Anti-Tyrosine-protein kinase Mer MERTK Antibody

Boster Bio Anti-Tyrosine-protein kinase Mer MERTK Antibody catalog # PA2276. Tested in WB applications. This antibody reacts with Human, Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: PA2276
Size: 100μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Tyrosine-protein kinase Mer MERTK Antibody

See all Mer products

SKU/Catalog Number

PA2276

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Tyrosine-protein kinase Mer MERTK Antibody catalog # PA2276. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Tyrosine-protein kinase Mer MERTK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2276)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MERTK (132-148aa KDGKELLGAHHAITQFY), different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2276 is reactive to MERTK in Human, Mouse

Applications

PA2276 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For MERTK (Source: Uniprot.Org, NCBI)

Uniprot ID

Q12866

Gene ID

10461

Gene Name

MERTK

Full Name

Tyrosine-protein kinase Mer

Weight

110.249kDa

Superfamily

protein kinase superfamily

Alternative Names

c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer MERTK MER, RP38, Tyro12, c-Eyk, c-mer MER proto-oncogene, tyrosine kinase tyrosine-protein kinase Mer|MER receptor tyrosine kinase|STK kinase|c-mer proto-oncogene tyrosine kinase|proto-oncogene c-Mer|receptor tyrosine kinase MerTK

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MERTK, check out the MERTK Infographic

MERTK infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MERTK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA2276 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Functional screen identifies kinases driving prostate cancer visceral and bone metastasis

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16 Customer Q&As for Anti-Tyrosine-protein kinase Mer MERTK Antibody

Question

We have observed staining in human right adrenal gland cortex. Are there any suggestions? Is anti-MERTK antibody supposed to stain right adrenal gland cortex positively?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

From what I have seen in literature right adrenal gland cortex does express MERTK. From what I have seen in Uniprot.org, MERTK is expressed in right adrenal gland cortex, peripheral blood leukocyte, plasma, among other tissues. Regarding which tissues have MERTK expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 8086340, 11062461
Plasma, Pubmed ID: 16335952

Boster Scientific Support

Answered: 2020-04-07

Question

Would anti-MERTK antibody PA2276 work on primate WB with plasma?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

Our lab technicians have not validated anti-MERTK antibody PA2276 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MERTK antibody PA2276 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate plasma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-19

Question

Can you help my question with product PA2276, anti-MERTK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2276 anti-MERTK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-03

Question

Is this PA2276 anti-MERTK antibody reactive to the isotypes of MERTK?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

The immunogen of PA2276 anti-MERTK antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human MERTK(132-148aa KDGKELLGAHHAITQFY), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-17

Question

Does PA2276 anti-MERTK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

As indicated on the product datasheet, PA2276 anti-MERTK antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-11

Question

I was wanting to use your anti-MERTK antibody for WB for human plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human plasma identification?

Verified Customer

Verified customer

Asked: 2019-04-11

Answer

As indicated on the product datasheet, PA2276 anti-MERTK antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-11

Question

My question regards to test anti-MERTK antibody PA2276 on human plasma for research purposes, then I may be interested in using anti-MERTK antibody PA2276 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

The products we sell, including anti-MERTK antibody PA2276, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-30

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-MERTK antibody PA2276. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-08-10

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-10

Question

Our lab were happy with the WB result of your anti-MERTK antibody. However we have been able to see positive staining in right adrenal gland cortex membrane using this antibody. Is that expected? Could you tell me where is MERTK supposed to be expressed?

L. Johnson

Verified customer

Asked: 2018-07-17

Answer

From what I have seen in literature, right adrenal gland cortex does express MERTK. Generally MERTK expresses in membrane. Regarding which tissues have MERTK expression, here are a few articles citing expression in various tissues:
Peripheral blood leukocyte, Pubmed ID: 8086340, 11062461
Plasma, Pubmed ID: 16335952

Boster Scientific Support

Answered: 2018-07-17

Question

Does anti-MERTK antibody PA2276 work for WB with plasma?

Verified Customer

Verified customer

Asked: 2018-04-20

Answer

According to the expression profile of plasma, MERTK is highly expressed in plasma. So, it is likely that anti-MERTK antibody PA2276 will work for WB with plasma.

Boster Scientific Support

Answered: 2018-04-20

Question

I have attached the WB image, lot number and protocol we used for plasma using anti-MERTK antibody PA2276. Please let me know if you require anything else.

G. Anderson

Verified customer

Asked: 2018-02-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-19

Question

We are currently using anti-MERTK antibody PA2276 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?

D. Collins

Verified customer

Asked: 2017-07-27

Answer

The anti-MERTK antibody (PA2276) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-27

Question

I see that the anti-MERTK antibody PA2276 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-05-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-05-26

Question

Is a blocking peptide available for product anti-MERTK antibody (PA2276)?

E. Walker

Verified customer

Asked: 2016-09-15

Answer

We do provide the blocking peptide for product anti-MERTK antibody (PA2276). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-09-15

Question

My lab would like using your anti-MERTK antibody for phagocytosis studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

R. Krishna

Verified customer

Asked: 2015-10-08

Answer

I appreciate your inquiry. This PA2276 anti-MERTK antibody is validated on hela whole cell lysate, a549 whole cell lysate, nih whole cell lysate, 22rv1 whole cell lysate. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-10-08

Question

Do you have a BSA free version of anti-MERTK antibody PA2276 available?

J. Jackson

Verified customer

Asked: 2013-10-11

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MERTK antibody PA2276 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-10-11

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA2276
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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