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Product Info Summary
| SKU: | A01064-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RANK/Tnfrsf11a Picoband® Antibody
SKU/Catalog Number
A01064-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RANK/Tnfrsf11a Picoband® Antibody catalog # A01064-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RANK/Tnfrsf11a Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01064-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse RANK/Tnfrsf11a recombinant protein (Position: Q30-R203).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01064-3 is reactive to Tnfrsf11a in Mouse, Rat
Observed Molecular Weight
80 kDa
Calculated molecular weight
66.6 kDa
Background of Tnfrsf11a
Receptor activator of nuclear factor κ B (RANK), also known as TRANCE receptor or TNFRSF11A, is a member of the tumor. It is mapped to 1; 1 E2.1. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01064-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat thymus tissue, mouse thymus tissue, mouse RAW2647 whole cell, mouse SP20 whole cell
FCM: HEPA1-6 cell, RH-35 cell
Validation Images & Assay Conditions
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Western blot analysis of Tnfrsf11a using anti-Tnfrsf11a antibody (A01064-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates,
Lane 4: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf11a antigen affinity purified polyclonal antibody (Catalog # A01064-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf11a at approximately 80KD. The expected band size for Tnfrsf11a is at 66KD.
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Flow Cytometry analysis of HEPA1-6 cells using anti-Tnfrsf11a antibody (A01064-3).
Overlay histogram showing HEPA1-6 cells stained with A01064-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf11a Antibody (A01064-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Flow Cytometry analysis of RH-35 cells using anti-Tnfrsf11a antibody (A01064-3).
Overlay histogram showing RH-35 cells stained with A01064-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf11a Antibody (A01064-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-RANK/Tnfrsf11a Picoband® Antibody (A01064-3)
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