Product Info Summary
SKU: | PB9606 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Rat |
Host: | Rabbit |
Application: | ELISA |
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Product info
Product Name
Anti-IL1 beta/IL1B Antibody Picoband™
View all IL-1 beta/IL-1F2 Antibodies
SKU/Catalog Number
PB9606
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL1 beta/IL1B Antibody Picoband™ catalog # PB9606. Tested in ELISA applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL1 beta/IL1B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9606)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat IL-1 beta, different from the related human sequence by five amino acids, and from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9606 is reactive to IL1B in Rat
Applications
PB9606 is guaranteed for ELISA Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
30.748kDa
Background of IL-1 beta/IL-1F2
Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
30.748kDa
Superfamily
IL-1 family
Alternative Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL1 beta/IL1B Antibody Picoband™ (PB9606)
Hello CJ!
PB9606 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Exogenous %u03B1-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats
In vivo anti-inflammatory activity of caffeoylquinic acid derivatives from Solidago virgaurea in rats
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-IL1 beta/IL1B Antibody Picoband™
Question
Can you help my question with product PB9606, anti-IL1 beta/IL1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9606 anti-IL1 beta/IL1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-28
Question
Do you have a BSA free version of anti-IL1 beta/IL1B antibody PB9606 available?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL1 beta/IL1B antibody PB9606 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-11
Question
Here is the WB image, lot number and protocol we used for macrophage using anti-IL1 beta/IL1B antibody PB9606. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for macrophage using anti-IL1 beta/IL1B antibody PB9606. Let me know if you need anything else.
G. Wu
Verified customer
Asked: 2019-11-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use your anti-IL1 beta/IL1B antibody for ELISA for rat macrophage on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat macrophage identification?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
As indicated on the product datasheet, PB9606 anti-IL1 beta/IL1B antibody has been tested for ELISA on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat macrophage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-31
Question
Does anti-IL1 beta/IL1B antibody PB9606 work for ELISA with macrophage?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
According to the expression profile of macrophage, IL1B is highly expressed in macrophage. So, it is likely that anti-IL1 beta/IL1B antibody PB9606 will work for ELISA with macrophage.
Boster Scientific Support
Answered: 2019-04-02
Question
We need to test anti-IL1 beta/IL1B antibody PB9606 on rat macrophage for research purposes, then I may be interested in using anti-IL1 beta/IL1B antibody PB9606 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The products we sell, including anti-IL1 beta/IL1B antibody PB9606, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-08
Question
Is this PB9606 anti-IL1 beta/IL1B antibody reactive to the isotypes of IL1B?
V. Mitchell
Verified customer
Asked: 2019-01-01
Answer
The immunogen of PB9606 anti-IL1 beta/IL1B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat IL-1 beta (202-229aa DPKQYPKKKMEKRFVFNKIEVKTKVEFE), different from the related human sequence by five amino acids, and from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-01
Question
Our team were well pleased with the WB result of your anti-IL1 beta/IL1B antibody. However we have been able to see positive staining in monocyte cytoplasm using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
From what I have seen in literature, monocyte does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2018-10-30
Question
We have been able to see staining in rat skin. Any tips? Is anti-IL1 beta/IL1B antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
Based on literature skin does express IL1B. Based on Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2017-11-14
Question
Will PB9606 anti-IL1 beta/IL1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Mangal
Verified customer
Asked: 2016-08-11
Answer
As indicated on the product datasheet, PB9606 anti-IL1 beta/IL1B antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-11
Question
Is a blocking peptide available for product anti-IL1 beta/IL1B antibody (PB9606)?
K. Rodriguez
Verified customer
Asked: 2016-06-30
Answer
We do provide the blocking peptide for product anti-IL1 beta/IL1B antibody (PB9606). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-06-30
Question
I see that the anti-IL1 beta/IL1B antibody PB9606 works with ELISA, what is the protocol used to produce the result images on the product page?
A. Johnson
Verified customer
Asked: 2016-01-13
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-13
Question
We are currently using anti-IL1 beta/IL1B antibody PB9606 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on bovine tissues as well?
K. Banerjee
Verified customer
Asked: 2014-11-24
Answer
The anti-IL1 beta/IL1B antibody (PB9606) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-24