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COL3A1 antibody (A00788-3) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 46 Publications

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a00788 3 col3a1 primary antibodies fcm testing 8

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Flow Cytometry analysis of Hela cells using anti-Collagen III/COL3A1 antibody (A00788-3).
Overlay histogram showing Hela cells stained with A00788-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Collagen III/COL3A1 Antibody (A00788-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Anti-Collagen III/COL3A1 Antibody Picoband®

COL3A1 antibody

46 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IHC, WB

Tested Applications

COL3A1 antibody was positively detected by WB in:

mouse skin tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

COL3A1 antibody was positively detected by IHC in:

human gastric poorly differentiated adenocarcinoma tissue, human tonsil tissue, human liver cancer tissue, human lung adenocarcinoma tissue, mouse lung tissue, rat lung tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

COL3A1 antibody was positively detected by FC in:

Hela cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Mouse

Tested

Recommend

Human

Tested

Recommend

Rat

Tested

Recommend

Monkey

Predicted

Rabbit

Predicted

Bovine

Predicted

Dog

Predicted

Product Information

Product Name

Anti-Collagen III/COL3A1 Antibody Picoband®
View all COL3A1 Antibodies

Form

Lyophilized

Uniprot

P02461

Gene ID

1281

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455).

Cross-Reactivity

No cross reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

200 kDa

Calculated Molecular Weight

138.6 kDa

Background of COL3A1

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Cite This Product

Anti-Collagen III/COL3A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00788-3)

Protocols

Western Blot protocol for COL3A1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for COL3A1 antibody

Review Protocol

Flow Cytometry (FC) protocol for COL3A1 antibody

Review Protocol

Publications

Renal tubular epithelial-derived Angptl4 promotes renal fibrosis by inducing tubular cell senescence

Published: 2026-06-11

Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Application: WB

Impact Factor: 2.50

Species: Mouse

PMID: 42289177

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Free Secondary Antibody

Anti-Collagen III/COL3A1 Antibody Picoband®

COL3A1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Renal tubular epithelial-derived Angptl4 promotes renal fibrosis by inducing tubular cell senescence

Methylene blue restores NAD+/NADH homeostasis to attenuate Achilles tendinopathy via activating AIF/Mitochondrial respiratory chain complex I

Mechanisms of IL-17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

Inhibiting HSCs Activation and Ameliorating Alcoholic Liver Fibrosis by Fucoidan Targeting the TLR4/NF-κB Pathway

Novel microRNA seq-14465_x69 promotes scarless wound healing by targeting TGF-β/SMADs pathway in keratinocytes

Forskolin-loaded carboxymethyl chitosan and silk nonofiber hydrogels composite scaffolds improve skin regeneration

FAP+ gastric cancer mesenchymal stromal cells via paracrining INHBA and remodeling ECM promote tumor progression

Exosomes Derived from Antler Mesenchymal Stem Cells Promote Wound Healing by miR-21-5p/STAT3 Axis

Collagen denaturation in post-run Achilles tendons and Achilles tendinopathy: In vivo mechanophysiology and magnetic resonance imaging

Salvianolic acid B ameliorates myocardial fibrosis in diabetic cardiomyopathy by deubiquitinating Smad7

Endogenous hydrogen sulphide deficiency and exogenous hydrogen sulphide supplement regulate skin fibroblasts proliferation via necroptosis

Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway

Impaired pulp healing associated with underlying disorders in the dental pulp of rats with type 2 diabetes

Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms

Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis

Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Platelet-rich plasma pretreatment protects anterior cruciate ligament fibroblasts correlated with PI3K-Akt-mTOR pathway under hypoxia condition

Pirfenidone Attenuates Renal Tubulointerstitial Fibrosis through Inhibiting miR-21

Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial–mesenchymal transition via the TGF-β/Smad signaling pathway

The protective role of Schwann cells in bladder smooth muscle cell fibrosis

Role of NADPH oxidase pathway in renal protection induced by procyanidin B2: In L-NAME induced rat hypertension model

Effect of genistein on myocardial fibrosis in diabetic rats and its mechanism

Piperine ameliorates collagenase-induced Achilles tendon injury in the rat

Deficiency of mPGES-1 exacerbates renal fibrosis and inflammation in mice with unilateral ureteral obstruction

Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway

Protective role of ACE2-Ang-(1–7)-Mas in myocardial fibrosis by downregulating KCa3.1 channel via ERK1/2 pathway

Skin healing and collagen changes of rats after fractional erbium:yttrium aluminum garnet laser: observation by reflectance confocal microscopy with confirmed histological evidence

Hypoxia enhances the protective effects of placenta-derived mesenchymal stem cells against scar formation through hypoxia-inducible factor-1α

CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages

Mechanisms of Nerve Capping Technique in Prevention of Painful Neuroma Formation

Rapamycin Slows IgA Nephropathy Progression in the Rat

Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats

Anti-fibrotic effects of puerarin on CCl4-induced hepatic fibrosis in rats possibly through the regulation of PPAR-γ expression and inhibition of PI3K/Akt pathway

Isolation of neural crest-derived stem cells from rat embryonic mandibular processes

Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells

Thrombospondin-1 Short Hairpin RNA Suppresses Tubulointerstitial Fibrosis in the Kidney of Ureteral Obstruction by Ameliorating Peritubular Capillary Injury

The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis

Effects of exogenous urotensin II on vascular remodelling after balloon injury

Attenuation of portal hypertension by natural taurine in rats with liver cirrhosis

The role of mast cells in non-ablative laser resurfacing with 1,320 nm neodymium:yttrium–aluminium–garnet laser

Inhibition of plasminogen activator inhibitor-1 expression by siRNA in rat hepatic stellate cells

Fluvastatin decreases cardiac fibrosis possibly through regulation of TGF-β1/Smad 7 expression in the spontaneously hypertensive rats

Modified synthetic siRNA targeting tissue inhibitor of metalloproteinase-2 inhibits hepatic fibrogenesis in rats

Role of impaired peritubular capillary and hypoxia in progressive interstitial fibrosis after 56 subtotal nephrectomy of rats

Treatment of experimental hepatic fibrosis by combinational delivery of urokinase-type plasminogen activator and hepatocyte growth factor genes

Effects of pentoxifylline on the hepatic content of TGF-β1 and collagen in Schistosomiasis japonica mice with liver fibrosis

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