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- Table of Contents
HSPA5 Antibodies
AND HSPA5 ELISA kits, HSPA5 Proteins
Many biological assays use antibodies to detect HSPA5, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with HSPA5 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop HSPA5 antibodies...
Designing a Western blot for HSPA5? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the HSPA5 Western Blot GuideHSPA5 Infographic by Boster Bio
All HSPA5 Products
Product
Figures
Specificity
Applications
Price
Anti-GRP78 BiP/HSPA5 Antibody Picoband®
17 Citations
Human, Mouse, Rat
IHC, WB
$370
Cat # PA1815
100 μg/vial
Cat # PA1815
100 μg/vial
Anti-GRP78 BiP/HSPA5 Antibody Picoband®
8 Citations
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # PB9640
100 μg/vial
Cat # PB9640
100 μg/vial
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
1 Citations
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # M00955
100 μl
Cat # M00955
100 μl
Human, Mouse, Rat
IHC, WB
$370
Cat # M00955-1
100 μl
Cat # M00955-1
100 μl
Human, Mouse, Rat
ELISA, IP, IF, IHC, WB
$146
Cat # M00955-4
20μL, 40μL, 100μL, 200μL
Cat # M00955-4
20μL, 40μL, 100μL, 200μL
HSPA5 Overview
Facts about Endoplasmic reticulum chaperone BiP.
Endoplasmic reticulum chaperone BiP (HSPA5)
Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957).
In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal area of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).
Gene Information
| Human | |
|---|---|
| Gene Name: | HSPA5 |
| Uniprot: | P11021 |
| Entrez: | 3309 |
Family
| Belongs to: |
|---|
| heat shock protein 70 family |
Alternative Names
78 kDa glucose-regulated protein; BIP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; FLJ26106; GRP78; GRP-78; GRP78BIP; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSP70-5; HSPA5; Immunoglobulin heavy chain-binding protein; MIF2
Molecular Weight
Mass (kDA):
72.333 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 9q33.3 |
| Sequence: | 9; NC_000009.12 (125234853..125241343, complement) |
Subcellular Localizations
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Diseases
- Endoplasmic Reticulum Stress
- Physiological Stress
- Ischemia
- Diabetes Mellitus
- Reperfusion Injury
- Inflammation
- Neoplasms
- Malignant Neoplasms
- Infarction
- Kidney Diseases
- Neurodegenerative Disorders
- Diabetes Mellitus, Non-insulin-dependent
Interacting Proteins
- AKT1
- ATF6
- Cnpy2
- DMKN
- DNAJB11
- Dnajc3
- EIF2AK3
- Eif2ak3
- ERN1
- HSP90B1
Pathways
- Cell Death
- Pathogenesis
- Secretion
- Protein Folding
- Translation
- Neuroprotection
- Autophagy
- Cell Proliferation
- Fertilization
- Rna Interference
- Glycosylation
- Aging
- Proteolysis
PTMs
- Phosphorylation
- Cleavage
- Dephosphorylation
- Glycosylation
- Oxidation
Research Areas
- Cancer
- Cellular Markers
- ER Markers
- Lipid and Metabolism
- Unfolded Protein Response
- Unfolded Protein Response (UPR)
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HSPA5
bosterbio.com/bosterbio-gene-info-cards/HSPA5
References
PMID: 2840249 by Ting J., et al. Human gene encoding the 78,000-dalton glucose-regulated protein and its pseudogene: structure, conservation, and regulation.
PMID: 1480470 by Chao C.C.K., et al. A direct-repeat sequence of the human BiP gene is required for A23187-mediated inducibility and an inducible nuclear factor binding.
9 Publications of Boster HSPA5 Antibodies & ELISA Kits
- PubMed ID: 10.3892/mmr.2019.10175, Effect of endoplasmic reticulum stress involved in manganese‑induced neurotoxi...
- PubMed ID: 31500865, Chi L,Jiao D,Nan G,Yuan H,Shen J,Gao Y.miR-9-5p attenuates ischemic stroke throu...
- PubMed ID: 33819629, Huang Y,Zhao C,Kong Y,Tan P,Liu S,Liu Y,Zeng F,Yuan Y,Zhao B,Wang J.Elucidation ...
- PubMed ID: -, Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun ...
- PubMed ID: 30315526, Catalpol Inhibits Homocysteine-induced Oxidation and Inflammation via Inhibiting...
- PubMed ID: 27738755, Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reti...
- PubMed ID: 25878598, Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippoca...
- PubMed ID: 21625440, Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Ch...
- PubMed ID: 25695617, Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.201...
*More publications can be found for each product on its corresponding product page