Mouse RANK / CD265 PicoKine™ Quick ELISA Kit

Mouse RANK / CD265 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 2pg/ml.

Product Info Summary

SKU: FEK0830
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse RANK / CD265 PicoKine™ Quick ELISA Kit

See all RANK/TNFRSF11A products

SKU/Catalog Number

FEK0830

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse RANK / CD265 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse RANK / CD265 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0830)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-P213

Sensitivity

<2 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0830 is reactive to Tnfrsf11a in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

FEK0830 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity
FEK0830-CAP 96-well plate precoated with anti-Mouse RANK/TNFRSF11A antibody 1
FEK0830-ST lyophilized recombinant Mouse RANK/TNFRSF11A standard 10 ng/tube
FEK0830-DA Biotinylated anti-Mouse RANK/TNFRSF11A detection antibody (50x) 130 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04000200040002000400020004000
O.D.0.0060.1090.2050.4320.7191.1841.6592.038

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2107191032218725978
Standard deviation11.9730.1946.4415.8%542.0553.79
CV (%)5.7%4.2%4.5%7.5%5.8%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of RANK/TNFRSF11A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121021619818620211.525.7%
Sample 271968570766669420.362.9%
Sample 31032104610901088106425.492.3%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf11a (Source: Uniprot.Org, NCBI)

Uniprot ID

O35305

Gene ID

21934

Gene Name

Tnfrsf11a

Full Name

Tumor necrosis factor receptor superfamily member 11A

Weight

66.621kDa

Alternative Names

CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator Tnfrsf11a|Ly109, ODFR, OFE, R, Rank, TRAN, TRANCE-R, mRANK|tumor necrosis factor receptor superfamily, member 11a, NFKB activator|tumor necrosis factor receptor superfamily member 11A|osteoclast differentiation factor receptor|receptor activator of NF-KB|receptor activator of NF-kappaB

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf11a, check out the Tnfrsf11a Infographic

Tnfrsf11a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf11a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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