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Product Info Summary
|Reactive Species:||Human, Rat|
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Anti-ID2 Antibody Picoband™
Boster Bio Anti-ID2 Antibody Picoband™ catalog # A00417-1. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ID2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00417-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human ID2 (86-120aa VSLHHQRPGQNQASRTPLTTLNTDISILSLQASEF), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00417-1 is reactive to ID2 in Human, Rat
A00417-1 is guaranteed for WB Boster Guarantee
Background of ID2
DNA-binding protein inhibitor ID-2 is a protein that in humans is encoded by the ID2 gene. The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ID2 using anti-ID2 antibody (A00417-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ID2 antigen affinity purified polyclonal antibody (Catalog # A00417-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ID2 at approximately 15KD. The expected band size for ID2 is at 15KD.
Gene/Protein Information For ID2 (Source: Uniprot.org, NCBI)
DNA-binding protein inhibitor ID-2
BHLHB26; bHLHb26cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID2; DNA-binding protein inhibitor ID-2; GIG8; helix-loop-helix protein ID2; ID2; ID2A; ID2H; inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; MGC26389 ID2 GIG8A, ID2H, bHLHb26, ID2 inhibitor of DNA binding 2 DNA-binding protein inhibitor ID-2|DNA-binding protein inhibitor ID2|cell growth-inhibiting gene 8|class B basic helix-loop-helix protein 26|helix-loop-helix protein ID2|inhibitor of DNA binding 2, HLH protein|inhibitor of DNA binding 2, dominant negative helix-loop-helix protein|inhibitor of differentiation 2*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on ID2, check out the ID2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ID2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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