Anti-Hu CD103 Purified ITGAE Monoclonal Antibody

Product Name

Anti-Hu CD103 Purified ITGAE Monoclonal Antibody

View all ITGAE Antibodies

SKU/Catalog Number

M07091-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD103 Purified ITGAE Monoclonal Antibody (Catalog# M07091-1). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human, Primate.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD103 Purified ITGAE Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07091-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

Ber-ACT8

Isotype

Mouse IgG1 kappa

Immunogen

HTLV-1 induced human T cell line MAPS16. The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M07091-1 is reactive to ITGAE in Human, Primate

Observed Molecular Weight

42 kDa

Calculated molecular weight

130.2 kDa

Background of ITGAE

CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M07091-1 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

See full target information ITGAE

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