Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®

Effects of magnolol on mice alcohol-induced liver damage in the Nrf2/HO-1 signaling pathway. After the completion of modeling and samples were collected, the liver of mice was lysed to detect the proteins by western blotting analysis. The levels of Nrf2 and HO-1 were compared with GAPDH. The data were demonstrated as means ± SD. (*P < 0.05, ***P < 0.001 and "ns" means not significant).
Index in PubMed under a CC BY license. PMID: 31920652

Trimetazidine restrained EE-induced activation of NF-κB and inactivation of HO-1/Nrf2 signaling pathways. (A–C) Western blot analysis of the protein levels of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα), P-IκBα, and nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB) in myocardial tissues. GAPDH and Histone H3 were used as loading controls. (D,E) The DNA binding activity of NF-κB was evaluated by EMSA assay. (F–G) Western blot analysis of the protein levels of heme oxygenase 1 (HO-1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2) in myocardial tissues. Each value is shown as mean ± SD ( n = 6). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, versus the indicated group.
Index in PubMed under a CC BY license. PMID: 30890937

Involvement of Nrf2/HO-1 signaling pathway in therapeutic effects of NMN. ( A ) Representative images and quantitative analysis of HO-1 protein in peri-hemorrhagic brain tissues at 24 hours after cICH. ( B ) Representative images and quantitative analysis of Nrf2 protein in peri-hemorrhagic brain tissues at 24 hours after cICH. ( C ) Representative images and quantitative analysis of Nrf2 protein distribution in cytosolic and nuclear extracts of brain peri-hemorrhagic tissues at 24 hours after cICH. * P < 0.01, n = 5 per group. NS, no significance.
Index in PubMed under a CC BY license. PMID: 28386082

Western blot analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB9085).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7- WT whole cell lysates,
Lane 2: mouse RAW264.7-HMOX1 KO whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HO-1/HMOX1 antigen affinity purified polyclonal antibody (PB9085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HO-1/HMOX1 at approximately 33 kDa. The expected band size for HO-1/HMOX1 is at 33 kDa.

Western blot analysis of HMOX1 using anti-HMOX1 antibody (PB9085).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX1 antigen affinity purified polyclonal antibody (PB9085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMOX1 at approximately 33 kDa. The expected band size for HMOX1 is at 33 kDa.

IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9085).
HMOX1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9085).
HMOX1 was detected in a paraffin-embedded section of rat alcoholic hepatitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.