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- Table of Contents
GFRA1 antibody (PB9202) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of GFRA1 using anti-GFRA1 antibody (PB9202).
GFRA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GFRA1 Antibody (PB9202) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
GFRA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GFRA1 Antibody (PB9202) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband®
GFRA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
GFRA1 antibody was positively detected by WB in:
human placenta tissue,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GFRA1 antibody was positively detected by IHC in:
human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband®
View all GFRA1 Antibodies
View all GFRA1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated Molecular Weight
51.5 kDa
Background of GFRA1
GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor or GFRA1, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to chromosome 10q25.3. The protein encoded by this gene is a member of the GDNF receptor family. GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is also a candidate gene for Hirschsprung disease. Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase.
Cite This Product
Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9202)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
98%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Rat
97%
High Homology
Mouse
97%
High Homology
Dog
97%
High Homology
Zebrafish
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GFRA1 antibody
Immunohistochemistry (IHC) protocol for GFRA1 antibody
Publications
Application:
Species:
Construction and Performance Evaluation of an Astaxanthin–Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells
Published: 2025-11-24
Journal: Biology-Basel
Application: IF
Impact Factor: 3.50
Species: Mouse
JMJD3 regulate H3K27me3 modification via interacting directly with TET1 to affect spermatogonia self-renewal and proliferation
Published: 2024-02-29
Journal: BMC GENOMICS
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 38424516
Epigenetic Regulation of TET1-SP1 During Spermatogonia Self-Renewal and Proliferation
Published: 2022-02-11
Journal: Frontiers in Physiology
Application: WB
Impact Factor: 4.76
Species: Mouse
PMID: 35222097
Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues
Published: 2021-05-11
Journal: CRYOBIOLOGY
Application: IF
Impact Factor: 2.49
Species: Bovine
PMID: 33989617
Cryopreservation of calf testicular tissues with knockout serum replacement
Published: 2020-01-16
Journal: CRYOBIOLOGY
Application: IHC
Impact Factor: 2.29
Species: Calf
PMID: 31954710
Enrichment and in vitro features of the putative gonocytes from cryopreserved testicular tissue of neonatal bulls
Published: 2016-07-01
Journal: Andrology
Application: IHC,ICC
Impact Factor: 2.52
Species: Bovine
PMID: 27371507
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Product Promise
We promise Anti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9202
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026