Anti-Ku80/XRCC5 Antibody

Ku80/XRCC5 antibody

Boster Bio Anti-Ku80/XRCC5 Antibody catalog # PA1641. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1641
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Ku80/XRCC5 Antibody

View all Ku80/XRCC5 Antibodies

SKU/Catalog Number

PA1641

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ku80/XRCC5 Antibody catalog # PA1641. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ku80/XRCC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1641)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1641 is reactive to XRCC5 in Human

Applications

PA1641 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

83 kDa

Calculated molecular weight

82.705kDa

Background of Ku80/XRCC5

XRCC5 (X-ray Repair, Complementing Defective, In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene. The XRCC5 gene encodes the 80-kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining (NHEJ) pathway. The XRCC5 gene is mapped to 2q35. Human colon cancer cells heterozygous for Ku86 are haploinsufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation. Functional inactivation of the second Ku86 allele results in cells with a drastically reduced doubling time. The Ku86 locus is essential in human somatic tissue culture cells by experiments demonstration. A rare microsatellite polymorphism in XRCC5 is associated with cancer in patients of varying radiosensitivity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunofluorescence, 2μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For XRCC5 (Source: Uniprot.org, NCBI)

Gene Name

XRCC5

Full Name

X-ray repair cross-complementing protein 5

Weight

82.705kDa

Superfamily

ku80 family

Alternative Names

80kDa; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC85; CTCBF; EC 3.6.4.-; FLJ39089; G22P2; KARP1; KARP-1; Ku80; Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen; Ku86; Ku86DNA repair protein XRCC5; KUB2; NFIV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing XRCC5 KARP-1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair cross complementing 5 X-ray repair cross-complementing protein 5|86 kDa subunit of Ku antigen|ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku autoantigen, 80kDa|Ku86 autoantigen related protein 1|TLAA|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|lupus Ku autoantigen protein p86|nuclear factor IV|thyroid-lupus autoantigen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on XRCC5, check out the XRCC5 Infographic

XRCC5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for XRCC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Ku80/XRCC5 Antibody

Question

My team were content with the WB result of your anti-Ku80/XRCC5 antibody. However we have seen positive staining in neuroblastoma nucleus. using this antibody. Is that expected? Could you tell me where is XRCC5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

According to literature, neuroblastoma does express XRCC5. Generally XRCC5 expresses in nucleus. Regarding which tissues have XRCC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8605992
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, and Osteoblast, Pubmed ID: 12145306
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Neuroblastoma, Pubmed ID: 14702039
Thyroid, and Uterus, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-25

Question

I was wanting to use using your anti-Ku80/XRCC5 antibody for nonhomologous end-joining (nhej) studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-08-16

Answer

Thank you for your inquiry. This PA1641 anti-Ku80/XRCC5 antibody is tested on jurkat whole cell lysate, cem whole cell lysate, raji whole cell lysate, colo320 whole cell lysate, ht1080 whole cell lysate, mammary cancer tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-08-16

Question

We are currently using anti-Ku80/XRCC5 antibody PA1641 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-21

Answer

The anti-Ku80/XRCC5 antibody (PA1641) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-21

Question

We have been able to see staining in human neuroblastoma. Do you have any suggestions? Is anti-Ku80/XRCC5 antibody supposed to stain neuroblastoma positively?

Verified Customer

Verified customer

Asked: 2017-09-14

Answer

Based on literature neuroblastoma does express XRCC5. Based on Uniprot.org, XRCC5 is expressed in kidney, neuroblastoma, coronary artery, thyroid uterus, cervix carcinoma, heart osteoblast, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have XRCC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8605992
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, and Osteoblast, Pubmed ID: 12145306
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Neuroblastoma, Pubmed ID: 14702039
Thyroid, and Uterus, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-09-14

Question

Our lab used your anti-Ku80/XRCC5 antibody for WB on coronary artery a few months ago. I am using human, and We want to use the antibody for ICC next. We are interested in examining coronary artery as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

J. Banerjee

Verified customer

Asked: 2017-01-23

Answer

I have checked the website and datasheets of our anti-Ku80/XRCC5 antibody and it appears that PA1641 has been validated on human in both WB and ICC. Thus PA1641 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-01-23

Order DetailsPrice
PA1641

100μg

$370
PA1641-10ug

10μg sample (liquid)

$99
PA1641-Biotin

100 μg Biotin conjugated

$570
PA1641-Cy3

100 μg Cy3 conjugated

$570
PA1641-Dylight488

100 μg Dylight488 conjugated

$570
PA1641-Dylight550

100 μg Dylight550 conjugated

$570
PA1641-Dylight594

100 μg Dylight594 conjugated

$570
PA1641-FITC

100 μg FITC conjugated

$570
PA1641-HRP

100 μg HRP conjugated

$570
PA1641-APC

100 μg APC conjugated

$670
PA1641-PE

100 μg PE conjugated

$670
PA1641-carrier-free

Carrier Free

$370

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