Anti-Collagen III/COL3A1 Antibody Picoband™

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband™ catalog # A00788-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 23 publication(s).

Product Info Summary

SKU: A00788-3
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Collagen III/COL3A1 Antibody Picoband™

See all Collagen III alpha 1/COL3A1 products

SKU/Catalog Number

A00788-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband™ catalog # A00788-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen III/COL3A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00788-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00788-3 is reactive to COL3A1 in Human, Mouse, Rat

Applications

A00788-3 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For COL3A1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02461

Gene ID

1281

Gene Name

COL3A1

Full Name

Collagen alpha-1(III) chain

Weight

138.564kDa

Superfamily

fibrillar collagen family

Alternative Names

alpha1 (III) collagen; COL3A1; Collagen 3; collagen alpha-1(III) chain; Collagen III alpha 1; collagen, fetal; collagen, type III, alpha 1; Collagen-3; EDS4A; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534 COL3A1 EDS4A, EDSVASC, PMGEDSV collagen type III alpha 1 chain collagen alpha-1(III) chain|Ehlers-Danlos syndrome type IV, autosomal dominant|alpha-1 type III collagen|alpha1 (III) collagen|collagen, fetal|collagen, type III, alpha 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on COL3A1, check out the COL3A1 Infographic

COL3A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for COL3A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00788-3 has been cited in 23 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liuyi Yang,Meng Wang,Yuan Zhou,Jing Yang,Chaoyang Ye,Chen Wang,"Shen Shuai II Recipe Attenuates Renal Interstitial Fibrosis by Improving Hypoxia via the IL-1β/c-Myc Pathway",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 5539584,13 pages,2021.https://doi.org/10.1155/2021/5539584
Species: Rat
A00788-3 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000

Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
A00788-3 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1500

Chun-Li Long,Ji-Wei Qiu,Rong-Gao Wei,et al. Effects of Yishenbupi (Tonifying-Kidney and Invigorating-Spleen) prescription on renal fibrosis. Drug Combination Therapy 2020,2(4):149-156.doi:10. 12032/DCT2020A0 28
Species: A00788-3;APP:IHC;SAMPLE:KIDNEY TISSUE;DILUTION:NA|MA1029;APP:IHC;SAMPLE:KIDNEY TISSUE;DILUTION:NA

Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
A00788-3 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:1000

Hu D,Zhang D,Liu B,Liu Y,Zhou Y,Yu Y,Shen L,Long C,Zhang D,Liu X,Lin T,He D,Xu T,Timashev P,Butnaru D,Zhang Y,Wei G.Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial-mesenchymal transition via the TGF-
Species: Human, Rat
A00788-3 usage in article: APP:IF, SAMPLE: RAT KIDNEY TISSUE,DKSS AND HUMAN UCMSC CELLS, DILUTION:1:200

Hepatoprotective effect of juglone on dimethylnitrosamine%u2011induced liver fibrosis and its effect on hepatic antioxidant defence and the expression levels of %u03B1 %u2026

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.

Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target

Effects of rhBNP on myocardial fibrosis after myocardial infarction in rats

Continuing Treatment with Salvia miltiorrhiza Injection Attenuates Myocardial Fibrosis in Chronic Iron-Overloaded Mice

Mechanisms of Nerve Capping Technique in Prevention of Painful Neuroma Formation

Effects of pentoxifylline on the hepatic content of TGF-?1 and collagen in Schistosomiasis japonica mice with liver fibrosis

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

Astragalus membranaceus?ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition?in vivo?and?in vitro

Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-?1 with concomitant induce Smad7 in human hypertrophic scar fibroblasts

Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro

Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats

Ge Ws, Wang Yj, Wu Jx, Fan Jg, Chen Yw, Zhu L. Mol Med Rep. 2014 Jun;9(6):2145-51. Doi: 10.3892/Mmr.2014.2099. Epub 2014 Apr 1. ??-Catenin Is Overexpressed In Hepatic Fibrosis And Blockage Of Wnt/??-Catenin Signaling Inhibits Hepatic Stellate Cell...

Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...

Xu Y, Zhang Ja, Xu Y, Guo Sl, Wang S, Wu D, Wang Y, Luo D, Zhou Br. Stem Cells Dev. 2015 May 1;24(9):1096-111. Doi: 10.1089/Scd.2014.0321. Epub 2015 Jan 21. Antiphotoaging Effect Of Conditioned Medium Of Dedifferentiated Adipocytes On Skin In Vivo...

Liang J, Deng X, Lin Zx, Zhao Lc, Zhang Xl. World J Gastroenterol. 2009 Sep 28;15(36):4529-37. Attenuation Of Portal Hypertension By Natural Taurine In Rats With Liver Cirrhosis.

Ge Ws, Wu Jx, Fan Jg, Wang Yj, Chen Yw. World J Gastroenterol. 2011 Sep 28;17(36):4090-8. Doi: 10.3748/Wjg.V17.I36.4090. Inhibition Of High-Mobility Group Box 1 Expression By Sirna In Rat Hepatic Stellate Cells.

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16 Customer Q&As for Anti-Collagen III/COL3A1 Antibody Picoband™

Question

I see that the anti-Collagen III/COL3A1 antibody A00788-3 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-27

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterine cervix using anti-Collagen III/COL3A1 antibody A00788-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-08

Question

We have seen staining in mouse placenta. Any tips? Is anti-Collagen III/COL3A1 antibody supposed to stain placenta positively?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

Based on literature placenta does express COL3A1. Based on Uniprot.org, COL3A1 is expressed in uterine cervix, skin fibroblast, liver, placenta, colon carcinoma, among other tissues. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886

Boster Scientific Support

Answered: 2019-12-31

Question

Does anti-Collagen III/COL3A1 antibody A00788-3 work for IHC with uterine cervix?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

According to the expression profile of uterine cervix, COL3A1 is highly expressed in uterine cervix. So, it is likely that anti-Collagen III/COL3A1 antibody A00788-3 will work for IHC with uterine cervix.

Boster Scientific Support

Answered: 2019-12-04

Question

We are currently using anti-Collagen III/COL3A1 antibody A00788-3 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

The anti-Collagen III/COL3A1 antibody (A00788-3) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-27

Question

I was wanting to use your anti-Collagen III/COL3A1 antibody for IHC for rat uterine cervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat uterine cervix identification?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

It shows on the product datasheet, A00788-3 anti-Collagen III/COL3A1 antibody has been tested for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat uterine cervix in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-22

Question

Our lab used your anti-Collagen III/COL3A1 antibody for IHC on uterine cervix a few years ago. I am using human, and I plan to use the antibody for ELISA next. We need examining uterine cervix as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

I have checked the website and datasheets of our anti-Collagen III/COL3A1 antibody and it seems that A00788-3 has been tested on human in both IHC and ELISA. Thus A00788-3 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-11

Question

Our lab want to know about to test anti-Collagen III/COL3A1 antibody A00788-3 on rat uterine cervix for research purposes, then I may be interested in using anti-Collagen III/COL3A1 antibody A00788-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

The products we sell, including anti-Collagen III/COL3A1 antibody A00788-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-07

Question

you antibody using your anti-Collagen III/COL3A1 antibody for response to radiation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

I appreciate your inquiry. This A00788-3 anti-Collagen III/COL3A1 antibody is validated on human a431 whole cell lysate, sw620 whole cell lysate, rat heart tissue, tissue lysate, skeletal muscle tissue, mouse lung. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-29

Question

Do you have a BSA free version of anti-Collagen III/COL3A1 antibody A00788-3 available?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen III/COL3A1 antibody A00788-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-17

Question

Here is the WB image, lot number and protocol we used for uterine cervix using anti-Collagen III/COL3A1 antibody A00788-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-25

Question

My question regarding product A00788-3, anti-Collagen III/COL3A1 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Wu

Verified customer

Asked: 2016-07-27

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00788-3 anti-Collagen III/COL3A1 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-07-27

Question

My team were content with the WB result of your anti-Collagen III/COL3A1 antibody . However we have observed positive staining in skin fibroblast extracellular space using this antibody. Is that expected? Could you tell me where is COL3A1 supposed to be expressed?

P. Carter

Verified customer

Asked: 2013-11-28

Answer

According to literature, skin fibroblast does express COL3A1. Generally COL3A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886

Boster Scientific Support

Answered: 2013-11-28

Question

Is a blocking peptide available for product anti-Collagen III/COL3A1 antibody (A00788-3)?

G. Wu

Verified customer

Asked: 2013-09-18

Answer

We do provide the blocking peptide for product anti-Collagen III/COL3A1 antibody (A00788-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-09-18

Question

Is this A00788-3 anti-Collagen III/COL3A1 antibody reactive to the isotypes of COL3A1?

P. Williams

Verified customer

Asked: 2013-03-14

Answer

The immunogen of A00788-3 anti-Collagen III/COL3A1 antibody is E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-03-14

Question

Would A00788-3 anti-Collagen III/COL3A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Parker

Verified customer

Asked: 2013-01-11

Answer

It shows on the product datasheet, A00788-3 anti-Collagen III/COL3A1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-01-11

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A00788-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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