Product Info Summary
SKU: | M00683 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IRE alpha ERN1 Monoclonal Antibody
View all IRE1 alpha Antibodies
SKU/Catalog Number
M00683
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IRE alpha ERN1 Monoclonal Antibody catalog # M00683. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRE alpha ERN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00683)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
9F2
Isotype
IgG
Immunogen
Purified recombinant fragment of human IRE1alpha (aa282-433) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00683 is reactive to ERN1 in Human
Applications
M00683 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
109.735kDa
Background of IRE1 alpha
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ERN1 using Anti-IRE alpha ERN1 Monoclonal Antibody (M00683).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain tissue (A) and stomach tissue (B)Click image to see more details
Figure 1. Western blotting validation for Anti-IRE alpha ERN1 Monoclonal Antibody M00683
Western Blot (WB) analysis using IRE1alpha Monoclonal Antibody against Raji (1)Protein Target Info & Infographic
Gene/Protein Information For ERN1 (Source: Uniprot.org, NCBI)
Gene Name
ERN1
Full Name
Serine/threonine-protein kinase/endoribonuclease IRE1
Weight
109.735kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; FLJ30999; hIRE1p; inositol-requiring 1; Inositol-requiring protein 1; IRE 1a; ire1 alpha p Ser724; ire1 alpha phosphorylation; IRE1a; ire1-alpha; IRE1MGC163279; IRE1P; MGC163277; phospho ire1 alpha; phospho-ire1 alpha; phospho-ire1a; p-ire1 alpha; protein kinase/endoribonuclease; serine/threonine-protein kinase/endoribonuclease IRE1 ERN1 IRE1, IRE1P, IRE1a, hIRE1p endoplasmic reticulum to nucleus signaling 1 serine/threonine-protein kinase/endoribonuclease IRE1|ER to nucleus signalling 1|inositol-requiring 1|inositol-requiring enzyme 1|inositol-requiring protein 1|ire1-alpha|protein kinase/endoribonuclease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERN1, check out the ERN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRE alpha ERN1 Monoclonal Antibody (M00683)
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