Human CXCL11/I-TAC ELISA Kit PicoKine™

Human CXCL11/I-TAC ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0737
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human CXCL11/I-TAC ELISA Kit PicoKine™

See all CXCL11/I-TAC products

SKU/Catalog Number

EK0737

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CXCL11/I-TAC ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CXCL11/I-TAC ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0737)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-F94

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0737 is reactive to CXCL11 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0737 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0737-CAP Anti-Human CXCL11 Pre-coated 96-well strip microplate 1
EK0737-ST Human CXCL11 Standard 2 vials, 10 ng/tube
EK0737-DA Human CXCL11 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0450.1110.2550.2550.5621.0892.0192.379

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CXCL11/I-TAC ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10771216031007641565
Standard deviation7.9147.783.358.764.9482.94
CV (%)7.4%6.7%5.2%8.7%8.5%5.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL11/I-TAC in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11071221021161117.756.9%
Sample 271266766167267820.012.9%
Sample 31603150715371543154734.822.2%
*number of samples for each test n=16.

Gene/Protein Information For CXCL11 (Source: Uniprot.Org, NCBI)

Uniprot ID

O14625

Gene ID

6373

Gene Name

CXCL11

Full Name

C-X-C motif chemokine 11

Weight

10.365kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11 CXCL11 H174, I-TAC, IP-9, IP9, SCYB11, SCYB9B, b-R1 C-X-C motif chemokine ligand 11 C-X-C motif chemokine 11|beta-R1|chemokine (C-X-C motif) ligand 11|interferon gamma-inducible protein 9|interferon-inducible T-cell alpha chemoattractant|small inducible cytokine B11|small inducible cytokine subfamily B (Cys-X-Cys), member 11|small inducible cytokine subfamily B (Cys-X-Cys), member 9B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL11, check out the CXCL11 Infographic

CXCL11 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human CXCL11/I-TAC ELISA Kit PicoKine™

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

S. Gonzalez

Verified customer

Asked: 2019-07-23

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-23

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

H. Taylor

Verified customer

Asked: 2019-04-04

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-04-04

Question

Q: What is the optimal O.D. value for CXCL11 ELISA kit? I used your CXCL11 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CXCL11 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-02-18

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CXCL11 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CXCL11 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-02-18

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CXCL11 using the EK0737 Human CXCL11 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-18

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-10-18

Question

Q: can I use citrate plasma as samples in Human CXCL11 Picokine® ELISA Kit (Catalog # EK0737)?

Verified Customer

Verified customer

Asked: 2018-01-27

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CXCL11 causing disruption of its protein structure. CXCL11 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CXCL11 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-01-27

Question

Q: how to thaw whole blood sample for CXCL11 ELISA after freezing?

P. Puri

Verified customer

Asked: 2017-07-30

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL11 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2017-07-30

Question

Q: how do I analyze ELISA data? I have performed CXCL11 level in plasma.

L. Anand

Verified customer

Asked: 2017-03-16

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-03-16

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