Rabbit IgG polyclonal antibody for X-ray repair cross-complementing protein 5(XRCC5) detection. Tested with WB, IHC-P, ICC in Human.
Anti-Ku80/XRCC5 Antibody Info At A Glance
|Applications:||IHC, ICC, WB|
|Product Name||Anti-Ku80/XRCC5 Antibody
See all Ku80/XRCC5 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Ku80/XRCC5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Ku80/XRCC5 information: Molecular Weight: 82705 MW; Subcellular Localization: Nucleus. Nucleus, nucleolus. Chromosome.|
|Cite This Product||Anti-Ku80/XRCC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1641)|
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|Anti-Ku80/XRCC5 Antibody may replace the following items: sc 135964|sc 1484|sc 1485|sc 33653|sc 5280|sc 53021|sc 56135|sc 56136|sc 65575|sc 71471|sc 9034.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80(648-662aa KFSEEQRFNNFLKAL).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For XRCC5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7520|
|Species Of This Entry||Human|
|Protein Name||X-ray repair cross-complementing protein 5|
|Alternative Names||Ku80/XRCC5|80kDa; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC85; CTCBF; EC 3.6.4.-; FLJ39089; G22P2; KARP1; KARP-1; Ku80; Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen; Ku86; Ku86DNA repair protein XRCC5; KUB2; NFIV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Demethylation; Acetylation; Reduction; Oxidation; Biotinylation; Phosphorothioation; Ribosylation|
|Subcellular Localization||Nucleus. Nucleus, nucleolus. Chromosome.|
|Gene Location||2q35, on Chromasome 2, gene sequence: NC_000002.12 (216109348..216206293)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For XRCC5 (Source: Uniprot.org, NCBI)
|Protein Function||Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose- 5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). Plays a role in the regulation of DNA virus- mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.|
|Interacting Proteins||APLF; COIL; GOLPH3; HOXB7; HSPB1; PPM1G; PRKDC; TPT1; XRCC6|
|Research Areas||Cancer; Chromatin Research; Core ESC-Like Genes; DNA Double Strand Break Repair; DNA Repair; Non-homologous end-joining; Stem Cell Markers|
|Related Diseases||Neoplasms; Malignant Neoplasms; Autoimmune Reaction; Severe Combined Immunodeficiency; Carcinoma; Lupus Erythematosus, Systemic; Autoimmune Diseases; Immunologic Deficiency Syndromes; Genomic Instability; Cell Transformation, Neoplastic; Malignant Paraganglionic Neoplasm; Ataxia Telangiectasia; Chromosome Breakage; Mammary Neoplasms; Cytogenetic Abnormality; Leukemia; Diffuse Scleroderma; Werner Syndrome; Ataxia; Lymphoma|
|Related Pathways||Dna Repair; V(d)j Recombination; Double-strand Break Repair; Cell Cycle; Localization; Dna Replication; Telomere Maintenance; Cell Death; Cell Proliferation; Aging; Senescence; S Phase; Hypersensitivity; Interphase; Recombinational Repair; Pathogenesis; Cell Growth; Immune Response; Dna Recombination; Cell Cycle Arrest|
|Background||XRCC5(X-ray Repair, Complementing Defective, In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene. The XRCC5 gene encodes the 80-kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining(NHEJ) pathway. The XRCC5 gene is mapped to 2q35. Human colon cancer cells heterozygous for Ku86 are haploinsufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation. Functional inactivation of the second Ku86 allele results in cells with a drastically reduced doubling time. The Ku86 locus is essential in human somatic tissue culture cells by experiments demonstration. A rare microsatellite polymorphism in XRCC5 is associated with cancer in patients of varying radiosensitivity.|
|Scientific References||PMID: 2760028 by Yaneva M., et al. cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.|
PMID: 2308937 by Mimori T., et al. Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome.
Our Boster Quality Guarantee for Anti-Ku80/XRCC5 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Ku80 (PA1641) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
IHC(P): Human Mammary Cancer Tissue
ICC: HELA Cell
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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