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Product Info Summary
| SKU: | A06059 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-POLR3A Antibody
SKU/Catalog Number
A06059
Size
100μl
Form
Liquid
Description
Boster Bio Anti-POLR3A Antibody catalog # A06059. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLR3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06059)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RPC1. AA range:31-80
Reactive Species
A06059 is reactive to POLR3A in Human, Monkey, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
155.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06059 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using POLR3A Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from COS and HUVEC cells, using RPC1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using RPC1 antibody.
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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-POLR3A Antibody (A06059)
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Customer Q&As
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7 Customer Q&As for Anti-POLR3A Antibody
Question
I see that the anti-POLR3A antibody A06059 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-09
Question
Our lab want to know about to test anti-POLR3A antibody A06059 on human buccal mucosa cell for research purposes, then I may be interested in using anti-POLR3A antibody A06059 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The products we sell, including anti-POLR3A antibody A06059, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-30
Question
Would A06059 anti-POLR3A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
As indicated on the product datasheet, A06059 anti-POLR3A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-02
Question
Here is the WB image, lot number and protocol we used for buccal mucosa cell using anti-POLR3A antibody A06059. Please let me know if you require anything else.
K. Lewis
Verified customer
Asked: 2018-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-POLR3A antibody A06059. Let me know if you need anything else.
S. Banerjee
Verified customer
Asked: 2017-07-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-13
Question
I was wanting to use your anti-POLR3A antibody for WB for human buccal mucosa cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human buccal mucosa cell identification?
D. Baker
Verified customer
Asked: 2015-06-30
Answer
You can see on the product datasheet, A06059 anti-POLR3A antibody has been validated for WB on human, monkey, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human buccal mucosa cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-30
Question
Is this A06059 anti-POLR3A antibody reactive to the isotypes of POLR3A?
R. Carter
Verified customer
Asked: 2014-02-28
Answer
The immunogen of A06059 anti-POLR3A antibody is Synthesized peptide derived from the N-terminal region of human POLR3A. at AA rangle: 1-80. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-28
