Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)

Collagen III alpha 1/COL3A1 antibody

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9) catalog # M00788. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: M00788
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

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Product Name

Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)

View all Collagen III alpha 1/COL3A1 Antibodies

SKU/Catalog Number

M00788

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9) catalog # M00788. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00788)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

9H9

Isotype

Mouse IgG2b

Immunogen

E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222- E1455).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00788 is reactive to COL3A1 in Human, Mouse, Rat

Applications

M00788 is guaranteed for IHC, WB Boster Guarantee

Background of Collagen III alpha 1/COL3A1

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For COL3A1 (Source: Uniprot.org, NCBI)

Uniprot ID

P02461

Gene ID

1281

Gene Name

COL3A1

Full Name

Collagen alpha-1(III) chain

Weight

138.564kDa

Superfamily

fibrillar collagen family

Alternative Names

alpha1 (III) collagen; COL3A1; Collagen 3; collagen alpha-1(III) chain; Collagen III alpha 1; collagen, fetal; collagen, type III, alpha 1; Collagen-3; EDS4A; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534 COL3A1 EDS4A, EDSVASC, PMGEDSV collagen type III alpha 1 chain collagen alpha-1(III) chain|Ehlers-Danlos syndrome type IV, autosomal dominant|alpha-1 type III collagen|alpha1 (III) collagen|collagen, fetal|collagen, type III, alpha 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COL3A1, check out the COL3A1 Infographic

COL3A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

M00788 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues

ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes

Liuyi Yang,Meng Wang,Yuan Zhou,Jing Yang,Chaoyang Ye,Chen Wang,"Shen Shuai II Recipe Attenuates Renal Interstitial Fibrosis by Improving Hypoxia via the IL-1β/c-Myc Pathway",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 5539584,13 pages,2021.https://doi.org/10.1155/2021/5539584
Species: Rat

Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
M00788 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1500

Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
M00788 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:1000

Hepatoprotective effect of juglone on dimethylnitrosamine%u2011induced liver fibrosis and its effect on hepatic antioxidant defence and the expression levels of %u03B1 %u2026

Ge Ws, Wu Jx, Fan Jg, Wang Yj, Chen Yw. World J Gastroenterol. 2011 Sep 28;17(36):4090-8. Doi: 10.3748/Wjg.V17.I36.4090. Inhibition Of High-Mobility Group Box 1 Expression By Sirna In Rat Hepatic Stellate Cells.

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15 Customer Q&As for Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)

Question

We are currently using anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

The anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) (M00788) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-06

Question

Can you help my question with product M00788, anti-Collagen III/COL3A1 antibody (monoclonal, 9H9). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00788 anti-Collagen III/COL3A1 antibody (monoclonal, 9H9), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-27

Question

Do you have a BSA free version of anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 available?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-23

Question

I see that the anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 works with IHC-P, what is the protocol used to produce the result images on the product page?

Z. Jackson

Verified customer

Asked: 2019-11-06

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-06

Question

Will anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 work for IHC-P with uterine cervix?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

According to the expression profile of uterine cervix, COL3A1 is highly expressed in uterine cervix. So, it is likely that anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 will work for IHC-P with uterine cervix.

Boster Scientific Support

Answered: 2019-06-27

Question

We bought anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) for WB on colon carcinoma in the past. I am using rat, and We want to use the antibody for IHC-P next. I would like examining colon carcinoma as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

O. Collins

Verified customer

Asked: 2019-01-23

Answer

I viewed the website and datasheets of our anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) and I see that M00788 has been tested on rat in both WB and IHC-P. Thus M00788 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-01-23

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterine cervix using anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-04-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-06

Question

We have been able to see staining in rat skin fibroblast. Do you have any suggestions? Is anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) supposed to stain skin fibroblast positively?

Verified Customer

Verified customer

Asked: 2018-03-01

Answer

Based on literature skin fibroblast does express COL3A1. Based on Uniprot.org, COL3A1 is expressed in uterine cervix, skin fibroblast, liver, placenta, colon carcinoma, among other tissues. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886

Boster Scientific Support

Answered: 2018-03-01

Question

Does M00788 anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-02-20

Answer

As indicated on the product datasheet, M00788 anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-20

Question

I have attached the WB image, lot number and protocol we used for uterine cervix using anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-12-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-20

Question

I was wanting to use your anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) for IHC-P for human uterine cervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterine cervix identification?

V. Li

Verified customer

Asked: 2017-10-06

Answer

It shows on the product datasheet, M00788 anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) has been validated for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterine cervix in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-06

Question

We need to test anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 on human uterine cervix for research purposes, then I may be interested in using anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Johnson

Verified customer

Asked: 2016-09-07

Answer

The products we sell, including anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-09-07

Question

Is this M00788 anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) reactive to the isotypes of COL3A1?

N. Lewis

Verified customer

Asked: 2015-06-01

Answer

The immunogen of M00788 anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) is E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222- E1455). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-06-01

Question

We were happy with the WB result of your anti-Collagen III/COL3A1 antibody (monoclonal, 9H9). However we have seen positive staining in skin fibroblast extracellular space using this antibody. Is that expected? Could you tell me where is COL3A1 supposed to be expressed?

N. Li

Verified customer

Asked: 2014-03-06

Answer

Based on literature, skin fibroblast does express COL3A1. Generally COL3A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886

Boster Scientific Support

Answered: 2014-03-06

Question

Is a blocking peptide available for product anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) (M00788)?

N. Walker

Verified customer

Asked: 2013-07-23

Answer

We do provide the blocking peptide for product anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) (M00788). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-07-23

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Total: $315

SKU:M00788

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M00788
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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