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Product Info Summary
| SKU: | M00368 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-iNOS NOS2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00368
BM4828 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-iNOS NOS2 Rabbit Monoclonal Antibody catalog # M00368. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-iNOS NOS2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00368)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IED-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human iNOS
Reactive Species
M00368 is reactive to NOS2 in Human, Mouse
Observed Molecular Weight
131 kDa
Calculated molecular weight
131.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00368 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of iNOS expression in Human fetal brain lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Raw264.7 cells treated with LPS, using iNOS Antibody.
Specific Publications For Anti-iNOS NOS2 Rabbit Monoclonal Antibody (M00368)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-iNOS NOS2 Rabbit Monoclonal Antibody
Question
Can M00368 antibody be used in IHC?
Verified customer
Asked: 2019-11-13
Answer
No, the Anti-INOS NOS2 Rabbit Monoclonal Antibody (M00368) cannot be used for Immunohistochemistry experiment.
Boster Scientific Support
Answered: 2019-11-13
Question
We are currently using anti-iNOS Rabbit Monoclonal antibody M00368 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The anti-iNOS Rabbit Monoclonal antibody (M00368) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-15
Question
Our team were satisfied with the WB result of your anti-iNOS Rabbit Monoclonal antibody. However we have been able to see positive staining in chondrocyte cytoplasm using this antibody. Is that expected? Could you tell me where is NOS2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
According to literature, chondrocyte does express NOS2. Generally NOS2 expresses in cytoplasm, cytosol. Regarding which tissues have NOS2 expression, here are a few articles citing expression in various tissues:
Airway epithelium, Pubmed ID: 7544004
Articular chondrocyte, Pubmed ID: 7522054
Cardiac myocyte, Pubmed ID: 9160867
Chondrocyte, Pubmed ID: 7504305
Colon adenocarcinoma, Pubmed ID: 7692964
Glioblastoma, Pubmed ID: 7528267, 7531687
Kidney, Pubmed ID: 7532248
Liver, Pubmed ID: 7682706
Retina, Pubmed ID: 7528017
Boster Scientific Support
Answered: 2019-07-25
Question
We have observed staining in mouse airway epithelium. What should we do? Is anti-iNOS Rabbit Monoclonal antibody supposed to stain airway epithelium positively?
S. Jha
Verified customer
Asked: 2017-02-07
Answer
Based on literature airway epithelium does express NOS2. Based on Uniprot.org, NOS2 is expressed in cartilage tissue, colon adenocarcinoma, liver, chondrocyte, articular chondrocyte, retina, glioblastoma, airway epithelium, cardiac myocyte, kidney, among other tissues. Regarding which tissues have NOS2 expression, here are a few articles citing expression in various tissues:
Airway epithelium, Pubmed ID: 7544004
Articular chondrocyte, Pubmed ID: 7522054
Cardiac myocyte, Pubmed ID: 9160867
Chondrocyte, Pubmed ID: 7504305
Colon adenocarcinoma, Pubmed ID: 7692964
Glioblastoma, Pubmed ID: 7528267, 7531687
Kidney, Pubmed ID: 7532248
Liver, Pubmed ID: 7682706
Retina, Pubmed ID: 7528017
Boster Scientific Support
Answered: 2017-02-07
Question
We bought anti-iNOS Rabbit Monoclonal antibody for IP on colon adenocarcinoma in the past. I am using mouse, and We want to use the antibody for WB next. I was wanting to use examining colon adenocarcinoma as well as chondrocyte in our next experiment. Could give a recommendation on which antibody would work the best for WB?
J. Edwards
Verified customer
Asked: 2014-04-09
Answer
I looked at the website and datasheets of our anti-iNOS Rabbit Monoclonal antibody and it seems that M00368 has been validated on mouse in both IP and WB. Thus M00368 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-04-09
