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Product Info Summary
| SKU: | M00101-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-IL-1 beta Rabbit Monoclonal Antibody
SKU/Catalog Number
M00101-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-IL-1 beta Rabbit Monoclonal Antibody catalog # M00101-3. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00101-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
28I10
Isotype
IgG
Immunogen
A synthesized peptide derived from human IL-1 beta
Reactive Species
M00101-3 is reactive to Il1b in Mouse, Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
30.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00101-3 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
FC 1:50
Positive Control
WB: RAW 264
Validation Images & Assay Conditions
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Western blot analysis of IL1 beta expression in RAW 264.7 cell treated with Lipopolysaccharide (LPS) cell lysate.
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Western blot analysis of IL1B using anti-IL1B antibody (M00101-3).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: recombinant rat IL1B protein.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified monoclonal antibody (M00101-3) at 1:500 overnight at 4°C, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL1B at approximately 17 kDa. The expected band size for IL1B is at 17 kDa.
Specific Publications For Anti-IL-1 beta Rabbit Monoclonal Antibody (M00101-3)
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