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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human CXCL11/I-TAC ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CXCL11/I-TAC ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL11/I-TAC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0737)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: F22-F94
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0737 is reactive to CXCL11 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0737 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL11/I-TAC
Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.
|EK0737-CAP||Anti-Human CXCL11 Pre-coated 96-well strip microplate||1|
|EK0737-ST||Human CXCL11 Standard||2 vials, 10 ng/tube|
|EK0737-DA||Human CXCL11 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CXCL11/I-TAC PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL11/I-TAC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CXCL11/I-TAC in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CXCL11 (Source: Uniprot.Org, NCBI)
C-X-C motif chemokine 11
intercrine alpha (chemokine CxC) family
beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11 CXCL11 H174, I-TAC, IP-9, IP9, SCYB11, SCYB9B, b-R1 C-X-C motif chemokine ligand 11 C-X-C motif chemokine 11|beta-R1|chemokine (C-X-C motif) ligand 11|interferon gamma-inducible protein 9|interferon-inducible T-cell alpha chemoattractant|small inducible cytokine B11|small inducible cytokine subfamily B (Cys-X-Cys), member 11|small inducible cytokine subfamily B (Cys-X-Cys), member 9B*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CXCL11, check out the CXCL11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0737 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A correlation study between gene polymorphism of Th cell expressed chemokine receptor CXCR3 and its ligand levels with HCV infection prognosis.
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