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- Table of Contents
MYBL1 antibody (A05803-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of U87 cells using anti-v-Myb/MYBL1 antibody (A05803-2).
Overlay histogram showing U87 cells stained with A05803-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-v-Myb/MYBL1 Antibody (A05803-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U87 cells stained with A05803-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-v-Myb/MYBL1 Antibody (A05803-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-v-Myb/MYBL1 Antibody Picoband®
MYBL1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
MYBL1 antibody was positively detected by WB in:
human T-47D whole cell, human A431 whole cell, human PC-3 whole cell, human U-87MG whole cell, human A549 whole cell, rat stomach tissue, mouse stomach tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MYBL1 antibody was positively detected by FC in:
U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-v-Myb/MYBL1 Antibody Picoband®
View all MYBL1 Antibodies
View all MYBL1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human v-Myb/MYBL1 recombinant protein (Position: K199-L752).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
86 kDa
Calculated Molecular Weight
85.9 kDa
Background of MYBL1
MYB proto-oncogene like 1 is a protein that in humans is encoded by the MYBL1 gene. A-Myb is expressed at specific times in reproductive tissues, some neural cells, and a subset of normal and neoplastic B lymphocytes. Both A-Myb and B-Myb are expressed in t (14:18) lymphoma cells where they then inhibit cell arrest and apoptotic signaling. Expression of B-Myb rescues cells from p53-induced G1 phase arrest that is mediated by p21, while A-Myb functions as an anti-apoptotic factor by effectively activating the bcl-2 promoter and thereby up-regulating Bcl-2 expression.
Cite This Product
Anti-v-Myb/MYBL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05803-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
92%
High Homology
Cow
91%
High Homology
Sheep
91%
High Homology
Mouse
90%
High Homology
Rat
90%
High Homology
Dog
82%
Moderate Homology
Zebrafish
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MYBL1 antibody
Flow Cytometry (FC) protocol for MYBL1 antibody
Publications
Application:
Species:
Histone methyltransferase G9a drives vascular smooth muscle cell proliferation and intimal hyperplasia in mice
Published: 2026-02-09
Journal: ACTA PHARMACOLOGICA SINICA
Application: WB
Impact Factor: 8.40
Species: Rat
PMID: 41663737
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Product Promise
We promise Anti-v-Myb/MYBL1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05803-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026