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MMP9 antibody (PA1357) performs well in WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 67 Publications

Anti-MMP-9 antibody, PA1357, Western blotting
Lane 1: Rat Embryo Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: HT1080 Cell Lysate

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2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

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Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband®

MMP9 antibody

67 Publications

Reactive Species	Host	Application
Human	Rabbit	WB

Tested Applications

MMP9 antibody was positively detected by WB in:

Rat Embryo Tissue, MM453 Cell, HELA Cell, SMMC Cell, JURKAT Cell, HT1080 Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

Rat

Tested

Human

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband®
View all MMP9 Antibodies

Form

Lyophilized

Uniprot

P14780

Gene ID

4318

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

60 kDa

Calculated Molecular Weight

78.5 kDa

Background of MMP9

Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Cite This Product

Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1357)

Protocols

Western Blot protocol for MMP9 antibody

Review Protocol

Publications

Li CH,Liu M,Pan LH,Sun Y.ANP reduced Hedgehog signaling-mediated activation of matrix metalloproteinase-9 in gastric cancer cell line MGC-803.Gene.2020 Dec 15;762:145044.doi:10.1016/j.gene.2020.145044.Epub 2020 Aug 7.PMID:32777528.

Species: Human

PMID: 32777528

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Free Secondary Antibody

Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband®

MMP9 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Li CH,Liu M,Pan LH,Sun Y.ANP reduced Hedgehog signaling-mediated activation of matrix metalloproteinase-9 in gastric cancer cell line MGC-803.Gene.2020 Dec 15;762:145044.doi:10.1016/j.gene.2020.145044.Epub 2020 Aug 7.PMID:32777528.

The impact of chronic endometritis on endometrial fibrosis and reproductive prognosis in patients with moderate and severe intrauterine adhesions: a prospective cohort study Lixiang Liu, M.D., et al. Fertil Steril. 2019 May;111(5):1002-1010.e2. doi: 10

Ketamine inhibits lipopolysaccharide-induced astrocytes activation by suppressing TLR4/NF-%u0138B pathway

1-calcium phosphate-uracil, a synthesized pyrimidine derivative agent, has anti-proliferative, pro-apoptotic and anti-invasion effects on multiple tumor cell lines

MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/?-catenin pathway

Main components of pomegranate, ellagic acid and luteolin, inhibit metastasis of ovarian cancer by down-regulating MMP2 and MMP9

Early changes in the apparent diffusion coefficient and MMP-9 expression of a cervical carcinoma U14 allograft model following irradiation

Mesenchymal stem cells overexpressing adrenomedullin improve heart function through antifibrotic action in rats experiencing heart failure

Adipose differentiation-related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation

Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats

Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation

Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization

Preventive Effects of Rhodiola rosea L on Bleomycin-Induced Pulmonary Fibrosis in Rats

Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer

Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43

Isolation and characteristics of CD133?/A2B5+ and CD133?/A2B5? cells from the SHG139s cell line

Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo

Effect of Ginkgo biloba extract on experimental cardiac remodeling

Expression of matrix metalloproteinases-8 and -9 and their tissue inhibitor in the condyles of diabetic rats with mandibular advancement

Huoxue Rongluo?Tablet reduces matrix metalloproteinase-9 expression in infarcted brain tissue

Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma

Yang F, Luo L, Zhu ZD, Zhou X, Wang Y, Xue J, Zhang J, Cai X, Chen ZL, Ma Q, Chen YF, Wang YJ, Luo YY, Liu P, Zhao L. Front Pharmacol. 2017 Dec 19;8:929. doi: 10.3389/fphar.2017.00929. eCollection 2017. Chlorogenic Acid Inhibits Liver Fibrosis by ...

Zhang Y, Shen Y, Cao B, Yan A, Ji H. Exp Ther Med. 2015 Mar;9(3):905-908. Epub 2014 Dec 19. Elevated Expression Levels Of Androgen Receptors And Matrix Metalloproteinase-2 And -9 In 30 Cases Of Hepatocellular Carcinoma Compared With Adjacent Tissu...

Sun Y, Liu M, Yang B, Lu J, Li B. Cancer Invest. 2008 Dec;26(10):984-9. Doi: 10.1080/07357900802072897. Inhibition Of Laryngeal Cancer Cell Invasion And Growth With Lentiviral-Vector Delivered Short Hairpin Rna Targeting Human Mmp-9 Gene.

Meng L, Yu B. Int J Mol Med. 2011 Nov;28(5):777-83. Doi: 10.3892/Ijmm.2011.744. Epub 2011 Jul 8. Oxygen- And Glucose-Deprived Culture Promotes Cell Proliferation And Invasion Of Vascular Smooth Muscle Cells.

Wu G, Wang L, Hong Z, Li C, Long X, Sheng F. Neurol Res. 2011 Apr;33(3):300-6. Doi: 10.1179/016164110X12759951866993. Epub 2010 Aug 16. Effects Of Minimally Invasive Procedures For Removal Of Intracranial Hematoma On Matrix Metalloproteinase Expre...

Cheng Ys, Dai Dz, Dai Y. Acta Pharmacol Sin. 2009 Aug;30(8):1099-106. Doi: 10.1038/Aps.2009.104. Epub 2009 Jul 13. Isoproterenol Disperses Distribution Of Nadph Oxidase, Mmp-9, And Ppkcepsilon In The Heart, Which Are Mitigated By Endothelin Recept...

Chen Y, Chen Y, Huang L, Yu J. J Dermatol. 2012 Apr;39(4):339-43. Doi: 10.1111/J.1346-8138.2011.01441.X. Epub 2011 Dec 13. Evaluation Of Heparanase And Matrix Metalloproteinase-9 In Patients With Cutaneous Malignant Melanoma.

Rao Ym, Ji M, Chen Ch, Shi Hr. J Huazhong Univ Sci Technolog Med Sci. 2013 Apr;33(2):266-71. Doi: 10.1007/S11596-013-1109-8. Epub 2013 Apr 17. Effect Of Sirna Targeting Mta1 On Metastasis Malignant Phenotype Of Ovarian Cancer A2780 Cells.

Pang B, Fan H, Zhang Iy, Liu B, Feng B, Meng L, Zhang R, Sadeghi S, Guo H, Pang Q. J Neurooncol. 2012 Feb;106(3):543-9. Doi: 10.1007/S11060-011-0710-6. Epub 2011 Oct 8. Hmga1 Expression In Human Gliomas And Its Correlation With Tumor Proliferation...

Yang Tq, Lu Xj, Wu Tf, Ding Dd, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Guo Lc, Zhang Y, Huang Yl, Zhou Yx, Du Zw. Cancer Sci. 2014 Mar;105(3):265-71. Doi: 10.1111/Cas.12351. Epub 2014 Feb 11. Microrna-16 Inhibits Glioma Cell Growth And Invasion T...

Li Xt, Wang Hz, Wu Zw, Yang Tq, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Huang Yl, Zhou Yx, Du Zw. Cell Mol Neurobiol. 2015 Jul;35(5):679-87. Doi: 10.1007/S10571-015-0163-0. Epub 2015 Feb 8. Mir-494-3P Regulates Cellular Proliferation, Invasion, Mi...

Song Y, Zou H, Wang G, Yang H, Xie Z, Bi J. Neural Regen Res. 2012 Jun 15;7(17):1325-30. Doi: 10.3969/J.Issn.1673-5374.2012.17.007. Matrix Metalloproteinase-9 And Tissue Inhibitor Of Metalloproteinase-1 Expression In Early Focal Cerebral Infarctio...

Zhao Q, Xue Y, Liu Zd, Li H, Wang Jf, Li Zj, Wang Ym, Dong P, Xue Ch. J Food Sci. 2010 Nov-Dec;75(9):H280-8. Doi: 10.1111/J.1750-3841.2010.01837.X. Epub 2010 Oct 15. Differential Effects Of Sulfated Triterpene Glycosides, Holothurin A1, And 24-Deh...

Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary...

Liu Ww, Zeng Zy, Wu Ql, Hou Jh, Chen Yy. Otolaryngol Head Neck Surg. 2005 Mar;132(3):395-400. Overexpression Of Mmp-2 In Laryngeal Squamous Cell Carcinoma: A Potential Indicator For Poor Prognosis.

Liu T, Zheng X, Chen J, Wang N, Xiao J, Zhang D, Yin Z, Li W, Chen S. J Huazhong Univ Sci Technolog Med Sci. 2011 Dec;31(6):819-23. Doi: 10.1007/S11596-011-0683-X. Epub 2011 Dec 16. Effect Of Human Cytomegalovirus On Invasive Capability Of Early P...

Fang R, Zhang G, Guo Q, Ning F, Wang H, Cai S, Du J. Cancer Lett. 2013 Jun 1;333(1):66-75. Doi: 10.1016/J.Canlet.2013.01.014. Epub 2013 Jan 22. Nodal Promotes Aggressive Phenotype Via Snail-Mediated Epithelial-Mesenchymal Transition In Murine Mela...

Liu J, Ping W, Zu Y, Sun W. Int J Clin Exp Pathol. 2014 Aug 15;7(9):6040-7. Ecollection 2014. Correlations Of Lysyl Oxidase With Mmp2/Mmp9 Expression And Its Prognostic Value In Non-Small Cell Lung Cancer.

Chen Lx, He Yj, Zhao Sz, Wu Jg, Wang Jt, Zhu Lm, Lin Tt, Sun Bc, Li Xr. Cancer Biol Ther. 2011 Jan 15;11(2):229-35. Epub 2011 Jan 15. Inhibition Of Tumor Growth And Vasculogenic Mimicry By Curcumin Through Down-Regulation Of The Epha2/Pi3K/Mmp Pat...

Shi Ch, Wang Ws, Zhang Zd, Li Cj, Guo Fj, Li F, Chen Am. J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):111-6. Doi: 10.1007/S11596-015-1398-1. Epub 2015 Feb 12. Effect Of Lentivirus-Mediated Upa Silencing On The Proliferation And Apoptosis ...

Zhao Y, Zong Zh, Xu Hm. Gynecol Oncol. 2010 Mar;116(3):563-71. Doi: 10.1016/J.Ygyno.2009.11.015. Rhoc Expression Level Is Correlated With The Clinicopathological Characteristics Of Ovarian Cancer And The Expression Levels Of Rock-I, Vegf, And Mmp9.

Liu Y, Zhou Y, Zhang Xs, Shen Bz. Oncol Lett. 2011 Nov;2(6):1171-1175. Epub 2011 Aug 17. Expression Of Vegf And Mmp-9 And Mri Imaging Changes In Cerebral Glioma.

Zhang Lh, Jia Yl, Lin Xx, Zhang Hq, Dong Xw, Zhao Jm, Shen J, Shen Hj, Li Ff, Yan Xf, Li W, Zhao Yq, Xie Qm. Biochim Biophys Acta. 2013 Aug;1830(8):4148-59. Doi: 10.1016/J.Bbagen.2013.04.008. Epub 2013 Apr 11. Ad-1, A Novel Ginsenoside Derivative,...

Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...

Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.

Cen J, Liu L, Li Ms, He L, Wang Lj, Liu Yq, Liu M, Ji Bs. J Pharm Pharmacol. 2013 May;65(5):665-72. Doi: 10.1111/Jphp.12033. Epub 2013 Feb 26. Alteration In P-Glycoprotein At The Blood-Brain Barrier In The Early Period Of Mcao In Rats.

Hu P, Wang J, Zhao Xq, Hu B, Lu L, Qin Yh. Mol Biol Rep. 2013 Feb;40(2):1429-41. Doi: 10.1007/S11033-012-2186-7. Epub 2012 Oct 17. Overexpressed C-Type Natriuretic Peptide Serves As An Early Compensatory Response To Counteract Extracellular Matrix...

Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...

Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.

Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.

Wu L, Zhang W, Tang Yh, Li H, Chen By, Zhang Gm, Deng Cq. Phytomedicine. 2010 Mar;17(3-4):233-40. Doi: 10.1016/J.Phymed.2009.07.021. Epub 2009 Sep 11. Effect Of Total Saponins Of "Panax Notoginseng Root" On Aortic Intimal Hyperplasia And The Expre...

Zhou Y, Tan X, Kuang W, Liu L, Wan L. Transl Res. 2012 Jun;159(6):464-72. Doi: 10.1016/J.Trsl.2011.09.007. Epub 2011 Oct 24. Erythromycin Ameliorates Cigarette-Smoke-Induced Emphysema And Inflammation In Rats.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.

Jiang Z, Han B, Li H, Li X, Yang Y, Liu W. Carbohydr Polym. 2015 Jul 10;125:53-60. Doi: 10.1016/J.Carbpol.2015.02.039. Epub 2015 Feb 26. Preparation And Anti-Tumor Metastasis Of Carboxymethyl Chitosan.

Chen Sz, Yao Hq, Zhu Sz, Li Qy, Guo Gh, Yu J. Oncol Lett. 2015 Feb;9(2):915-919. Epub 2014 Dec 4. Expression Levels Of Matrix Metalloproteinase-9 In Human Gastric Carcinoma.

Huang Z, Yang Y, Jiang Y, Shao J, Sun X, Chen J, Dong L, Zhang J. Biomaterials. 2013 Jan;34(3):746-55. Doi: 10.1016/J.Biomaterials.2012.09.062. Epub 2012 Oct 27. Anti-Tumor Immune Responses Of Tumor-Associated Macrophages Via Toll-Like Receptor 4 ...

Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Bear...

Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...

Ma X, Ma X, Ma Z, Sun Z, Yu W, Wang J, Li F, Ding J. Evid Based Complement Alternat Med. 2014;2014:710870. Doi: 10.1155/2014/710870. Epub 2014 Oct 15. The Effects Of Uygur Herb Hyssopus Officinalis L. On The Process Of Airway Remodeling In Asthmat...

Wang B, Liang S, Wang Y, Zhu Xq, Gong W, Zhang Hq, Li Y, Xia Cm. Plos Negl Trop Dis. 2015 Jan 15;9(1):E0003434. Doi: 10.1371/Journal.Pntd.0003434. Ecollection 2015. Th17 Down-Regulation Is Involved In Reduced Progression Of Schistosomiasis Fibrosi...