Anti-LXR-A NR1H3 Antibody

Product Name

Anti-LXR-A NR1H3 Antibody

View all NR1H3 Antibodies

SKU/Catalog Number

A03331

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LXR-A NR1H3 Antibody (Catalog # A03331). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LXR-A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LXR-A NR1H3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03331)

Host

Rabbit

Contents

LXR-A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LXR-A antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus human LXR-A. The immunogen is located within amino acids 50 - 100 of LXR-A.

Reactive Species

A03331 is reactive to NR1H3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.4 kDa

Background of NR1H3

LXR-A belongs to the Liver X Receptor family that encodes highly homologous transcription factors. Like the highly homologous LXR-B, LXR-A forms heterodimers with the retinoic acid receptor RXRalpha, function as sensors for cellular oxysterols which when activated, increase the expression of genes that control sterol and fatty acid metabolism and homeostasis. Recent experiments have indicated that the LXRs can also modulate both innate and adaptive immune responses. Human and mouse tumors produce LXR ligands that inhibit CCR7 expression on maturing dendritic cells (DCs), thereby allowing tumor immunoescape. In mouse models, it was shown that ablating LXR-A signaling led to an immune-mediated strong inhibition of tumor growth, suggesting that manipulation of this pathway may be a viable anti-cancer approach.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03331 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LXR-A antibody can be used for detection of LXR-A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NR1H3

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