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- Table of Contents
Il1b antibody (M00101-3) performs well in Flow Cytometry, IP, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Western blot analysis of IL1 beta expression in RAW 264.7 cell treated with Lipopolysaccharide (LPS) cell lysate.
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Anti-IL-1 beta Rabbit Monoclonal Antibody
Il1b antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | Flow Cytometry, IP, WB |
Tested Applications
Il1b antibody was positively detected by WB in:
RAW 264
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL-1 beta Rabbit Monoclonal Antibody
View all IL1B Antibodies
View all IL1B Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
28I10
Isotype
IgG
Immunogen
A synthesized peptide derived from human IL-1 beta
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
30.9 kDa
Cite This Product
Anti-IL-1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00101-3)
Protocols
Western Blot protocol for Il1b antibody
Publications
Application:
Species:
C-Myc Inhibition Using 10058-F4 Suppressed Ischemia Reperfusion Induced Cardiac Microvascular Endothelial Cell Pyroptosis through the Sam68/NF-κB Pathway
Published: 2026-07-31
Journal: International Heart Journal
Application: WB
Impact Factor: 1.40
Species: Rat
PMID: 42543667
Molecular mechanisms of dragon’s blood in treating ulcerative colitis based on NF-κb/NLPR3/Caspase-1 pyroptosis signaling pathway
Published: 2025-09-19
Journal: PLoS One
Application: WB
Impact Factor: 2.60
Species: Mouse
PMID: 40971395
Investigating the molecular mechanism of epimedium herb in treating rheumatoid arthritis through network pharmacology, molecular docking, and experimental validation
Published: 2025-01-16
Journal: MOLECULAR DIVERSITY
Application: IHC
Impact Factor: 3.80
Species: Mouse
PMID: 39821498
LMWH prevents thrombo-inflammation in the placenta via HBEGF-AKT signalling
Published: 2024-06-28
Journal: Blood Advances
Application: WB
Impact Factor: 7.40
Species: Mouse
PMID: 38941535
Microglial SLC25A28 Deficiency Ameliorates the Brain Injury After Intracerebral Hemorrhage in Mice by Restricting Aerobic Glycolysis
Published: 2023-12-12
Journal: INFLAMMATION
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 38085466
Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1β/miR-101/Lin28B Signaling Pathway
Published: 2016-08-24
Journal: INTEGRATIVE CANCER THERAPIES
Application: WB,IHC
Impact Factor: 1.71
Species: Mouse
PMID: 27562754
The natural compound celastrol inhibits necroptosis and alleviates ulcerative colitis in mice
Published: 2015-10-09
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 2.47
Species: Mouse
PMID: 26454701
NLRP3 Inflammasome Sequential Changes in Staphylococcus aureus-Induced Mouse Model of Acute Rhinosinusitis
Published: 2014-09-09
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 2.34
Species: Mouse
PMID: 25207596
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Product Promise
We promise Anti-IL-1 beta Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00101-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026