Mmp9 antibody (PB9669) performs well in IHC, WB and shows reactivity with Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 63 Publications
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Anti-MMP9 Antibody Picoband®

Mmp9 antibody

Reactive SpeciesHostApplication
Mouse, RatRabbitIHC, WB

SKU: PB9669

Size: 100 μg

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Price:
$370/100 μg

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Guarantee / Product Promise

Tested Applications

Mmp9 antibody was positively detected by WB in:

NRK Whole Cell, ANA-1 Whole Cell, HEPA Whole Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Mmp9 antibody was positively detected by IHC in:

mouse kidney tissue, rat liver tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Recommend Dilution

Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
Mouse, -
Western blot
0.1-0.5μg/ml
Mouse, Rat

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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Boster Guarantee:What is this?
IHC
IF
Mouse
Tested
Recommend
Rat
Tested
Recommend

Product Information

Product Name
Anti-MMP9 Antibody Picoband®
View all MMP9 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse MMP-9, different from the related human sequence by thirteen amino acids, and from the related rat sequence by eight amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated Molecular Weight

80.5 kDa

Background of Mmp9
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Cite This Product
Anti-MMP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9669)

Protocols

Western Blot protocol for Mmp9 antibody
Immunohistochemistry (IHC) protocol for Mmp9 antibody

Publications

Application:
Species:
TRAP5 Inhibition Targeting Scar-Associated Macrophages Ameliorates Acute Kidney Injury to Chronic Kidney Disease Transition
Published: 2026-03-03
Journal: Advanced Science
Application: IF
Impact Factor: 14.10
Species: Mouse,Human
PMID: 41773735
Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation
Published: 2026-02-21
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 8.30
Species: Mouse
PMID: 41759424
Gut microbiota dysbiosis impairs TGF-β/Smad4 signaling to drive postoperative metastasis in colorectal cancer
Published: 2025-12-15
Journal: Frontiers in Microbiology
Application: IHC
Impact Factor: 4.50
Species: Mouse
PMID: 41472816
Targeted Neuroimmune Modulation via FGF21-Loaded Dual-Layer Electrospun Nanofibrous Scaffold to Suppress Secondary Injury After Severe Traumatic Brain Injury
Published: 2025-06-25
Journal: Advanced Healthcare Materials
Application: WB
Impact Factor: 9.60
Species: Human
PMID: 40557530
Neutrophil Membrane-Engineered Extracellular Vesicles from Perivascular Adipose Tissue-Derived Stromal Cells for Atherosclerotic Lesion Resolution and Enhanced Vascular Graft Remodeling
Published: 2025-06-02
Journal: ADVANCED FUNCTIONAL MATERIALS
Application: IHC
Impact Factor: 19.00
Species: Rat
Apigenin prevents hypertensive vascular remodeling by regulating the TP53 pathway
Published: 2025-05-02
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Rat
PMID: 40315632
Downregulation of GFPT2 enhances cisplatin chemotherapy sensitivity in STK11/KRAS mutant non-small cell lung cancer by regulating the hexosamine biosynthesis pathway, resisting tumor growth
Published: 2025-04-19
Journal: CYTOKINE
Application: WB
Impact Factor: 3.70
Species: Mouse
PMID: 40253947
Mesenchymal Stem Cells Restore Endothelial Integrity and Alleviate Emotional Impairments in a Diabetic Mouse Model via Inhibition of MMP-9 Activity
Published: 2025-04-03
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.90
Species: Mouse
PMID: 40244194
Investigating the molecular mechanism of epimedium herb in treating rheumatoid arthritis through network pharmacology, molecular docking, and experimental validation
Published: 2025-01-16
Journal: MOLECULAR DIVERSITY
Application: IHC
Impact Factor: 3.80
Species: Mouse
PMID: 39821498
Orcinol gentiobioside inhibits RANKL-induced osteoclastogenesis by promoting apoptosis and suppressing autophagy via the JNK1 signaling
Published: 2024-03-21
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38521429
Sesamin attenuates UVA-induced keratinocyte injury via inhibiting ASK-1-JNK/p38 MAPK pathways
Published: 2023-08-06
Journal: Journal of Cosmetic Dermatology
Application: WB
Impact Factor: 2.30
Species: Human
PMID: 37545137
Evaluation of the therapeutic effects of oestradiol on the systemic inflammatory response and on lung injury caused by the occlusion of the proximal descending aorta in male rats
Published: 2023-07-06
Journal: EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
Application: IHC
Impact Factor: 3.40
Species: Rat
PMID: 37410160
Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis
Published: 2023-02-18
Journal: PHYTOTHERAPY RESEARCH
Application: IHC
Impact Factor: 7.20
Species: Rat
PMID: 36807664
3D bioprinting of heterogeneous tissue-engineered skin containing human dermal fibroblasts and keratinocytes
Published: 2023-02-02
Journal: Biomaterials Science
Application: IF
Impact Factor: 6.60
Species: Human
PMID: 36762551
Effect and mechanism of safranal on ISO-induced myocardial injury based on network pharmacology
Published: 2022-12-29
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.20
Species: Rat
PMID: 36586525
CYP1B1: A Novel Molecular Biomarker Predicts Molecular Subtype, Tumor Microenvironment, and Immune Response in 33 Cancers
Published: 2022-11-17
Journal: Cancers
Application: IHC
Impact Factor: 6.58
Species: Human
PMID: 36428734
Jin-Tian-Ge ameliorates ovariectomy-induced bone loss in rats and modulates osteoblastogenesis and osteoclastogenesis in vitro
Published: 2022-10-05
Journal: Chinese Medicine
Application: WB
Impact Factor: 4.55
Species: Mouse
PMID: 36195960
Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway
Published: 2022-09-24
Journal: Nutrients
Application: WB
Impact Factor: 6.71
Species: Mouse
PMID: 36235631
Chronic exposure to microcystin-leucine-arginine induces epithelial hyperplasia and inflammation in the mouse bladder
Published: 2022-09-05
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB,IHC
Impact Factor: 7.13
Species: Mouse
PMID: 36075121
Microglia Regulate Blood–Brain Barrier Integrity via MiR-126a-5p/MMP9 Axis during Inflammatory Demyelination
Published: 2022-06-27
Journal: Advanced Science
Application: IF,WB
Impact Factor: 17.52
Species: Mouse, Human
PMID: 35758549
Curcumin Administered in Combination with Glu-GNPs Induces Radiosensitivity in Transplanted Tumor MDA-MB-231-luc Cells in Nude Mice
Published: 2021-11-16
Journal: Biomed Research International
Application: IHC
Impact Factor: 3.41
Species: Mouse
PMID: 34825004
Cardioprotective effects of the proline-rich oligopeptide Bj-PRO-7a in spontaneously hypertensive rats
Published: 2021-08-24
Journal: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
Application: WB
Impact Factor: 2.56
Species: Rat
PMID: 34427931
The number and periodicity of seizures induce cardiac remodeling and changes in micro-RNA expression in rats submitted to electric amygdala kindling model of epilepsy
Published: 2021-02-03
Journal: EPILEPSY & BEHAVIOR
Application: WB
Impact Factor: 2.94
Species: Rat
PMID: 33548915
HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway
Published: 2020-05-15
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC,WB
Impact Factor: 3.26
Species: Rat
PMID: 32417325
Employing the Sirolimus-Eluting Poly (Propylene Carbonate) Mesh for the Prevention of Arteriovenous Graft Stenosis in Rats
Published: 2019-05-01
Journal: JOURNAL OF CARDIOVASCULAR PHARMACOLOGY AND THERAPEUTICS
Application: WB
Impact Factor: 2.57
Species: Rat
PMID: 30474386
Synthesis and anti-metastasis activities of norcantharidin-conjugated carboxymethyl chitosan as a novel drug delivery system
Published: 2019-03-11
Journal: CARBOHYDRATE POLYMERS
Application: IHC
Impact Factor: 6.04
Species: Mouse
PMID: 30926011
Reversal effect of Zhigancao decoction on myocardial fibrosis in a rapid pacing-induced atrial fibrillation model in New Zealand rabbits.
Published: 2019-01-11
Journal: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
Application: IHC
Impact Factor: 1.35
Species: Rabbit
PMID: 30632430
Bone marrow mesenchymal stem cells attenuate the progression of focal segmental glomerulosclerosis in rat models
Published: 2018-11-22
Journal: BMC Nephrology
Application: IHC
Impact Factor: 2.40
Species: Rat
PMID: 30466397
Anti-endometriosis Mechanism of Jiawei Foshou San Based on Network Pharmacology
Published: 2018-07-26
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 3.83
Species: Rat
PMID: 30093862
Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats
Published: 2018-02-01
Journal: Molecular Medicine Reports
Application: IHC
Impact Factor: 1.92
Species: Mouse
PMID: 29393453
Cardioprotection of Sheng Mai Yin a classic formula on adriamycin induced myocardial injury in Wistar rats
Published: 2017-10-25
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 3.53
Species: Rat
PMID: 29425641
Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats
Published: 2017-10-04
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.69
Species: Rat
PMID: 28990064
IMM-H004, A New Coumarin Derivative, Improved Focal Cerebral Ischemia via Blood–Brain Barrier Protection in Rats
Published: 2017-06-29
Journal: Journal of Stroke & Cerebrovascular Diseases
Application: IHC
Impact Factor: 1.52
PMID: 28669655
AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE−/− mice
Published: 2017-05-27
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: IHC
Impact Factor: 2.47
Species: Mouse
PMID: 28559142
Inhibitory effects of doxycycline on the onset and progression of abdominal aortic aneurysm and its related mechanisms
Published: 2017-05-22
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 2.90
Species: Rat
PMID: 28545777
Inhibition of glioblastoma growth and invasion by 125I brachytherapy in rat glioma model
Published: 2017-05-15
Journal: American Journal of Translational Research
Application: WB
Impact Factor: 2.83
Species: Rat
PMID: 28559975
Effects of the Traditional Chinese Medicine Dilong on Airway Remodeling in Rats with OVA-induced-Asthma
Published: 2016-12-16
Journal: Open Life Sciences
Impact Factor: 0.00
Species: Rat
Effects of electromagnetic pulse exposure on gelatinase of blood–brain barrier in vitro
Published: 2016-06-29
Journal: ELECTROMAGNETIC BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 1.21
Species: Rat
PMID: 27355558
Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway
Published: 2016-06-06
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 2.55
Species: Rat
PMID: 27280587
Fentanyl inhibits the progression of human gastric carcinoma MGC-803 cells by modulating NF-κB-dependent gene expression in vivo
Published: 2016-05-25
Journal: Oncology Letters
Application: IHC
Impact Factor: 1.48
Species: Mouse
PMID: 27347181
Targeted delivery of let-7b to reprogramme tumor-associated macrophages and tumor infiltrating dendritic cells for tumor rejection
Published: 2016-03-09
Journal: BIOMATERIALS
Application: IF,IHC
Impact Factor: 8.39
Species: Mouse
PMID: 26994345
Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway
Published: 2016-02-26
Journal: PLoS One
Application: WB
Impact Factor: 3.06
Species: Rat
PMID: 26918963
Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Published: 2016-01-18
Journal: ACTA PHARMACOLOGICA SINICA
Application: WB
Impact Factor: 3.17
Species: Rat
PMID: 26775665
Effect of Ginkgo biloba extract on experimental cardiac remodeling
Published: 2015-08-13
Journal: BMC Complementary and Alternative Medicine
Application: IHC
Impact Factor: 2.02
Species: Rat
PMID: 26268459
Targeted depletion of tumour-associated macrophages by an alendronate–glucomannan conjugate for cancer immunotherapy
Published: 2014-09-22
Journal: BIOMATERIALS
Application: IF
Impact Factor: 8.31
PMID: 25245263
Expression of matrix metalloproteinases‑8 and ‑9 and their tissue inhibitor in the condyles of diabetic rats with mandibular advancement
Published: 2014-09-22
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 0.94
Species: Rat
PMID: 25289023
Rapamycin alleviates brain edema after focal cerebral ischemia reperfusion in rats
Published: 2014-05-22
Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
Application: IHC
Impact Factor: 1.11
Species: Rat
PMID: 24773551
In vitro and in vivo study of dolichyl phosphate on the efflux activity of P-glycoprotein at the blood–brain barrier
Published: 2013-10-22
Journal: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
Application: WB
Impact Factor: 2.69
Species: Rat
PMID: 24161469
AQP4 KO exacerbating renal dysfunction is mediated by endoplasmic reticulum stress and p66Shc and is attenuated by apocynin and endothelin antagonist CPU0213
Published: 2013-10-14
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 2.59
Species: Mouse
PMID: 24135202
Alteration in P-glycoprotein at the blood–brain barrier in the early period of MCAO in rats
Published: 2013-02-26
Journal: JOURNAL OF PHARMACY AND PHARMACOLOGY
Application: WB
Impact Factor: 2.03
Species: Rat
PMID: 23600383
The effect of 2,3,4′,5-tetrahydroxystilbene-2-0-β-d glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms
Published: 2012-11-21
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 2.52
Species: Rat
PMID: 23178522
Overexpressed C-type natriuretic peptide serves as an early compensatory response to counteract extracellular matrix remodeling in unilateral ureteral obstruction rats
Published: 2012-10-17
Journal: MOLECULAR BIOLOGY REPORTS
Application: IHC
Impact Factor: 2.93
Species: Rat
PMID: 23073778
Erythromycin ameliorates cigarette-smoke–induced emphysema and inflammation in rats
Published: 2011-10-23
Journal: Translational Research
Application: IHC
Impact Factor: 2.90
Species: Rat
PMID: 22633097
Sildenafil improves diabetic vascular activity through suppressing endothelin receptor A, iNOS and NADPH oxidase which is comparable with the endothelin receptor antagonist CPU0213 in STZ-injected rats
Published: 2011-06-02
Journal: JOURNAL OF PHARMACY AND PHARMACOLOGY
Application: WB
Impact Factor: 1.92
Species: Rat
PMID: 21635260
rAAV2-TGF-β3 Decreases Collagen Synthesis and Deposition in the Liver of Experimental Hepatic Fibrosis Rat
Published: 2010-01-28
Journal: DIGESTIVE DISEASES AND SCIENCES
Application: IHC
Impact Factor: 1.84
Species: Rat
PMID: 20108118
Strontium fructose 1,6-diphosphate alleviates early diabetic testopathy by suppressing abnormal testicular matrix metalloproteinase system in streptozocin-treated rats
Published: 2010-01-08
Journal: JOURNAL OF PHARMACY AND PHARMACOLOGY
Application: WB
Impact Factor: 1.74
Species: Rat
PMID: 19178771
Inhibition of Calcium–Calmodulin-Dependent Kinase II Suppresses Cardiac Fibroblast Proliferation and Extracellular Matrix Secretion
Published: 2010-01-01
Journal: JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
Application: WB
Impact Factor: 2.83
Species: Rat
PMID: 19935079
Effect of total saponins of “panax notoginseng root” on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats
Published: 2009-09-11
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 2.33
Species: Rat
PMID: 19748258
Antitumor and antimetastatic activities of Rhizoma Paridis saponins
Published: 2009-08-20
Journal: STEROIDS
Application: IHC
Impact Factor: 2.59
Species: Mouse
PMID: 19699217
Isoproterenol disperses distribution of NADPH oxidase, MMP-9, and pPKCε in the heart, which are mitigated by endothelin receptor antagonist CPU0213
Published: 2009-07-13
Journal: ACTA PHARMACOLOGICA SINICA
Application: WB
Impact Factor: 1.68
Species: Rat
PMID: 19597524
Adenovirus-mediated transfer of siRNA against PAI-1 mRNA ameliorates hepatic fibrosis in rats
Published: 2009-04-10
Journal: JOURNAL OF HEPATOLOGY
Application: IHC
Impact Factor: 7.06
Species: Rat
PMID: 19446913
2,3,4′,5-TETRAHYDROXYSTILBENE-2-O-β-d-GLUCOSIDE SUPPRESSES MATRIX METALLOPROTEINASE EXPRESSION AND INFLAMMATION IN ATHEROSCLEROTIC RATS
Published: 2007-10-31
Journal: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
Application: IHC
Impact Factor: 1.78
Species: Rat
PMID: 17973930
A New ATP-Sensitive Potassium Channel Opener Protects the Kidney from Hypertensive Damage in Spontaneously Hypertensive Rats
Published: 2005-11-01
Journal: JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Application: IHC
Impact Factor: 4.34
Species: Rat
PMID: 16051697

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We promise Anti-MMP9 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

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PB9669
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 2, 2026