Anti-Matrix metalloproteinase-9 MMP9 Antibody

Boster Bio Anti-Matrix metalloproteinase-9 MMP9 Antibody catalog # PA1357. Tested in WB applications. This antibody reacts with Human. Cited in 67 publication(s).

Product Info Summary

SKU: PA1357
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Matrix metalloproteinase-9 MMP9 Antibody

See all MMP-9 products

SKU/Catalog Number

PA1357

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Matrix metalloproteinase-9 MMP9 Antibody catalog # PA1357. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Matrix metalloproteinase-9 MMP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1357)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9 (689-705aa NQVDQVGYVTYDILQCP), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1357 is reactive to MMP9 in Human

Applications

PA1357 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For MMP9 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14780

Gene ID

4318

Gene Name

MMP9

Full Name

Matrix metalloproteinase-9

Weight

78.458kDa

Superfamily

peptidase M10A family

Alternative Names

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP9, check out the MMP9 Infographic

MMP9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1357 has been cited in 67 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li CH,Liu M,Pan LH,Sun Y.ANP reduced Hedgehog signaling-mediated activation of matrix metalloproteinase-9 in gastric cancer cell line MGC-803.Gene.2020 Dec 15;762:145044.doi:10.1016/j.gene.2020.145044.Epub 2020 Aug 7.PMID:32777528.
Species: Human
PA1357 usage in article: APP:WB, SAMPLE:MGC-803 CELL, DILUTION:1:400

The impact of chronic endometritis on endometrial fibrosis and reproductive prognosis in patients with moderate and severe intrauterine adhesions: a prospective cohort study Lixiang Liu, M.D., et al. Fertil Steril. 2019 May;111(5):1002-1010.e2. doi: 10

Ketamine inhibits lipopolysaccharide-induced astrocytes activation by suppressing TLR4/NF-%u0138B pathway

1-calcium phosphate-uracil, a synthesized pyrimidine derivative agent, has anti-proliferative, pro-apoptotic and anti-invasion effects on multiple tumor cell lines

MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/?-catenin pathway

Main components of pomegranate, ellagic acid and luteolin, inhibit metastasis of ovarian cancer by down-regulating MMP2 and MMP9

Early changes in the apparent diffusion coefficient and MMP-9 expression of a cervical carcinoma U14 allograft model following irradiation

Mesenchymal stem cells overexpressing adrenomedullin improve heart function through antifibrotic action in rats experiencing heart failure

Adipose differentiation-related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation

Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats

Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation

Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization

Preventive Effects of Rhodiola rosea L on Bleomycin-Induced Pulmonary Fibrosis in Rats

Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer

Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43

Isolation and characteristics of CD133?/A2B5+ and CD133?/A2B5? cells from the SHG139s cell line

Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo

Effect of Ginkgo biloba extract on experimental cardiac remodeling

Expression of matrix metalloproteinases-8 and -9 and their tissue inhibitor in the condyles of diabetic rats with mandibular advancement

Huoxue Rongluo?Tablet reduces matrix metalloproteinase-9 expression in infarcted brain tissue

Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma

Yang F, Luo L, Zhu ZD, Zhou X, Wang Y, Xue J, Zhang J, Cai X, Chen ZL, Ma Q, Chen YF, Wang YJ, Luo YY, Liu P, Zhao L. Front Pharmacol. 2017 Dec 19;8:929. doi: 10.3389/fphar.2017.00929. eCollection 2017. Chlorogenic Acid Inhibits Liver Fibrosis by ...

Zhang Y, Shen Y, Cao B, Yan A, Ji H. Exp Ther Med. 2015 Mar;9(3):905-908. Epub 2014 Dec 19. Elevated Expression Levels Of Androgen Receptors And Matrix Metalloproteinase-2 And -9 In 30 Cases Of Hepatocellular Carcinoma Compared With Adjacent Tissu...

Sun Y, Liu M, Yang B, Lu J, Li B. Cancer Invest. 2008 Dec;26(10):984-9. Doi: 10.1080/07357900802072897. Inhibition Of Laryngeal Cancer Cell Invasion And Growth With Lentiviral-Vector Delivered Short Hairpin Rna Targeting Human Mmp-9 Gene.

Meng L, Yu B. Int J Mol Med. 2011 Nov;28(5):777-83. Doi: 10.3892/Ijmm.2011.744. Epub 2011 Jul 8. Oxygen- And Glucose-Deprived Culture Promotes Cell Proliferation And Invasion Of Vascular Smooth Muscle Cells.

Wu G, Wang L, Hong Z, Li C, Long X, Sheng F. Neurol Res. 2011 Apr;33(3):300-6. Doi: 10.1179/016164110X12759951866993. Epub 2010 Aug 16. Effects Of Minimally Invasive Procedures For Removal Of Intracranial Hematoma On Matrix Metalloproteinase Expre...

Cheng Ys, Dai Dz, Dai Y. Acta Pharmacol Sin. 2009 Aug;30(8):1099-106. Doi: 10.1038/Aps.2009.104. Epub 2009 Jul 13. Isoproterenol Disperses Distribution Of Nadph Oxidase, Mmp-9, And Ppkcepsilon In The Heart, Which Are Mitigated By Endothelin Recept...

Chen Y, Chen Y, Huang L, Yu J. J Dermatol. 2012 Apr;39(4):339-43. Doi: 10.1111/J.1346-8138.2011.01441.X. Epub 2011 Dec 13. Evaluation Of Heparanase And Matrix Metalloproteinase-9 In Patients With Cutaneous Malignant Melanoma.

Rao Ym, Ji M, Chen Ch, Shi Hr. J Huazhong Univ Sci Technolog Med Sci. 2013 Apr;33(2):266-71. Doi: 10.1007/S11596-013-1109-8. Epub 2013 Apr 17. Effect Of Sirna Targeting Mta1 On Metastasis Malignant Phenotype Of Ovarian Cancer A2780 Cells.

Pang B, Fan H, Zhang Iy, Liu B, Feng B, Meng L, Zhang R, Sadeghi S, Guo H, Pang Q. J Neurooncol. 2012 Feb;106(3):543-9. Doi: 10.1007/S11060-011-0710-6. Epub 2011 Oct 8. Hmga1 Expression In Human Gliomas And Its Correlation With Tumor Proliferation...

Yang Tq, Lu Xj, Wu Tf, Ding Dd, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Guo Lc, Zhang Y, Huang Yl, Zhou Yx, Du Zw. Cancer Sci. 2014 Mar;105(3):265-71. Doi: 10.1111/Cas.12351. Epub 2014 Feb 11. Microrna-16 Inhibits Glioma Cell Growth And Invasion T...

Li Xt, Wang Hz, Wu Zw, Yang Tq, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Huang Yl, Zhou Yx, Du Zw. Cell Mol Neurobiol. 2015 Jul;35(5):679-87. Doi: 10.1007/S10571-015-0163-0. Epub 2015 Feb 8. Mir-494-3P Regulates Cellular Proliferation, Invasion, Mi...

Song Y, Zou H, Wang G, Yang H, Xie Z, Bi J. Neural Regen Res. 2012 Jun 15;7(17):1325-30. Doi: 10.3969/J.Issn.1673-5374.2012.17.007. Matrix Metalloproteinase-9 And Tissue Inhibitor Of Metalloproteinase-1 Expression In Early Focal Cerebral Infarctio...

Zhao Q, Xue Y, Liu Zd, Li H, Wang Jf, Li Zj, Wang Ym, Dong P, Xue Ch. J Food Sci. 2010 Nov-Dec;75(9):H280-8. Doi: 10.1111/J.1750-3841.2010.01837.X. Epub 2010 Oct 15. Differential Effects Of Sulfated Triterpene Glycosides, Holothurin A1, And 24-Deh...

Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary...

Liu Ww, Zeng Zy, Wu Ql, Hou Jh, Chen Yy. Otolaryngol Head Neck Surg. 2005 Mar;132(3):395-400. Overexpression Of Mmp-2 In Laryngeal Squamous Cell Carcinoma: A Potential Indicator For Poor Prognosis.

Liu T, Zheng X, Chen J, Wang N, Xiao J, Zhang D, Yin Z, Li W, Chen S. J Huazhong Univ Sci Technolog Med Sci. 2011 Dec;31(6):819-23. Doi: 10.1007/S11596-011-0683-X. Epub 2011 Dec 16. Effect Of Human Cytomegalovirus On Invasive Capability Of Early P...

Fang R, Zhang G, Guo Q, Ning F, Wang H, Cai S, Du J. Cancer Lett. 2013 Jun 1;333(1):66-75. Doi: 10.1016/J.Canlet.2013.01.014. Epub 2013 Jan 22. Nodal Promotes Aggressive Phenotype Via Snail-Mediated Epithelial-Mesenchymal Transition In Murine Mela...

Liu J, Ping W, Zu Y, Sun W. Int J Clin Exp Pathol. 2014 Aug 15;7(9):6040-7. Ecollection 2014. Correlations Of Lysyl Oxidase With Mmp2/Mmp9 Expression And Its Prognostic Value In Non-Small Cell Lung Cancer.

Chen Lx, He Yj, Zhao Sz, Wu Jg, Wang Jt, Zhu Lm, Lin Tt, Sun Bc, Li Xr. Cancer Biol Ther. 2011 Jan 15;11(2):229-35. Epub 2011 Jan 15. Inhibition Of Tumor Growth And Vasculogenic Mimicry By Curcumin Through Down-Regulation Of The Epha2/Pi3K/Mmp Pat...

Shi Ch, Wang Ws, Zhang Zd, Li Cj, Guo Fj, Li F, Chen Am. J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):111-6. Doi: 10.1007/S11596-015-1398-1. Epub 2015 Feb 12. Effect Of Lentivirus-Mediated Upa Silencing On The Proliferation And Apoptosis ...

Zhao Y, Zong Zh, Xu Hm. Gynecol Oncol. 2010 Mar;116(3):563-71. Doi: 10.1016/J.Ygyno.2009.11.015. Rhoc Expression Level Is Correlated With The Clinicopathological Characteristics Of Ovarian Cancer And The Expression Levels Of Rock-I, Vegf, And Mmp9.

Liu Y, Zhou Y, Zhang Xs, Shen Bz. Oncol Lett. 2011 Nov;2(6):1171-1175. Epub 2011 Aug 17. Expression Of Vegf And Mmp-9 And Mri Imaging Changes In Cerebral Glioma.

Zhang Lh, Jia Yl, Lin Xx, Zhang Hq, Dong Xw, Zhao Jm, Shen J, Shen Hj, Li Ff, Yan Xf, Li W, Zhao Yq, Xie Qm. Biochim Biophys Acta. 2013 Aug;1830(8):4148-59. Doi: 10.1016/J.Bbagen.2013.04.008. Epub 2013 Apr 11. Ad-1, A Novel Ginsenoside Derivative,...

Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...

Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.

Cen J, Liu L, Li Ms, He L, Wang Lj, Liu Yq, Liu M, Ji Bs. J Pharm Pharmacol. 2013 May;65(5):665-72. Doi: 10.1111/Jphp.12033. Epub 2013 Feb 26. Alteration In P-Glycoprotein At The Blood-Brain Barrier In The Early Period Of Mcao In Rats.

Hu P, Wang J, Zhao Xq, Hu B, Lu L, Qin Yh. Mol Biol Rep. 2013 Feb;40(2):1429-41. Doi: 10.1007/S11033-012-2186-7. Epub 2012 Oct 17. Overexpressed C-Type Natriuretic Peptide Serves As An Early Compensatory Response To Counteract Extracellular Matrix...

Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...

Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.

Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.

Wu L, Zhang W, Tang Yh, Li H, Chen By, Zhang Gm, Deng Cq. Phytomedicine. 2010 Mar;17(3-4):233-40. Doi: 10.1016/J.Phymed.2009.07.021. Epub 2009 Sep 11. Effect Of Total Saponins Of "Panax Notoginseng Root" On Aortic Intimal Hyperplasia And The Expre...

Zhou Y, Tan X, Kuang W, Liu L, Wan L. Transl Res. 2012 Jun;159(6):464-72. Doi: 10.1016/J.Trsl.2011.09.007. Epub 2011 Oct 24. Erythromycin Ameliorates Cigarette-Smoke-Induced Emphysema And Inflammation In Rats.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.

Jiang Z, Han B, Li H, Li X, Yang Y, Liu W. Carbohydr Polym. 2015 Jul 10;125:53-60. Doi: 10.1016/J.Carbpol.2015.02.039. Epub 2015 Feb 26. Preparation And Anti-Tumor Metastasis Of Carboxymethyl Chitosan.

Chen Sz, Yao Hq, Zhu Sz, Li Qy, Guo Gh, Yu J. Oncol Lett. 2015 Feb;9(2):915-919. Epub 2014 Dec 4. Expression Levels Of Matrix Metalloproteinase-9 In Human Gastric Carcinoma.

Huang Z, Yang Y, Jiang Y, Shao J, Sun X, Chen J, Dong L, Zhang J. Biomaterials. 2013 Jan;34(3):746-55. Doi: 10.1016/J.Biomaterials.2012.09.062. Epub 2012 Oct 27. Anti-Tumor Immune Responses Of Tumor-Associated Macrophages Via Toll-Like Receptor 4 ...

Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Bear...

Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...

Ma X, Ma X, Ma Z, Sun Z, Yu W, Wang J, Li F, Ding J. Evid Based Complement Alternat Med. 2014;2014:710870. Doi: 10.1155/2014/710870. Epub 2014 Oct 15. The Effects Of Uygur Herb Hyssopus Officinalis L. On The Process Of Airway Remodeling In Asthmat...

Wang B, Liang S, Wang Y, Zhu Xq, Gong W, Zhang Hq, Li Y, Xia Cm. Plos Negl Trop Dis. 2015 Jan 15;9(1):E0003434. Doi: 10.1371/Journal.Pntd.0003434. Ecollection 2015. Th17 Down-Regulation Is Involved In Reduced Progression Of Schistosomiasis Fibrosi...

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16 Customer Q&As for Anti-Matrix metalloproteinase-9 MMP9 Antibody

Question

Would PA1357 anti-MMP9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

It shows on the product datasheet, PA1357 anti-MMP9 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-05-08

Question

Can you help my question with product PA1357, anti-MMP9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1357 anti-MMP9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-23

Question

We have been able to see staining in human neutrophil. Do you have any suggestions? Is anti-MMP9 antibody supposed to stain neutrophil positively?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

Based on literature neutrophil does express MMP9. Based on Uniprot.org, MMP9 is expressed in tibia, umbilical cord blood, b-cell, neutrophil, fibrosarcoma, blood, monocytic leukemia, among other tissues. Regarding which tissues have MMP9 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1480034, 1281792
Fibrosarcoma, Pubmed ID: 1400481, 7669817
Monocytic leukemia, Pubmed ID: 20800577
Neutrophil, Pubmed ID: 1645657, 1464361, 1653055
Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-04-02

Question

Is this PA1357 anti-MMP9 antibody reactive to the isotypes of MMP9?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

The immunogen of PA1357 anti-MMP9 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9(689-705aa NQVDQVGYVTYDILQCP), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-23

Question

My team were happy with the WB result of your anti-MMP9 antibody. However we have observed positive staining in neutrophil extracellular space using this antibody. Is that expected? Could you tell me where is MMP9 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

Based on literature, neutrophil does express MMP9. Generally MMP9 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP9 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1480034, 1281792
Fibrosarcoma, Pubmed ID: 1400481, 7669817
Monocytic leukemia, Pubmed ID: 20800577
Neutrophil, Pubmed ID: 1645657, 1464361, 1653055
Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-03-18

Question

Is a blocking peptide available for product anti-MMP9 antibody (PA1357)?

R. Yang

Verified customer

Asked: 2020-03-16

Answer

We do provide the blocking peptide for product anti-MMP9 antibody (PA1357). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-16

Question

Will anti-MMP9 antibody PA1357 work for WB with neutrophil?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

According to the expression profile of neutrophil, MMP9 is highly expressed in neutrophil. So, it is likely that anti-MMP9 antibody PA1357 will work for WB with neutrophil.

Boster Scientific Support

Answered: 2020-01-31

Question

Is there a BSA free version of anti-MMP9 antibody PA1357 available?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP9 antibody PA1357 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-08

Question

I see that the anti-MMP9 antibody PA1357 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-03

Question

Does anti-MMP9 antibody PA1357 work on canine WB with blood?

Verified Customer

Verified customer

Asked: 2019-05-06

Answer

Our lab technicians have not tested anti-MMP9 antibody PA1357 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MMP9 antibody PA1357 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-06

Question

I was wanting to use to test anti-MMP9 antibody PA1357 on human neutrophil for research purposes, then I may be interested in using anti-MMP9 antibody PA1357 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-03-26

Answer

The products we sell, including anti-MMP9 antibody PA1357, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-26

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neutrophil using anti-MMP9 antibody PA1357. Let me know if you need anything else.

K. Johnson

Verified customer

Asked: 2017-12-21

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-21

Question

I have attached the WB image, lot number and protocol we used for neutrophil using anti-MMP9 antibody PA1357. Please let me know if you require anything else.

M. Carter

Verified customer

Asked: 2017-12-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-11

Question

We are currently using anti-MMP9 antibody PA1357 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-26

Answer

The anti-MMP9 antibody (PA1357) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-26

Question

I would like using your anti-MMP9 antibody for interleukin-4 and interleukin-13 signaling studies. Has this antibody been tested with western blotting on mm453 cell lysate? We would like to see some validation images before ordering.

D. Anderson

Verified customer

Asked: 2014-10-23

Answer

We appreciate your inquiry. This PA1357 anti-MMP9 antibody is validated on rat embryo tissue lysate, mm453 cell lysate, hela cell lysate, smmc cell lysate, jurkat cell lysate, ht1080 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-10-23

Question

I was wanting to use your anti-MMP9 antibody for WB for human neutrophil on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human neutrophil identification?

S. Dhar

Verified customer

Asked: 2014-07-24

Answer

You can see on the product datasheet, PA1357 anti-MMP9 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human neutrophil in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-07-24

Size

Total: $315

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1357
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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