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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine™ (96 Tests). Quantitate Human GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0362)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: R109-I211
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0362 is reactive to GDNF in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0362 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0362-CAP||Anti-Human GDNF Pre-coated 96-well strip microplate||1|
|EK0362-ST||Human GDNF Standard||2 vials, 10 ng/tube|
|EK0362-DA||Human GDNF Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human GDNF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of GDNF in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For GDNF (Source: Uniprot.Org, NCBI)
Glial cell line-derived neurotrophic factor
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3 GDNF ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 glial cell derived neurotrophic factor glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on GDNF, check out the GDNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine™ (EK0362)
EK0362 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ahmed MN,Shi D,Dailey MT,Rothermund K,Drewry MD,Calabrese TC,Cui XT,Syed-Picard FN. Dental pulp cell sheets enhance facial nerve regeneration via local neurotrophic factor delivery. Tissue Eng Part A.2020 Nov 9. doi:10.1089/ten.TEA.2020.0265.Epub ahead of
Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells
Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.
Yang C, Zhou L, Gao X, Chen B, Tu J, Sun H, Liu X, He J, Liu J, Yuan Q. Neurosurgery. 2011 Mar;68(3):691-704. Doi: 10.1227/Neu.0B013E3182098A8A. Neuroprotective Effects Of Bone Marrow Stem Cells Overexpressing Glial Cell Line-Derived Neurotrophic ...
Liu G, Wang X, Shao G, Liu Q. Mol Med Rep. 2014 Apr;9(4):1305-12. Doi: 10.3892/Mmr.2014.1963. Epub 2014 Feb 18. Genetically Modified Schwann Cells Producing Glial Cell Line-Derived Neurotrophic Factor Inhibit Neuronal Apoptosis In Rat Spinal Cord ...
Chai L, Guo H, Li H, Wang S, Wang Yl, Shi F, Hu Lm, Liu Y, Adah D. J Ethnopharmacol. 2013 Oct 28;150(1):100-7. Doi: 10.1016/J.Jep.2013.08.011. Epub 2013 Sep 4. Scutellarin And Caffeic Acid Ester Fraction, Active Components Of Dengzhanxixin Injecti...
Banerjee A, N??rnberger S, Hennerbichler S, Riedl S, Schuh Cm, Hacobian A, Teuschl A, Eibl J, Redl H, Wolbank S. Cell Tissue Bank. 2014 Jun;15(2):227-39. Doi: 10.1007/S10561-013-9401-1. Epub 2013 Oct 29. In Toto Differentiation Of Human Amniotic M...
Wang X, Hou Z, Yuan Y, Hou G, Liu Y, Li H, Zhang Z. J Affect Disord. 2011 Aug;132(3):418-21. Doi: 10.1016/J.Jad.2011.03.043. Epub 2011 Apr 27. Association Study Between Plasma Gdnf And Cognitive Function In Late-Onset Depression.
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