you recommend the dilution ratio of serum samples for detection of EGF in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Asked: 2019-12-31
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the EGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-12-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2019-07-19
Boster Scientific Support
Answered: 2019-07-19
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
S. Mitchell
Asked: 2019-05-27
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-27
Question
Q: can I use citrate plasma as samples in Rat EGF Picokine® ELISA Kit (Catalog # EK0954)?
Verified Customer
Asked: 2018-07-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of EGF causing degradation of its protein structure. EGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the EGF ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-07-17
Question
Q: how do I analyze ELISA data? I have obtained EGF level in serum.
Verified Customer
Asked: 2018-04-07
Boster Scientific Support
Answered: 2018-04-07
Question
Q: if the enzyme conjugated EGF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the EGF antigen?
Verified Customer
Asked: 2017-10-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-10-21
Question
Q: which procedure should I follow in order to thaw whole blood sample for EGF ELISA after freezing?
Verified Customer
Asked: 2017-05-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test EGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-05-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2016-01-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-01-14
