Mouse Mer ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MER/MERTK. 96wells/kit, with removable strips.
Mouse Mer ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates and serum.|
|Sample Volume:||100μl per well|
|Product Name||Mouse Mer ELISA Kit PicoKine™
See all Mer primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Mer/MERTK with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mer/MERTK tissue specificity: Expressed predominantly in the hematopoietic lineages: macrophages, NK cells, NKT cells, dendritic cells and platelets. .|
|Cite This Product||Mouse Mer ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0811)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates and serum.|
|Immunogen/Standard||Expression system for standard: sf21; Immunogen sequence: E23-F498|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Mertk (Source: Uniprot.org, NCBI)
|NCBI Gene Id||17289|
|Species Of This Entry||Mouse|
|Protein Name||Tyrosine-protein kinase Mer|
|Superfamily||protein kinase superfamily|
|Alternative Names||Mer|c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 18.104.22.168; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Gamma-carboxylation; Oxidation; Dephosphorylation|
|Gene Location||2|2 F1, on Chromasome 2, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Mertk (Source: Uniprot.org, NCBI)
|Protein Function||Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.|
|Tissue Specificity||Expressed predominantly in the hematopoietic lineages: macrophages, NK cells, NKT cells, dendritic cells and platelets.|
|Related Diseases||Retinitis Pigmentosa; Retinal Degeneration; Myeloproliferative Syndrome, Transient; Inflammation; Dystrophy; Malignant Neoplasms; Tissue Adhesions; Retinal Dystrophies; Autoimmunity; Autoimmune Reaction; Neoplasms; Blind Vision; Autoimmune Diseases; Cell Transformation, Neoplastic; Atherosclerosis; Malignant Paraganglionic Neoplasm; Lupus Erythematosus, Systemic; Retinal Diseases|
|Related Pathways||Phagocytosis; Cell Adhesion; Apoptotic Cell Clearance; Secretion; Pathogenesis; Immune Response; Platelet Aggregation; Cell Proliferation; Cytokine Production; Cell Activation; Cell Migration; Coagulation; Cell Death; Oncogenesis; Inflammatory Response; Innate Immune Response; Localization; Cell Growth; Angiogenesis; Myelination|
|Background||Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium(RPE) as a regulator of rod outer segments fragments phagocytosis. This gene also plays an important role in inhibition of Toll-like receptors(TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.|
|Scientific References||PMID: 7784083 by Graham D.K., et al. Cloning and developmental expression analysis of the murine c-mer tyrosine kinase.|
PMID: 10227296 by Lu Q., et al. Tyro-3 family receptors are essential regulators of mammalian spermatogenesis.
Kit Components And Assay QC Data Details Of Mouse Mer ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0811-CAP||96-well plate precoated with anti-Mouse Mer antibody||1|
|EK0811-ST||lyophilized recombinant Mouse Mer standard||10ng/tube|
|EK0811-DA||biotinylated anti-Mouse Mer antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0811 Mouse Mer ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse Mer ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse Mer ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (EK0811)
Domestically in USA and Canada, ships next business day if available in stock.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.