Anti-IL1 beta Antibody

Product Name

Anti-IL1 beta Antibody

View all IL1B Antibodies

SKU/Catalog Number

A00101-3

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-IL1 beta Antibody (Catalog # A00101-3). Tested in IF, IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store Anti-IL-1 beta antibody at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-IL1 beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00101-3)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

This antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli.  The MW of recombinant mouse IL-1ß was 17 kDa.

Reactive Species

A00101-3 is reactive to IL1B in Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

30.9 kDa

Background of IL1B

IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Anti-IL-1 beta antibody is ideal for investigators involved in Cardiovascular and Immunology research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00101-3 is guaranteed for ELISA, Flow Cytometry, IP, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:5,000
Flow Cytometry: User optimized
IHC: 1:50-1:250
IF Microscopy: 1:50-1:250
IP: 1:200-1:800
WB: 1:500 - 1:2,000
Neutralization: User optimized
Anti-Mouse IL-1ß has been tested for use in immunohistochemistry, immunoblotting and immunofluorescence. This antibody is useful in ELISA, neutralizations, radioimmunoassays, flow cytometry, and immunoprecipitation. It recognizes the 17,000 MW mature IL-1ß. For immunoblots, typically, IL-1ß is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1ß. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1ß activity in bioassays. It does not neutralize the biological activity IL-1α. It does not neutralize the biological activity of human or primate IL-1ß. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Validation Images & Assay Conditions

See full target information IL1B

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