Anti-Kallistatin/SERPINA4 Antibody

Rabbit IgG polyclonal antibody for Kallistatin(SERPINA4) detection. Tested with WB, IHC-P in Human.

SKU PA1638
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB


Product Name Anti-Kallistatin/SERPINA4 Antibody
See all SERPINA4 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1638
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Polyclonal antibody for Kallistatin/SERPINA4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Kallistatin/SERPINA4 information: Molecular Weight: 48542 MW; Subcellular Localization: Secreted; Tissue Specificity: Expressed by the liver and secreted in plasma.
Cite This Product Anti-Kallistatin/SERPINA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1638)
Specificity Anti-Kallistatin/SERPINA4 Antibody (PA1638) reacts with Human SERPINA4, in native form and recombinant. Superfamily members of SERPINA4 are not reactive to PA1638.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Kallistatin(384-401aa AIKFFSAQTNRHILRFNR).
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-Kallistatin/SERPINA4 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Kallistatin/SERPINA4 Antibody (PA1638).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions


Anti-Kallistatin antibody, PA1638, IHC(P)
IHC(P): Human Liver Cancer Tissue


Anti-Kallistatin antibody, PA1638, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: JURKAT Cell Lysate


Anti-Kallistatin antibody, PA1638, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SKOV Cell Lysate

Target Info

Protein Target Info (Source:

Uniprot Id P29622
Gene Name SERPINA4
Protein Name Kallistatin
Tissue Specificity Expressed by the liver and secreted in plasma.
Alternative Names Kallistatin;Kallikrein inhibitor;Peptidase inhibitor 4;PI-4;Serpin A4;SERPINA4;KST, PI4;
Subcellular Localization Secreted.
Molecular Weight 48542 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.


Protein Function Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. .
Research Areas Human

*You can search these to find other products in these research areas.
Background Serpina 4, also called Kallistatin, PI4 or TISSUE KALLIKREIN INHIBITOR, is a protein that in humans is encoded by the SERPINA4 gene. The serpina4 is a member of the serpin superfamily and represents a major inhibitor of human tissue kallikrein in the circulation. The SERPINA4 gene is localized to 14q32.13. The translated amino acid sequence of serpina4 matches the protein sequence and shares 44 to 46% sequence identity with other serpin family members. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. And serpina4 also plays a role in neointima hyperplasia.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)



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Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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