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Hmox1 antibody (PB9085) performs well in IHC, WB and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 41 Publications

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pb9085 hmox1 primary antibodies ihc testing 1

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IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9085).
HMOX1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®

Name: Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®
Brand: Boster Bio
SKU: PB9085
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

Hmox1 antibody

41 Publications|

1 Review

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Reactive Species	Host	Application
Mouse, Rat	Rabbit	IHC, WB

Tested Applications

Hmox1 antibody was positively detected by WB in:

rat spleen tissue, rat liver tissue, rat PC-12 whole cell, mouse spleen tissue, mouse NIH/3T3 whole cell, mouse RAW264.7 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Hmox1 antibody was positively detected by IHC in:

mouse liver tissue, rat alcoholic hepatitis tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®
View all HMOX1 Antibodies

Form

Lyophilized

Uniprot

P14901

Gene ID

15368

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse HMOX1 recombinant protein (Position: E2-T261). Mouse HMOX1 shares 82% and 93% amino acid (aa) sequences identity with human and rat HMOX1, respectively.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

33 kDa

Calculated Molecular Weight

32.9 kDa

Background of Hmox1

HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.

Cite This Product

Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9085)

Protocols

Western Blot protocol for Hmox1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Hmox1 antibody

Review Protocol

Publications

PLGA nanoparticles encapsulating fibroblast growth factor 21 alleviate acetaminophen-induced acute liver injury

Published: 2026-03-16

Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY

Application: WB

Impact Factor: 3.10

Species: Mouse

PMID: 41838132

SKU	PB9085
Application	Western Blot
Sample	Liver tissue from an ANIT-induced rat model
Sample Processing Description	Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation.
Other Reagents	Blocking buffer, RIPA lysis buffer
Primary Antibody	Heme Oxygenase 1/HMOX1 Antibody Picoband®
Primary Incubation	1:2,000 dilution, incubated overnight at 4°C.
Secondary Antibody	HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation	1:50000
Detection	ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging
Results Summary	The image shows the following lanes: Lane 1 represents the liver of control group rats, displaying a clear and uniform HO-1/HMOX1 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, where the HO-1/HMOX1 band is markedly reduced. Expression of the internal control GAPDH remains consistent. These results demonstrate that the antibody effectively detects changes in HO-1/HMOX1 expression.

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Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®

Hmox1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

PLGA nanoparticles encapsulating fibroblast growth factor 21 alleviate acetaminophen-induced acute liver injury

Network Pharmacology and Untargeted Metabolomics Analysis of the Protective Mechanisms of Total Flavonoids from Chuju in Myocardial Ischemia-Reperfusion Injury

Muscone attenuates myocardial infarction by regulating ferroptosis of cardiomyocytes through Nrf2/system Xc-/GPX4 signaling pathway

GGT and GSH-triggered nanoplatform for efficient gambogic acid delivery and tumor penetration in triple-negative breast cancer

SR-B1 deficiency suppresses progression in acute myeloid leukemia via ferroptosis and reverses resistance to venetoclax

Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

Baicalein attenuates oxidative damage in mice haematopoietic cells through regulation of PDGFRβ

Daphnetin protects neurons in an Alzheimer disease mouse model and normal rat neurons by inhibiting BACE1 activity and activating the Nrf2/HO-1 pathway

Yunvjian decoction mitigates hyperglycemia in rats induced by a high-fat diet and streptozotocin via reducing oxidative stress in pancreatic beta cells

RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway

Water Extract of Rice False Smut Balls Activates Nrf2/HO-1 and Apoptosis Pathways, Causing Liver Injury

Chronic intermittent hypobaric hypoxia ameliorates osteoporosis after spinal cord injury through balancing osteoblast and osteoclast activities in rats

Untargeted serum and liver metabolomics analyses reveal the gastroprotective effect of polysaccharide from Evodiae fructus on ethanol-induced gastric ulcer in mice

Bacterial Metabolite Reuterin Attenuated LPS-Induced Oxidative Stress and Inflammation Response in HD11 Macrophages

Study on the Mechanism of Mesaconitine-Induced Hepatotoxicity in Rats Based on Metabonomics and Toxicology Network

Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway

Activation of AhR-NQO1 Signaling Pathway Protects Against Alcohol-Induced Liver Injury by Improving Redox Balance

Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway

Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus

Pterostilbene Exerts Hepatoprotective Effects through Ameliorating LPS/D-Gal-Induced Acute Liver Injury in Mice

Magnolol Prevents Acute Alcoholic Liver Damage by Activating PI3K/Nrf2/PPARγ and Inhibiting NLRP3 Signaling Pathway

Hepatocellular HO-1 mediated iNOS-induced hepatoprotection against liver ischemia reperfusion injury

Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway

Study of the selenizing Codonopsis pilosula polysaccharides protects RAW264.7 cells from hydrogen peroxide-induced injury

Lactobacillus helveticus KLDS1.8701 alleviates D-galactose-induced aging by regulating Nrf-2 and gut microbiota in mice

Platycodin D protects acetaminophen-induced hepatotoxicity by inhibiting hepatocyte MAPK pathway and apoptosis in C57BL/6J mice

Procyanidin B2 mitigates behavioral impairment and protects myelin integrity in cuprizone-induced schizophrenia in mice

α-Lipoic acid alleviates pentetrazol-induced neurological deficits and behavioral dysfunction in rats with seizures via an Nrf2 pathway

Ginsenoside Rb1 Protects the Brain from Damage Induced by Epileptic Seizure via Nrf2/ARE Signaling

Naringenin Ameliorates Behavioral Dysfunction and Neurological Deficits in a d-Galactose-Induced Aging Mouse Model Through Activation of PI3K/Akt/Nrf2 Pathway

Procyanidin B2 ameliorates carrageenan-induced chronic nonbacterial prostatitis in rats via anti-inflammatory and activation of the Nrf2 pathway

Total Flavonoid Extract from Abelmoschus manihot (L.) Medic Flowers Attenuates d-Galactose-Induced Oxidative Stress in Mouse Liver Through the Nrf2 Pathway

Apigenin exhibits protective effects in a mouse model of D-galactose-induced aging via activating the Nrf2 pathway

Antifatigue Effect of Luteolin-6-C-Neohesperidoside on Oxidative Stress Injury Induced by Forced Swimming of Rats through Modulation of Nrf2/ARE Signaling Pathways

Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway

Myricetin alleviates cuprizone-induced behavioral dysfunction and demyelination in mice by Nrf2 pathway

Hydrogen sulfide accelerates wound healing in diabetic rats

Heat shock protein 32/heme oxygenase-1 protects mouse Sertoli cells from hyperthermia-induced apoptosis by CO activation of sGC signalling pathways

Taurine attenuates oxidative stress and alleviates cardiac failure in type I diabetic rats.

Upregulation of heat shock protein 32 in Sertoli cells alleviates the impairments caused by heat shock-induced apoptosis in mouse testis

Anthocyanins from Chinese Bayberry Extract Protect β Cells from Oxidative Stress-Mediated Injury via HO-1 Upregulation

Review

Have you used Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®?

1 Review For Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®

Anti-HO-1/HMOX1 Antibody (PB9085) tracked the downregulation of HO-1/HMOX1 in ANIT-induced cholestatic rat liver via WB, with subtle non-specific bands and consistent GAPDH loading control.

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