Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM6(MCM6) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Anti-MCM6 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC-P, IHC-F, ICC, WB|
|Product Name||Anti-MCM6 Antibody
See all MCM6 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MCM6 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MCM6 information: Molecular Weight: 92889 MW; Subcellular Localization: Nucleus. Binds to chromatin during G1 and detach from it during S phase.|
|Cite This Product||Anti-MCM6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1769)|
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|Anti-MCM6 Antibody may replace the following items: sc 22781|sc 393618|sc 9845|sc 55577|sc 55576|sc 135983|sc 9843|sc 393463.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human MCM6(589-605aa ESEDFIVEQYKHLRQRD), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MCM6 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4175|
|Species Of This Entry||Human|
|Protein Name||DNA replication licensing factor MCM6|
|Alternative Names||MCM6|DNA replication licensing factor MCM6; EC 188.8.131.52; MCG40308; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S.cerevisiae); MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); minichromosome maintenance complex component 6; minichromosome maintenance deficient (mis5, S. pombe) 6; minichromosome maintenance deficient 6 homolog (S. cerevisiae); minichromosome maintenance deficient 6 homolog; MIS5 homolog; Mis5; p105MCM|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Carboxylation; Methylation; Dephosphorylation|
|Subcellular Localization||Nucleus. Binds to chromatin during G1 and detach from it during S phase.|
|Gene Location||2q21.3, on Chromasome 2, gene sequence: NC_000002.12 (135839626..135876443, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MCM6 (Source: Uniprot.org, NCBI)
|Protein Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.|
|Interacting Proteins||CDKN2A; CDT1; FAM161A; MCM10; MCM2; MCM3; MCM7; MCMBP; NUDT2; SNRPB2; SSRP1; ZBTB9|
|Research Areas||Cell Cycle and Replication; DNA Repair|
|Related Diseases||Neoplasms; Malignant Neoplasms; Lactose Intolerance; Retinoblastoma; Carcinoma; Cell Transformation, Neoplastic; Lung Neoplasms; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Lung; Melanoma; Non-small Cell Lung Carcinoma; Dysplasia; Lymphoma; Adenocarcinoma; Epithelioma; Craniopharyngioma; Vascular Diseases; Hyperplasia; Papillary Neoplasm; Genomic Instability|
|Related Pathways||Dna Replication; Cell Cycle; S Phase; Localization; Cell Proliferation; Cell Division; Mitosis; G1 Phase; Cell Growth; Dna Replication Initiation; Transport; Cell Cycle Arrest; Regulation Of Dna Replication; Interphase; Dna Repair; Pathogenesis; Translation; Cell Death; Single-stranded Dna Binding; M Phase|
|Background||MCM6(Minichromosome maintenance, s. pombe, homolog of, 6) is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM genes were originally identified in yeast defective in minichromosome maintenance and have since been shown to play roles in the progression of the cell cycle; many are cell division control genes. The MCM6 gene is mapped on 2q21.3. Mcm 6 has recently been shown to interact strongly Cdt1 at defined residues, by mutating these target residues Wei et al. observed lack of Cdt1 recruitment of Mcm2-7 to the pre-RC. An approximately 200-kb region surrounding the C/T(-13910) polymorphism in MCM6 intron 13 functioned as an enhancer of the lactase gene promoter in intestinal cell culture.|
|Scientific References||PMID: 9286856 by Tsuruga H., et al. HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5.|
PMID: 9516426 by Holthoff H.P., et al. Human protein MCM6 on HeLa cell chromatin.
Our Boster Quality Guarantee for Anti-MCM6 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: JURKAT Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Rat Brain Tissue
ICC: HELA Cell
IHC(F): Human Placenta Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates,
Lane 2: mouse spleen tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- MCM6 antigen affinity purified polyclonal antibody (Catalog # PA1769) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM6 at approximately 93KD. The expected band size for MCM6 is at 93KD.
MCM6 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- MCM6 Antibody (PA1769) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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