Anti-GFR alpha 1 GFRA1 Antibody

Product Name

Anti-GFR alpha 1 GFRA1 Antibody

View all GFRA1 Antibodies

SKU/Catalog Number

A02897

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GFR alpha 1 GFRA1 Antibody (Catalog # A02897). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GFR alpha 1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GFR alpha 1 GFRA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02897)

Host

Rabbit

Contents

GFR alpha 1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GFR alpha 1 antibody was raised against a 14 amino acid peptide near the center of human GFR alpha 1. The immunogen is located within amino acids 360 - 410 of GFR alpha 1.

Cross-reactivity

GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins.

Reactive Species

A02897 is reactive to GFRA1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

51.5 kDa

Background of GFRA1

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently. GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02897 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GFRA1

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