Anti-Histamine H3 receptor HRH3 Antibody

Histamine H3R antibody

Boster Bio Anti-Histamine H3 receptor HRH3 Antibody catalog # PA1204. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1204
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Histamine H3 receptor HRH3 Antibody

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SKU/Catalog Number

PA1204

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Histamine H3 receptor HRH3 Antibody catalog # PA1204. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histamine H3 receptor HRH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1204)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HRH3, different from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1204 is reactive to HRH3 in Human, Mouse, Rat

Applications

PA1204 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

49 kDa

Calculated molecular weight

48.671kDa

Background of Histamine H3R

The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22% and 21.4% amino acid sequence homology with the H1 (HRH1) and H2 (HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For HRH3 (Source: Uniprot.org, NCBI)

Gene Name

HRH3

Full Name

Histamine H3 receptor

Weight

48.671kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

GPCR97; GPCR97G protein-coupled receptor 97; G-protein coupled receptor 97; H3R; HH3R; Histamine H3 R; histamine H3 receptor; Histamine H3R; histamine receptor H3; HRH3 HRH3 GPCR97, HH3R histamine receptor H3 histamine H3 receptor|G protein-coupled receptor 97|H3R

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HRH3, check out the HRH3 Infographic

HRH3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HRH3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Histamine H3 receptor HRH3 Antibody

Question

We are currently using anti-HRH3 antibody PA1204 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

The anti-HRH3 antibody (PA1204) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-30

Order DetailsPrice
PA1204

100μg

$370
PA1204-10ug

10μg sample (liquid)

$99
PA1204-Biotin

100 μg Biotin conjugated

$570
PA1204-Cy3

100 μg Cy3 conjugated

$570
PA1204-Dylight488

100 μg Dylight488 conjugated

$570
PA1204-Dylight550

100 μg Dylight550 conjugated

$570
PA1204-Dylight594

100 μg Dylight594 conjugated

$570
PA1204-FITC

100 μg FITC conjugated

$570
PA1204-HRP

100 μg HRP conjugated

$570
PA1204-APC

100 μg APC conjugated

$670
PA1204-PE

100 μg PE conjugated

$670
PA1204-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1204
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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