Product Info Summary
SKU: | A02578-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NR4A3/Nor1 Antibody
SKU/Catalog Number
A02578-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NR4A3/Nor1 Antibody (Catalog# A02578-1). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NR4A3/Nor1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02578-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NR4A3. Immunogen sequence location: 387-436
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02578-1 is reactive to NR4A3 in Human, Mouse, Rat
Applications
A02578-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
68.23kDa
Background of NR4A3
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from COLO cells, using NR4A3 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For NR4A3 (Source: Uniprot.org, NCBI)
Gene Name
NR4A3
Full Name
Nuclear receptor subfamily 4 group A member 3
Weight
68.23kDa
Superfamily
nuclear hormone receptor family
Alternative Names
CHN; CSMF; MINOR; NOR1; TEC NR4A3 CHN, CSMF, MINOR, NOR1 nuclear receptor subfamily 4 group A member 3 nuclear receptor subfamily 4 group A member 3|chondrosarcoma, extraskeletal myxoid, fused to EWS|mitogen-induced nuclear orphan receptor|neuron-derived orphan receptor 1|nuclear hormone receptor NOR-1|translocated in extraskeletal chondrosarcoma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR4A3, check out the NR4A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR4A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR4A3/Nor1 Antibody (A02578-1)
Hello CJ!
No publications found for A02578-1
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-NR4A3/Nor1 Antibody
Question
We are currently using anti-NR4A3/Nor1 antibody A02578-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
L. Johnson
Verified customer
Asked: 2014-02-07
Answer
The anti-NR4A3/Nor1 antibody (A02578-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-02-07