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- Table of Contents
Tnfrsf11a antibody (A01064-3) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of HEPA1-6 cells using anti-Tnfrsf11a antibody (A01064-3).
Overlay histogram showing HEPA1-6 cells stained with A01064-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf11a Antibody (A01064-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing HEPA1-6 cells stained with A01064-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf11a Antibody (A01064-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-RANK/Tnfrsf11a Picoband® Antibody
Tnfrsf11a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Tnfrsf11a antibody was positively detected by WB in:
rat thymus tissue, mouse thymus tissue, mouse RAW2647 whole cell, mouse SP20 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Tnfrsf11a antibody was positively detected by FC in:
HEPA1-6 cell, RH-35 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-RANK/Tnfrsf11a Picoband® Antibody
View all TNFRSF11A Antibodies
View all TNFRSF11A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse RANK/Tnfrsf11a recombinant protein (Position: Q30-R203).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated Molecular Weight
66.6 kDa
Background of Tnfrsf11a
Receptor activator of nuclear factor κ B (RANK), also known as TRANCE receptor or TNFRSF11A, is a member of the tumor. It is mapped to 1; 1 E2.1. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.
Cite This Product
Anti-RANK/Tnfrsf11a Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01064-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
95%
High Homology
Human
80%
Moderate Homology
Sheep
73%
Moderate Homology
Cow
71%
Moderate Homology
Monkey
71%
Moderate Homology
Dog
68%
Low homology
Zebrafish
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Tnfrsf11a antibody
Flow Cytometry (FC) protocol for Tnfrsf11a antibody
Publications
Application:
Species:
Effect of intervention initiation timing of pulsed electromagnetic field on ovariectomy-induced osteoporosis in rats
Published: 2017-05-16
Journal: BIOELECTROMAGNETICS
Application: WB
Impact Factor: 1.93
Species: Rat
PMID: 28510268
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Product Promise
We promise Anti-RANK/Tnfrsf11a Picoband® Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01064-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026