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- Table of Contents
Itgae antibody (A07091-2) performs well in ELISA, IHC and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (A07091-2).
CD103/Itgae was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD103/Itgae Antibody (A07091-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CD103/Itgae was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD103/Itgae Antibody (A07091-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-CD103/Itgae Antibody
Itgae antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC |
Tested Applications
Itgae antibody was positively detected by IHC in:
mouse colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
ELISA
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-CD103/Itgae Antibody
View all ITGAE Antibodies
View all ITGAE Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD103/Itgae recombinant protein (Position: F20-E1061).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
128.9 kDa
Background of Itgae
ITGAE(INTEGRIN, ALPHA-E), also known as CD103, is an integrin protein that in human is encoded by the ITGAE gene. The ITGAE gene is mapped to chromosome 17p13 by inclusion within a BAC contig. ITGAE is expressed widely on intraepithelial lymphocyte(IEL) T cells(both alphabeta T cells and gammadelta T cells) and on some peripheral regulatory T cells(Tregs) which is important for decreasing the immune response and appears to play a crucial role in the prevention of autoimmune diseases. ITGAE shares overall homology with other alpha integrins but has a unique 55-amino acid "extra" region located just N-terminal to the I domain that contains 18 consecutive charged residues and a proteolytic cleavage site.
Cite This Product
Anti-CD103/Itgae Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07091-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
90%
High Homology
Human
70%
Moderate Homology
Cow
70%
Moderate Homology
Sheep
70%
Moderate Homology
Monkey
70%
Moderate Homology
Dog
69%
Low homology
Rabbit
67%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Itgae antibody
Publications
Application:
Species:
Immunoregulatory mechanisms underlying vitexin-mediated attenuation of concanavalin A-induced liver injury
Published: 2026-04-21
Journal: PHYTOMEDICINE
Application: mIF
Impact Factor: 8.30
Species: Mouse
PMID: 42054738
Role of dendritic cell-mediated abnormal immune response in visceral hypersensitivity
Published: 2015-08-15
Journal: International Journal of Clinical and Experimental Medicine
Application: IHC
Impact Factor: 1.28
Species: Rat
PMID: 26550249
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Product Promise
We promise Anti-CD103/Itgae Antibody performs excellently in relevant applications for ELISA, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A07091-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026